SAFETY INFORMATION
Lattice Lightsheet 7
User Interface - Mounting and Dismounting Components
ZEISS
04/2021 V_01
000000-2324-960
37
The heated plate (Fig. 21/9) and tray (Fig. 21/7) of the heating chamber are integral part of the stage. The
tray contains the docking block (Fig. 21/
2
) to connect the gas from the Gas mixer and Humidifying column
(Fig. 21/
6
) as well as the electric supply from the temperature controller and the humidity sensor (Fig. 21/
3
).
The electric cable (Fig. 21/
4
) connects to the supply plug (Fig. 21/
10
) at the left front of the Lattice
Lightsheet 7, whereas the hydraulic pipe (Fig. 21/
5
) is feeded through the right slit recess (Fig. 21/
12
).
1.13.6.2
Gas Incubation System
The gas incubation system consists of the following components:
•
Gas mixer (Fig. 22)
•
Humidifying column (Fig. 23)
•
Heated tubing package (Fig. 24)
The Gas Incubation System (gas mixer, humidifying column and heated tubing package) provides defined gas
control for live cell imaging experiments. It is intended to be used with stage top incubator of the
Lattice Lightsheet 7. The gas incubation system mixes the required gas ratios in the output and actively
humidifies this mixed gas output before the gas enters the incubation chamber. The system can control the
CO
2
concentration and humidity. It can also downregulate the amount of O
2
in the gas mixture to create
hypoxia via the N
2
supply.
Gas Mixer
The Gas Mixer (Fig. 22) is the gas mixing and control unit of the ibidi Gas Incubation System. In addition to
the gas mixture, the humidity within the incubation chamber is controlled by a feedback loop using a humidity
sensor located in the incubation chamber. The LEDs on the front of the gas mixer (Fig. Fig. 22) indicate the
status of the channels: connection to the power supply, gas control (N
2
, CO
2
and air), and humidity control.
LED off
channel inactive
LED on
channel active
LED blinking
channel error
On the back of the Gas Mixer, all connections for cables and tubing are provided.
The Gas Incubation System takes in pure CO
2
gas and pressurized air, as well as pure N
2
gas. Pressurized air
can be supplied by the lab gas line or a small air pump or compressor. CO
2
and N
2
can be supplied by
corresponding lab gas lines or appropriate gas supply bottles. The pressure applied should be around 1 bar.
Electrical Connections
1.
Connect the power plug (Fig. 22/
15
) via the power cable to the mains power 3-point connector.
2.
Connect the heating plug (Fig. 22/
13
) to the electric plug (Fig. 24/
3
) of the humidifying column.
3.
Connect the Sensor Plug (
Fig. 22
/14) to the Gas supply line plug (
Fig. 2
/12) of the Lattic Lightsheet 7
4.
Connect the CAN bus 1 plug (
Fig. 22
/6) to the CAN bus 2 plug (
Fig. 19
/6) of the Temperature controller.
5.
Connect the CAN bus 2 plug (
Fig. 22
/16) to the Gas CAN bus plug (
Fig. 2
/11) of the Lattice Lightsheet 7.
