SAFETY INFORMATION
ZEISS
User Interface - Mounting and Dismounting Components
Lattice Lightsheet 7
30
000000-2324-960
04/2021 V_01
The first immersion before mounting the sample must be supplied manually. During this priming process air-
bubbles will be removed from the immersion system. Proceed as follows (Fig. 14):
1.
Turn the stopcock (Fig. 14/
9
) connected to the syringe (Fig. 14/
10
) to the open position (cock aligns to
the flow direction).
2.
Fill the syringe (Fig. 14/
10
) with water by pulling steadily on the syringe piston. The valve (Fig. 14/
6
)
ensures that water can only flow from the immersion container (Fig. 14/
8
) to the syringe. Make sure the
immersion container is sufficiently filled and the tubing dips into the water. If accidentally too much air
was sucked in, disconnect the syringe, empty it, reconnect it and refill it.
3.
To remove any air bubbles from the tubing (Fig. 14/
4
), press water out by slowly pushing the syringe
piston. Note that due to the valve (Fig. 14/
6
) and pump (Fig. 14/
7
) water can only flow to the relay optics
(Fig. 14/
2
) via the Luer-Lock coupling (Fig. 14/
5
). Observe water level rising at the meniscus lens and stop
pressing the piston before water spills from the lens to the relay optics holder (Fig. 14/
3
).
4.
Check for air-bubbles within the water immersion layer on top of the meniscus lens. If air bubbles are
noticeable suck off the immersion by using a suitable filter paper and repeat step 3 and 4 until no air
bubbles are visible.
5.
Turn the stopcock (Fig. 14/
9
) to the closed position (cock aligns vertically to the flow direction).
6.
System can now operate via the pump and the ZEN software (Fig. 14/
5
).
It is advised to fix the syringe with the hydraulic tube on the inner side of the front door with the
appropriate clamps (Fig. 13).
