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6
•
Operation
Page 96
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
6.5
Preparing the Device for Operation
This section gives information on any additional steps that are required
to prepare the device for operation and sample analysis.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE
Flush the system flow path thoroughly before operating the device for
the first time:
•
When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the
Vanquish
System Operating Manual
.
•
When operating the device with a flow cell that was stored, the flow
cell may be filled with solvent. Use solvents that are miscible with
this solvent, or use an appropriate intermediate solvent.
Before Starting Sample Analysis
Before starting an analysis:
•
Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
•
Verify that the doors of all modules in the Vanquish system are
closed.
•
Observe the guidelines for use of flow cells in
.
•
Make sure that the chromatography system is properly equilibrated
(see further down).
System Equilibration
System equilibration should include the following operations:
•
Purging the pump (
all
channels, including those not used for the
application)
•
Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...