8
•
Troubleshooting
Page 160
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Message and Code
Description and Remedial Action
Code 8049 Command rejected - close
front door first
It is not possible to start data acquisition with the front doors open.
Close the doors and try again.
Code 8051 Flow cell detected. It is
recommended executing the
command CalibrateRaman
A new flow cell was found. It is recommended to perform a Raman
wavelength calibration (see
Performing a Wavelength Calibration
); without calibration, wavelengths may deviate from the
actual wavelength by up to a few nanometers.
Code 8064 PMT x signal overflow in
<channel name>
Code 8065 PMT x signal overflow
during scan
With x = PMT 1 or 2
Signal overflow in PMT 1 or 2 occurred. Repeat the run or scan with a
lower sensitivity or lower concentration of the sample.
Code 8071 PMT Sensitivity autorange
for <channel name> -new Sensitivity
is x
With x = The new sensitivity value after the adjustment.
The detector had to automatically reduce the Sensitivity. Select a
suitable Sensitivity (see
Sensitivity (Detector Sensitivity) (
and repeat. x indicates the new sensitivity value after the adjustment.
Use this value, or a smaller value, in your method when you expect
similar concentrations.
Code 8078 PMT Autorange for
<channel name> not possible -
already minimal Sensitivity
The detector could not automatically reduce the Sensitivity, as the
Sensitivity setting is already set to a minimum. Repeat with a lower
concentration of the sample, or smaller injection volume.
Code 8087 System Interlink error
The System Interlink connection got lost. Check that all System
Interlink cables on the Vanquish system are properly connected.
Code 8093 Not initialized
The detector is not initialized. Verify that a flow cell is installed
correctly and that the eluent composition in the flow cell does not
change and no air bubbles are present. Then open and close the
doors, or press
INIT
on the keypad.
Code 8100 - Code 8109 Calibration of
xx failed.
With x = Name of the component for which calibration failed.
Cause details
•
Raman calibration failed.
•
The flow cell may be installed incorrectly.
•
The flow cell may be contaminated.
Remedial actions
•
Observe the conditions for wavelength calibration (see
(see
Performing a Wavelength Validation (
•
Verify that the flow cell is installed correctly and that the screws are
tightened finger-tight.
•
Clean the flow cell (see
) if
necessary.
•
Make sure that the flow cell is properly equilibrated. Make sure that
you use degassed solvents.
If the procedure is repeatedly interrupted, contact Technical Support.
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...