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Troubleshooting
Page 164
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Symptom
Possible Cause
Remedial Action
Spikes
Xenon flash lamp old,
defective, or not
properly installed
Contact Technical Support.
Fluorescence detector
flow cell temperature
near boiling point of
mobile phase
Turn off flow cell temperature control or reduce the
temperature setting.
Inappropriate device
settings
Improper wavelength,
e.g., in a UV spectrum
flank
Choose a detection wavelength or an excitation/
emission wavelength pair that is located near the
apex of the spectrum.
The Sensitivity of the
fluorescence detector
was automatically
reduced
Select a lower Sensitivity for your application.
High baseline drift
Absorption of eluent
changes when gradient is
run
Absorbing additives may change the absorption
spectrum, depending on the solvent. Consider
varying additive concentrations to level the drift.
Non-periodic baseline
fluctuation, high noise
Xenon flash lamp in
fluorescence detector
too old
Contact Technical Support.
Flow cell in fluorescence
detector does not reach
set temperature
Set flow cell temperature
too high or too low
At high flow rates, the flow cell may not reach the
desired temperature. Select a lower flow cell
temperature.
The temperature might also be too low, e.g. below
ambient temperature. Correct the temperature
setting or switch off temperature control.
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...