ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 164 / 188

8 • Troubleshooting
Page 164 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Symptom Possible Cause Remedial Action
Spikes Xenon flash lamp old,
defective, or not
properly installed Contact Technical Support.
Fluorescence detector
flow cell temperature
near boiling point of
mobile phase Turn off flow cell temperature control or reduce the
temperature setting.
Inappropriate device
settings Improper wavelength,
e.g., in a UV spectrum
flank Choose a detection wavelength or an excitation/
emission wavelength pair that is located near the
apex of the spectrum.
The Sensitivity of the
fluorescence detector
was automatically
reduced Select a lower Sensitivity for your application.
High baseline drift Absorption of eluent
changes when gradient is
run Absorbing additives may change the absorption
spectrum, depending on the solvent. Consider
varying additive concentrations to level the drift.
Non-periodic baseline
fluctuation, high noise Xenon flash lamp in
fluorescence detector
too old Contact Technical Support.
Flow cell in fluorescence
detector does not reach
set temperature Set flow cell temperature
too high or too low At high flow rates, the flow cell may not reach the
desired temperature. Select a lower flow cell
temperature.
The temperature might also be too low, e.g. below
ambient temperature. Correct the temperature
setting or switch off temperature control.