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6
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Operation
Page 116
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Extending the lamp lifetime
To extend the lamp lifetime, you can do the following:
•
Turn off the lamp by stopping data acquisition if you are not
interested in the baseline. It is not generally required that the lamp
remains turned on during the entire chromatographic separation.
•
Change the lamp mode during a chromatographic run, for example,
from
LongLife
mode (between the peaks) to
Standard
or
HighPower
mode (for the interesting peaks). This extends the lamp lifetime,
without any loss in sensitivity in the important areas.
Use the Instrument Method Wizard to set the basic lamp mode.
After completion of the wizard, open the method in the Instrument
Method Editor. In the Module View for the detector, on the
Timetable
tab page, you can define the times for switching the lamp
mode and other parameters.
For an example of smart use of the xenon flash lamp modes, refer to the
Fluorescence Method Development Handbook .
HighPower Mode
The lamp flashes at the highest frequency of 300 Hz. The sensitivity is
roughly twice the sensitivity in
Standard
mode. The expected lifetime of
the lamp is approximately 1300 hours.
This mode is recommended for applications that require highest
sensitivity.
Standard Mode
The lamp flashes at a medium frequency of 100 Hz. The expected
lifetime of the lamp is approximately 4000 hours. This mode supports
data collection rates up to 100 Hz.
This mode is recommended for applications that require a high
sensitivity.
LongLife Mode
The lamp flashes at a lower frequency of 20 Hz. When the lamp is
operated in this mode, the lamp lifetime is approximately four times the
lifetime in
Standard
mode. The expected lifetime of the lamp is
approximately 16000 hours. However, note that signal-to-noise ratio is
reduced to roughly the half compared to
Standard
mode. This mode
only supports data collection rates up to 20 Hz.
This mode is recommended for regions of the chromatogram when
signal-to-noise is less important, for example:
•
when no peaks of interest elute
•
during the wash and re-equilibration phase
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...