ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 117 / 188

6 • Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 117
6.9.10 Baseline Behavior
If wavelengths are switched or the sensitivity, filter wheel, or PMT
setting are changed during the method, the chromatogram may show
baseline jumps because of the background fluorescence at the new
wavelength setting. Three baseline behavior mode settings define how
the baseline behaves in these cases:
• Zero : sets the baseline to zero
• Append : appends the baseline to the previous signal
• Free : lets the baseline "jump" to the current absolute value
TIP You can perform Autozero command (automatic null balancing) any
time, that is, the intensity measured at the time of the autozero is
subtracted from any intensities measured thereafter. Always select a
time for null balancing at which no sample flows through the flow cell.
The values are stored and can be reset using the
ClearAutozero
command.