![background image](http://html1.mh-extra.com/html/thermofisher-scientific/vanquish-vc-d50/vanquish-vc-d50_operating-manual_1105630110.webp)
6
•
Operation
Page 110
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Determining the optimum sensitivity
Determine the optimum sensitivity in a separate sample run after you
have determined the optimum wavelengths as described below.
•
Use a sample (standard) with the maximum expected concentration
of the analytes.
•
Select a sensitivity at which a saturation is not expected (for
example, 1 or 2).
•
If the peak heights of the individual peaks in a chromatogram differ,
you can improve the signal-to-noise ratio of the smaller peaks by
switching the sensitivity during the run and between the peaks with
the help of a timetable.
•
Always use the same PMT setting for determining the sensitivity that
is used for the analysis.
An instrument method example is available in the
Chromeleon Help
.
Follow these steps
1. Open the method you want to optimize in the
Script Editor
. The
required commands must be added to the method manually.
2. Add a
ClearMaxPMTSaturation
command at the beginning of the
run.
3. Add a
Log
command for the
MaxPMTSaturation
property after the
peak maximum of an expected peak (or group of peaks) to log the
maximum saturation value at this time in the Audit Trail (see figure
below).
4. Add a
ClearMaxPMTSaturation
command shortly after, before the
next peak elutes, to reset the
MaxPMTSaturation
value to zero (see
figure below).
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...