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7
•
Maintenance and Service
Page 134
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
7.6
Performing a Wavelength Validation
You can validate the wavelength calibration using water (Raman
measurement). If validation fails, that is, if the measured values
significantly deviate from the calibration values, you can perform a
wavelength calibration using water (Raman measurement). The light
spectrum of the xenon flash lamp is used to validate the excitation
wavelength.
When
•
After (re)installation of the detector
•
After wavelength calibration
Preparations
The following conditions
must
be met for both wavelength validation
and Raman wavelength calibration:
•
The system is equilibrated and environmental conditions are stable.
•
There are no air bubbles in the flow cell. We recommend degassing
the water. Set a flow rate of 0.5 mL/min and wait about 15 minutes
before you begin, until the baseline is stable.
•
The water has no impurities. Always use fluorescence-grade water
and consider using a filter or a suitable column before the detector.
To perform a wavelength validation
You can perform wavelength validation via Chromeleon. Wavelength
validation can take a few minutes. During this time, data acquisition is
not possible.
1. Execute the
ValidateRaman
command to start the validation.
The following message appears in Chromeleon:
Make sure that
¨
the system is equilibrated and conditions are stable
¨
fluorescence-grade water is flowing through the cell
¨
the water is degassed (no air bubbles) and free of particles
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...