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6
•
Operation
Page 120
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Situation after Shutdown
If no additive is used
If an additive is used
Device and flow cell shall
be transported or shipped
after shutdown
Flush the system with
isopropanol.
Flush the system first with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the flow cell are not
miscible with water, use
an appropriate
intermediate solvent.
Afterward, flush the
system with isopropanol.
NOTICE
Residual samples, impurities from the column or buffers with high salt
concentrations can deposit in the flow cell. This can lead to damage of
the flow cell. In addition, solvents containing acid can damage the flow
cell.
•
Always flush the flow cell with an appropriate solvent before
interrupting operation.
•
Fill the flow cell with pure isopropanol using the flushing and
injection kit.
•
The flow cell should
not
be filled with pure water to avoid the
growth of algae. If you want to fill the flow cell with water, you need
to add 10% HPLC-grade isopropanol.
5. Turn off the pump flow to the device. Wait until the system pressure
is down to zero before you continue the shutdown of the device.
6. Disconnect the capillaries from the flow cell inlet and outlet.
7. After removing the flow connections, protect the flow cell inlet and
outlet ports with the plugs supplied with the flow cell. Protect the
inlet capillaries with caps.
8. The step depends as follows:
Situation
Steps
Device and all other system modules
remain in the system stack and are to be
turned off
Turn off the system with the system
power button on the system base.
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...