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6
•
Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Page 111
Figure 34: MaxPMTSaturation parameter monitoring
5. Repeat steps 3 through 4 for all peaks.
6. Run a sample and read out the
MaxPMTSaturation
values in the
Chromeleon software and evaluate the results:
Value
What you should do…
< 30%
Increase the Sensitivity. The following is a rough guideline:
< 30 %: by one stage
< 15 %: by two stages
Then repeat the optimization, starting with step 1.
30% - 80%
The Sensitivity value is optimal.
80% - 99%
The Sensitivity should be reduced by one stage to prevent unexpected
saturation when concentration varies.
≥ 100%
Reduce the Sensitivity by at least one stage. In most cases, the detector
has reduced Sensitivity automatically ("autoranging"). Check the Audit
Trail for the smallest Sensitivity value after the autoranging and repeat
the optimization by using this value, starting with step 1.
If you do not know the expected concentrations of the analytes, reduce
the sensitivity even at smaller values of
MaxPMTSaturation
.
TIP
•
The maximum saturation indicated by
MaxPMTSaturation
always
refers to the signals of both PMTs at the parameter settings for all
channels.
•
You can completely turn off fluorescence detection by setting the
sensitivity to
Off
. This may be required, for example, if you want to
record the incoming signal from a UV detector for a sample of high
concentration, or to suppress an extremely high peak that is not of
interest for you but triggers an automatic adjustment of the
sensitivity.
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...