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6
•
Operation
Page 104
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
6.8
Important Operating Parameters
The commands and parameters described in the table should be
considered for simple routine operation of the device. You can usually
access these parameters from the Chromeleon user interface.
If a parameter listed below is not available in the Chromeleon software,
consider updating the firmware and Chromeleon version.
TIP
The Instrument Method Wizard provides different parameter view
modes. Depending on the parameters to be set, you can select the
desired view mode (for example,
Easy
or
Advanced
).
For more information, refer to
Chromeleon Help and documents
.
Parameter
Description
Emission signal
parameters
The Emission signal channels (
Emission_x
) are the signal
channels for recording an excitation/emission wavelength
pair. Signal parameters are settable for each signal channel
separately. For guidelines on selecting the signal
parameters, see
Optimizing the Performance of the Device
Excitation wavelength
Sets the excitation wavelength in nm. Select the excitation
wavelength at the absorption maximum of the sample
component to be analyzed.
Emission wavelength
Sets the emission wavelength in nm. The emission
wavelength must be at least 20 nm above the excitation
wavelength.
ZeroOrder
: The grating of the emission monochromator is
set to the position of zero order. For details, see
.
Sensitivity
Sets the detector sensitivity, see
Data collection rate
Sets the number of data points per second (Hz) that the
Chromeleon software collects from the detector and stores
as raw data.
For further information, see
Response Time (Single-Channel Mode) (
.
Lamp mode
Sets the mode of operation for the xenon flash lamp. A
higher frequency of flashes improves the baseline noise and
thus improves sensitivity. A lower flash frequency extends
the lifetime of the lamp.
For details, see
.
Effective lamp age
Provides information about the lamp operating hours to
help you in estimating the remaining lamp lifetime. For
further information, see
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...