ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 27 / 188

2 • Safety
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 27
2.4 Solvent and Additive Information
2.4.1 General Compatibility
To protect optimal functionality of the Vanquish system, observe these
recommendations on the use of solvents and additives:
• The system must be used with reversed-phase (RP) compatible
solvents and additives only.
• Use only solvents and additives that are compatible with all parts in
the flow path.
TIP In a Vanquish Core system, normal-phase (NP) compatible solvents
and additives may be used if the VC-pumps and the VC-autosamplers are
modified with the components from the Normal-Phase (NP) kit. Refer to
the
Operating Manuals for the pumps and autosamplers.
2.4.2 Allowed pH Ranges
Allowed pH ranges (standard system configuration):
System
(Standard
Configuration) Allowed
pH ranges Remarks
Vanquish Core 1-13 • pH values of 2 or less : The application time should
be as short as possible. Flush the system
thoroughly after these applications.
• pH values higher than 9.5 with optical detectors :
Avoid using mobile phases with a pH value higher
than 9.5 together with optical detectors. This can
impair the functionality and optical performance
of the detector flow cell.
• pH values higher than 12 : May affect
electrochemical detection. Before using highly
alkaline solvents for flushing the system,
disconnect the detector from the system.
• Mobile phases containing ammonium hydroxide : In
rare cases, a shortened lifetime of reversed-phase
(UHMW-PE) piston seals has been observed with
high pH, ammonium hydroxide containing mobile
phases and prolonged exposure.
Vanquish
Horizon
Vanquish Flex
2-12