ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 73 / 188

5 • Installation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 73
Figure 23: Connecting the inlet capillary from the column compartment
(example)
1. Route the inlet capillary from the column compartment through the
guide hole in the device enclosure. Use the guide hole that is next to
the column compartment.
TIP Always keep the capillary connection between the column
compartment and the flow cell as short as possible to minimize peak
dispersion (i.e. peak broadening effects due to extra dispersion volume).
2. If the column compartment is located to the right of the detector
Route the capillary through the bottom recess in the partition panel.
Make sure that you use the suitable partition panel plug for the
capillary.
TIP For capillaries with small outer diameter, the plug with slit secures
the capillary properly. For capillaries that do not fit in the plug with slit,
such as an insulated inlet capillary, use the rotating plug.
3. Connect the capillary to the flow cell inlet. Make sure that you
secure the inlet capillary appropriately in the capillary clip on the
front panel.
Connecting the inlet capillary from the UV/VIS detector
Connect the capillary between UV/VIS detector flow cell outlet and
fluorescence detector flow cell inlet. The figure shows the steps to
establish the connection from a diode array detector as an example. The
fluorescence detector must be the last detector connected in the flow
path.