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6
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Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Page 101
For an example of method development using the Zero Order Mode,
refer to the Fluorescence Method Development Handbook.
TIP
To activate the Zero Order Mode in the Chromeleon software, set the
EmWavelength
property to
ZeroOrder
.
See also
2
Filter Wheel (VF detectors only) (
6.7.4
Single Spectrum Scan
During a Single Spectrum Scan, the excitation monochromator or
emission monochromator (or both simultaneously) moves over a
settable wavelength range, while the intensity of the fluorescence
signals is measured and recorded continuously for each wavelength. This
allows you, for example, to record spectra for determining the optimum
emission and excitation wavelengths. There are three different scan
modes: excitation scan, emission scan, and synchronous scan.
To achieve stable conditions in the flow cell, it is recommended stopping
the pump flow or have the pump deliver at a very low flow rate. If the
scan can be completed during the elution of a peak and the scan is
significantly faster than the full width at half maximum of the peak, the
pump flow can be kept constant. For further information, refer to the
Chromeleon Help
.
Recording a baseline spectrum
A baseline spectrum with the same parameters can be recorded for all
scan modes. The baseline spectrum is stored in the Chromeleon
software and automatically subtracted from the recorded spectra. The
result is a difference spectrum, that is, the autofluorescence of the used
solvent is eliminated. Note that Chromeleon issues a warning if the
baseline spectrum is missing.
Whenever the parameters for a scan change, the background spectrum
is automatically deleted and a new spectrum must be recorded. You can
delete a baseline spectrum any time by using the
ClearBaseLine
command.
Excitation Scan
The wavelength on the emission monochromator is kept constant, while
the excitation monochromator scans a wavelength range. The result is
an excitation spectrum of the sample.
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...