ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 115 / 188

6 • Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 115
TIP For best possible combinations of data collection rate, response time
and peak width, enable the Link data collection parameters check box in
the detector settings of the Chromeleon Instrument Method Wizard or
Instrument Method Editor.
See also
2
Lamp Mode (
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page 115)
6.9.8 Flow Cell Temperature
The temperature of a sample influences its fluorescence. Thus, a
problem with fluorescence detection is that the results can strongly be
influenced by fluctuations in ambient temperature. Vanquish
fluorescence detectors allow you to heat the flow cell and thus keep
temperatures inside the flow cell at a constant level, even if ambient
temperatures change.
In most analytes, the fluorescence of a sample decreases as temperature
increases. Therefore, the temperature must not be selected too high.
However, it must be above the temperature inside the detector optics,
which is influenced by the ambient temperature. Therefore, select a
temperature that is roughly 15°C above the expected ambient
temperature.
Example : The expected maximum temperature in the laboratory is 27°C
(80.6 F). Set the flow cell temperature to 42°C (107.6 F).
TIP Thermo Fisher Scientific recommends always recording the
FLD_FlowCell signal channel. If a problem occurs, the temperature
channel can provide helpful information to identify and eliminate the
source for the problem.
6.9.9 Lamp Mode
The detector offers three different flash frequencies for the xenon flash
lamp. Selecting a different lamp mode during phases when no peaks of
interest elute can extend the lamp lifetime.
When calculating the lamp lifetime, only the time when the lamp flashes
is taken into account. This roughly corresponds to the acquisition time.