3
Data System Instrument Configuration
Configuring the Signal Polarity for the Accela AS in Xcalibur
28
Accela Pump Hardware Manual
Thermo Scientific
Figure 21.
Accela Pump dialog box (Xcalibur)
5. Click
OK
to close the Accela Pump dialog box.
6. Click
Done
to save the configuration and close the Instrument Configuration window.
Configuring the Signal Polarity for the Accela AS in Xcalibur
During an injection sequence, the pump issues a Pump Ready signal and an Injection Hold
Release signal to the autosampler.
When you configure the input signal polarities for the Accela Autosampler, select the Pump
ready active high check box and the Injection hold release active high check box (see
Figure 22
). If you do not select these two check boxes, the autosampler does not recognize the
input signals from the Accela Pump, and it does not begin an injection sequence when you
start a sequence.
After you submit a run, the Accela Autosampler waits for a Pump Ready signal from the
pump. The pump issues this signal after its pressure transducer monitors a stable
backpressure. When the autosampler receives this signal, it begins an injection sequence.
After the autosampler begins the injection sequence, it waits for an Injection Hold Release
signal from the pump. The pump issues this release signal when its piston cam returns to the
start position. When the autosampler receives the Injection Hold Release signal, the injection
valve switches to the inject position, allowing the contents of the sample loop to be swept onto
the LC column.
