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Contents

Thermo Scientific

Accela Pump Hardware Manual

xi

Baseline Noise at Low Pressures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Insufficient Solvent Flow from the Pump Outlet. . . . . . . . . . . . . . . . . . . . . . . . 89

Pump Cavitation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Blocked Proportioning Valve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Blocked Check Valve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Irreproducible Retention Times . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Chapter 8

Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91

Liquid Displacement Assembly (LDA) Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Accela Pump Accessory Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Accela System Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Solvent Interconnect Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Other Parts and Assemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Appendix A Discontinued Versions of the Accela Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101

Installing the Check Valves in the Discontinued LDA. . . . . . . . . . . . . . . . . . . 101

Installing the Check Valves and Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Installing the Screw-In Check Valves (discontinued version) . . . . . . . . . . . . 102

Installing the Components of the Discontinued LDA Stack . . . . . . . . . . . . . . 104

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107

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Summary of Contents for TM-740

Page 1: ...Accela Pump Hardware Manual 60157 97000 Revision D February 2009 ...

Page 2: ...l information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result ...

Page 3: ...e ordered from Thermo Fisher Scientific or one of its authorized representatives Accela Pump EMC Directive 89 336 EEC 92 31 EEC 93 68 EEC EMC compliance has been evaluated by TUV Rheinland of North America Inc Low Voltage Safety Compliance This device complies with Low Voltage Directive 73 23 EEC and harmonized standard EN 61010 1 2001 IEC 61010 1 2002 UL 61010 A 1 2004 CAN CSA 22 2 61010 1 2004 F...

Page 4: ...ulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturing ...

Page 5: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Page 6: ...ieurs compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont di...

Page 7: ...dati si raffreddino prima di effetturare l intervento di manutenzione Fire Use care when operating the system in the presence of flammable gases Feuer Beachten Sie die einschlägigen VorsichtsmaBnahmen wenn Sie das System in Gegenwart von entzündbaren Gasen betreiben Incendie Agir avec précaution lors de l utilisation du système en présence de gaz inflammables Fuego Tenga cuidado al operar el siste...

Page 8: ...s and proper procedures to dispose waste oil Heat Before servicing the instrument allow any heated components to cool Fire Use care when operating the system in the presence of flammable gases Eye Hazard Eye damage could occur from splattered chemicals or flying particles Wear safety glasses when handling chemicals or servicing the instrument General Hazard A hazard is present that is not included...

Page 9: ...king and Inspecting the Instrument 11 Making the Initial Instrument Preparations 12 Making the Initial Back Panel Connections 13 Connecting the Power Cable 14 Connecting the USB Cable 14 Connecting the System Interconnect Cable 15 Connecting the Low Pressure Solvent Lines 17 Assembling the Solvent Reservoir Bottles 17 Installing the Super Flangeless Fittings 19 Connecting the Pump to the Autosampl...

Page 10: ...nts after Disassembling the LDA 58 Cleaning the Check Valves 58 Cleaning the Pistons 58 Cleaning the Displacement Chambers and the Inlet Module 59 Removing the LDA from the Pump 60 Disassembling the LDA 64 Removing the Holding Hooks Bushings and Pistons from the LDA Body 64 Removing the Stacked Components from the LDA Body 66 Replacing the Primary Pistons Seals 67 Assembling the Liquid Displacemen...

Page 11: ...ories and Replacement Parts 91 Liquid Displacement Assembly LDA Parts 92 Accela Pump Accessory Kit 97 Accela System Kit 97 Solvent Interconnect Kit 98 Other Parts and Assemblies 98 Appendix A Discontinued Versions of the Accela Pump 101 Installing the Check Valves in the Discontinued LDA 101 Installing the Check Valves and Holders 101 Installing the Screw In Check Valves discontinued version 102 I...

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Page 13: ...ted Guide Accela Getting Started with Xcalibur Guide Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol IMPORTANT H...

Page 14: ...trols the movement of the pistons contains moving parts that can pinch your fingers White insulated electrical wires connect the actuating coils at the ends of the proportioning valves to the pump as shown below Take care not to pinch these wires when you remove the liquid displacement assembly LDA from the front panel of the pump to perform maintenance Insulated wires Actuating coil Drive arm mec...

Page 15: ...of a typical sample and standard mixture well documented with system conditions for future reference Careful comparison of retention times peak shapes peak sensitivity and baseline noise can provide valuable clues to identifying and solving future problems Chemical Toxicity Although the large volume of toxic and flammable solvents used and stored in laboratories can be quite dangerous do not ignor...

Page 16: ...emember that some solvents are corrosive to stainless steel Solvent Disposal Make sure you have a solvent waste container or other kind of drain system available at or below the benchtop level Most solvents have special disposal requirements and should not be disposed of directly down a drain Follow all governmental regulations when disposing of any chemical High pressure Systems and Leaks LC syst...

Page 17: ...o mssupport thermo com and click Customer Manuals in the left margin of the window Y To suggest changes to documentation or to Help Complete a brief survey about this document by clicking the link below Thank you in advance for your help Send an e mail message to the Technical Publications Editor at techpubs lcms thermofisher com Phone 800 532 4752 Fax 561 688 8736 E mail us techsupport analyze th...

Page 18: ......

Page 19: ...uction The Accela Pump is a member of the Accela family of ultra high performance liquid chromatography instruments This chapter provides an introduction to the Accela Pump shown in Figure 1 Figure 1 Accela Pump Contents Functional Description Specifications ...

Page 20: ...have three manual controls the power switch the purge valve and the pulse dampener flush valve To turn on the power to the unit push the power switch that is located on the front of the pump below the left door To redirect the flow path away from the outlet to the autosampler open the purge valve To prime the pulse dampener open the pulse dampener flush valve The Accela Pump has four major compone...

Page 21: ...wo sapphire pistons and their seals upper and lower piston chambers and various PEEK seal rings The inlet module contains a built in low volume mixer Four high precision proportioning valves are connected to the inlet module and four actuating coils are connected to the ends of the proportioning valves All of these components are integrated into a single mechanical hydraulic assembly which minimiz...

Page 22: ... the purge valve is open and the solvent flow is experiencing backpressure from either an LC column or a backpressure regulator mobile phase exits the LDA from the left side of the outlet module passes through the crossover tubing that connects the LDA to the pressure transducer and exits out the purge valve When the purge valve is closed mobile phase flows out the front of the LDA assembly and in...

Page 23: ...of stainless steel tubing The loop terminates with a priming valve pulse dampener flush valve When the priming valve is open you can flush or fill the dampening loop with an appropriate solvent such as methanol or isopropanol When the priming valve is closed the loop is shut off from the flow path and absorbs pump pulsations Because the 2 mL loop is shut off from the flow path it adds no gradient ...

Page 24: ...bile phases that do not require additional mixing If you have an older version of the Accela Pump a Thermo Fisher Scientific field service representative can install the dynamic mixer on site for a service charge and the cost of the Accela Pump Dynamic Mixer Upgrade Kit CAUTION Keep the pulse dampener flush valve closed during normal operation of the pump If you do accidentally open the valve whil...

Page 25: ...a system PC has been established Flashing green A pump program is downloading from the data system PC Run Amber The power switch is in the On position but the pump pistons are idle producing no flow Flashing amber A firmware download is in progress or an error condition has occurred Green The pump pistons are moving but a pump program is not running This state can occur when the pump is under dire...

Page 26: ...rnary Composition accuracy 1 at a flow rate of 25 to 500 μL min Piston volume 24 μL Delay volume 65 μL LDA 100 μL with dynamic mixer Degasser type Vacuum membrane Teflon AF Degasser capacity 4 channels Degasser volume 500 μL per channel Pulse dampener zero gradient delay volume Wetted parts 316 stainless steel titanium PEEK sapphire TZP ceramic FEP GFP ruby Remote controls Start USB interface for ...

Page 27: ...and fill it out when you have successfully completed the installation Include the completed checklist in your maintenance records Perform the installation in the sequence presented on the Installation Checklist and detailed in this chapter Contents Installation Checklist Unpacking and Inspecting the Instrument Making the Initial Instrument Preparations Making the Initial Back Panel Connections Con...

Page 28: ...ump appropriately see Making the Initial Instrument Preparations on page 12 U Make the back panel connections see Making the Initial Back Panel Connections on page 13 U Connect the solvent lines see Connecting the Low Pressure Solvent Lines on page 17 U Connect the power cord and turn on the instrument see Powering On for the First Time on page 24 U Add the pump to the instrument configuration see...

Page 29: ...ing list If any items are missing contact your Thermo Fisher Scientific service representative immediately Table 2 Accela Pump Accessory Kit Description Part number Adapter Luer type A0796 010 10 mL Luer tipped syringe 3301 0151 Tubing convoluted F5034 040 Fuses 5 20 mm 2A 250 V TL time delay 5120 0025 Cable RS232 shielded 72011 63008 Cable USB A to B 3 0 m shielded ROHS 00302 99 00014 Connector p...

Page 30: ...have a stable room temperature The area must also be free from dust moisture direct sunlight strong electromagnetic fields and physical vibrations Allow at least 15 cm 6 in of clear space between the back panel of the pump and any wall or obstruction This allowance provides access to the back panel connectors and allows sufficient room for venting the electronic components Do not turn on the pump ...

Page 31: ...rlier versions of the Accela Pump do not have a digital I O port In addition the fan vents on earlier versions of the Accela Pump are located on the bottom of the pump instead of the back panel Accela Pump 60057 60010 12472 5 653 876 6 248 157B1 61010 1 Model Serial No Part No Options Firmware This product is manufactured under and covered by one or more of the following U S patents 355 River Oaks...

Page 32: ...le The Accela Pump Accessory Kit contains a USB cable that is approximately 3 m long Y To connect the pump to the computer with the USB cable 1 Connect the series A plug of the USB cable to an available USB port on the computer Figure 7 shows the USB plugs and sockets Figure 7 USB plugs and sockets 2 Connect the series B plug of the USB cable to the USB port on the back of the Accela Pump CAUTION ...

Page 33: ...the system interconnect cable 1 Plug one of the pump connectors with a yellow PUMP sticker into the 8 pin socket on the back panel of the Accela Pump 2 Plug the autosampler connector with the A S tag on its adjacent cable into the left 8 pin socket on the back panel of the Accela Autosampler 3 If your LC system contains an Accela PDA Detector plug one of the detector connectors with a blue DETECTO...

Page 34: ...are Manual Thermo Scientific Figure 9 Schematic of the connections for the system interconnect cable RS232 1 2 3 4 5 6 7 8 USB GND 5V PUMP STOP GRAD START PUMP READY INJECT HOLD PRESSURE GND Accela Pump Accela Pump Accela Autosampler Accela PDA Detector System interconnect cable ...

Page 35: ...nd can saturate solvents inside the solvent lines that connect the solvent bottles to the degassing unit In this case purging the lines for a few minutes proves effective The solvent lines that connect the degassing unit to the proportioning valves are double walled and therefore not permeable to air The Accela system accessory kit contains 240 inches of 1 8 in OD 1 16 in ID tubing and four Super ...

Page 36: ...nd screw the cap onto the solvent reservoir bottle until it is secure 5 Position the bottle in the Accela solvent platform allowing the solvent inlet line to hang down along the left side of the Accela system Note Four Teflon end of line filters come with the Accela System Kit Solvent Interconnect Kit These filters thread onto the solvent lines Only use non metallic filters on solvent lines Note T...

Page 37: ...teel compression ring see Figure 11 The compression ring has two sides see Figure 12 When you place the three components of the fitting on the end of a solvent line the flattened side of the ring faces the nut and the angled side of the ring faces the ferrule Figure 11 Super Flangeless fitting components Figure 12 Drawing of the compression ring sides Tubing with square cut end Nut Compression rin...

Page 38: ...acing the compression ring slide the ferrule onto the end of the tubing 4 Swage the fitting onto the tubing see Figure 14 a Create a compression tool by screwing a Super Flangeless nut into one end of a 1 4 inch 28 thread internal union b Insert the tubing with the fitting assembly into the other end of the union c Hold the tubing to the bottom of the tool while tightening down the nut Figure 14 U...

Page 39: ...f fitting swaged onto tubing 5 Insert the tubing with the properly swaged fitting into inlet port A and then tighten the nut fingertight Be careful not to cross thread the fitting which can cause solvent leakage see Figure 16 6 Pass the solvent inlet line through the openings along the left side of each Accela module to complete the installation 7 Repeat the above steps for the B C and D solvent i...

Page 40: ...itting Y To connect the Accela Pump to the Accela Autosampler 1 Place the Accela Autosampler on top of the Accela Pump The Accela Pump is shipped with the LDA outlet connected to the inline filter holder inlet and the inline filter holder outlet connected to the mixer inlet You might need to reconnect the inline filter holder after performing maintenance on the liquid displacement assembly LDA 2 C...

Page 41: ...Accela Pump Hardware Manual 23 Figure 18 Connection between the pump and the autosampler PU RGE PULSE DAMPENER FLUSH Inline filter holder Mixer outlet 20 cm length of high pressure tubing 10 cm length of high pressure tubing Connection to inlet of heat exchanger LDA outlet ...

Page 42: ...it to line power 3 Push the power switch to engage it depressed position The power indicator POWER LED turns solid green 4 If the POWER LED does not illuminate see Pump Troubleshooting Guide on page 84 Call your Thermo Fisher Scientific service representative if you require assistance Before you can operate the Accela Pump first add it to the data system configuration for your instrument see Chapt...

Page 43: ...Depending on the data system version do one of the following For Xcalibur versions below 2 1 from the computer desktop choose Start All Programs Xcalibur Instrument Configuration The Instrument Configuration window appears see Figure 19 For Xcalibur 2 1 and higher from the computer desktop choose Start All Programs Thermo Foundation Instrument Configuration The Thermo Foundation Instrument Configu...

Page 44: ...em Instrument Configuration Adding the Pump to the Xcalibur Instrument Configuration 26 Accela Pump Hardware Manual Thermo Scientific Figure 19 Instrument Configuration window for Xcalibur versions lower than 2 1 ...

Page 45: ...r 2 1 or higher 2 To show all the instruments in the Device Types list select All The installed instruments appear as buttons in the Available Devices pane 3 In the Available Devices pane double click the Accela Pump button A copy of the Accela Pump button appears in the Configured Devices pane 4 In the Configured Devices pane double click the Accela Pump button The Accela Pump dialog box appears ...

Page 46: ...tive high check box see Figure 22 If you do not select these two check boxes the autosampler does not recognize the input signals from the Accela Pump and it does not begin an injection sequence when you start a sequence After you submit a run the Accela Autosampler waits for a Pump Ready signal from the pump The pump issues this signal after its pressure transducer monitors a stable backpressure ...

Page 47: ...hermo Foundation Instrument Configuration The Thermo Foundation Instrument Configuration window appears see Figure 20 on page 27 2 In the Configured Devices pane double click the Accela Autosampler button The Accela Autosampler Configuration dialog box appears 3 Click the Signal polarity tab The Signal Polarity page appears see Figure 22 4 Make sure that the Pump ready active high check box and th...

Page 48: ...he computer desktop choose Start All Programs Chromatography ChromQuest Go to step 2 For ChromQuest SI from the computer desktop choose Start All Programs Chromatography ChromQuest SI Config The ChromQuest SI Configuration dialog box appears Click Instrument Configuration The Instrument Configuration dialog box appears Go to step 4 2 To add the Accela instrument to the Enterprise a Right click The...

Page 49: ...est 4 Do the following a In the Instrument name box type a name that identifies your instrument b In the Instrument type list do one of the following To modify the configuration of an Accela system do not select an instrument type To create a new instrument select Accela A message appears warning you that this selection erases the current configuration see Figure 24 Click OK Figure 24 Warning mess...

Page 50: ...pane do the following a Double click the Accela Pump button A copy of the button appears in the Configured modules pane b Double click the Accela AS button A copy of the button appears in the Configured modules pane c Double click the Detector button The Analog button appears in the Configured modules pane see Figure 26 Figure 26 Accela dialog box with instrument added to the configuration ...

Page 51: ... c Click OK to accept the entries and close the Accela Pump Configuration dialog box 7 Configure the Accela Autosampler a In the Configured modules pane double click the Accela AS button The Accela AS Configuration dialog box appears b Make the appropriate selections and entries c Ensure that the Inject when Inject Hold signal is Off check box is selected see Figure 28 Note In ChromQuest 5 0 the A...

Page 52: ...box 8 Configure the Accela PDA Detector a Double click the Analog button in the Configured modules pane The Detector Configuration dialog box appears b In the Detector name box type an identifying name for the PDA detector c In the Detector type list select PDA In the Acquisition source list ChromQuest automatically selects PDA Plus ACQ d Click to open the PDA Plus dialog box ...

Page 53: ...tem computer f In the ID number box type an identifying number for the detector such as its serial number g Click OK to close the PDA Plus dialog box h Click OK to close the Detector Configuration dialog box 9 Click OK to close the Accela dialog box 10 For ChromQuest SI click Close in the ChromQuest SI Configuration dialog box to return to the computer desktop Note The stack value must match the u...

Page 54: ......

Page 55: ... from the solvent lines that are connected to the built in degassing unit Y To remove air from or to draw fresh solvent through a solvent line 1 For the solvent line that you are removing air from or drawing fresh solvent through A B C or D disconnect the corresponding tubing from the degasser outlet port 2 Connect a Luer Lok fitting to the degasser outlet port that you removed the tubing from see...

Page 56: ... LDA and from the solvent lines that connect the degassing unit to the proportioning valves start the solvent flow by using the data system controls see Using the Data System to Start and Stop the Solvent Flow on page 38 Using the Data System to Start and Stop the Solvent Flow You can start and stop the solvent flow by using the data system start and stop controls Using Xcalibur to Start and Stop ...

Page 57: ...up The Instrument Setup window opens 2 In the view bar click the Accela Pump button The Instrument Setup view for the Accela Pump opens 3 Choose Accela Pump Direct Control to open the Direct Control dialog box shown in Figure 30 Figure 30 Xcalibur Direct Control dialog box for the Accela Pump 4 Select the Take pump under control check box 5 Type percentages for the inlet solvent lines that you wan...

Page 58: ...rol area of the Instrument Status page for the Accela Pump Y To start and stop the solvent flow by using the ChromQuest data system 1 From the Main Menu window double click the icon for your instrument The online Instrument window opens 2 Choose Control Instrument Status The Instrument Status window appears with the Accela Pump page displayed see Figure 31 Use the Accela Pump page to view the pump...

Page 59: ...pump and periodically thereafter Y To prime the pulse dampener 1 Fill a solvent reservoir with particulate free HPLC grade methanol 2 Connect an HPLC column or a flow restrictor to the LDA outlet 3 Insert the tip of the 10 mL syringe into the tubing that is connected to the pulse dampener flush valve and then open the valve by turning it counterclockwise 4 Set the flow rate to 1000 μL min 5 Fill t...

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Page 61: ...inimizing Pump Pulsation on page 55 If you cannot reduce the variation in backpressure by performing these procedures disassemble and clean the LDA and replace the primary piston seals see Replacing the Primary Pistons Seals on page 67 The following topics describe how to monitor the pump pulsation from the data system Using Xcalibur to Monitor the Pump Status Using ChromQuest to Monitor the Pump ...

Page 62: ...window If you cannot see the Information view it has been turned off Y To open the Info view and check the status of the Accela Pump 1 From the Home Page window choose View Info View to turn the Information view on and off 2 To display the Status page click the Status tab 3 To display the status pages for the Accela Pump click the Accela Pump listing in the directory tree Check the value for the s...

Page 63: ...but is producing a zero flow rate Homing The pistons are moving to the home position At lower flow rates the pistons take longer to reach the home position Ready The pump is waiting for the start signal Running Isocratic The pump is under direct control and pumping an isocratic mobile phase or running a method that contains a one line pump program Running Gradient The pump is running a method that...

Page 64: ...iked methanol for this procedure The absorbance reading for the 100 spiked methanol solution should be in the 600 to 800 mAU range If your detector contains a standard 10 mm LightPipe flow cell prepare the spiked methanol solution by adding 7 mg of ethyl paraben Pump Test Mix P N A4904 010 to 1 L of HPLC grade methanol If your detector contains a 50 mm LightPipe flow cell prepare the spiked methan...

Page 65: ...nol B spiked methanol Steps 10 min hold 20 increments Detection UV 254 nm The gradient program described in Table 4 should create a gradient profile containing five steps of equal height as shown in Figure 34 Inaccuracies in the step heights indicate proportioning errors Table 4 Gradient program Time min A B 0 00 100 0 0 0 10 0 100 0 0 0 10 01 80 0 20 0 20 0 80 0 20 0 20 01 60 0 40 0 30 0 60 0 40 ...

Page 66: ...er than 6 mAU for any of the steps would be a failing result If the pump passes both the pulsation and proportioning tests it is working properly If the pump fails the pulsation test or the proportioning test try to restore proper performance by cleaning the proportioning valves as described in Cleaning the LDA Components without Disassembling the LDA on page 56 If this fails to restore proper pum...

Page 67: ...insulated red PEEK 1 16 in OD 0 005 in ID b Prepare a spiked methanol solution as described in Testing the Pump Proportioning on page 46 c Fill one solvent reservoir bottle with 100 methanol d Fill a second solvent reservoir bottle with the spiked methanol solution e Purge the solvent lines to replace the old solvents with the new solvents 2 Create a data acquisition method Use the following detec...

Page 68: ...ile For Xcalibur create a one line sequence containing the instrument method and then run the sequence For ChromQuest click the Single Run button Enter the appropriate information in the Single Run dialog box and then start the run 7 Calculate the gradient delay volume of the pump a To determine the total gradient delay volume multiply the gradient delay time by the flow rate b To determine the gr...

Page 69: ...Pump Thermo Scientific Accela Pump Hardware Manual 51 Figure 35 Graphical display of the baseline shift that occurs as the spiked mobile phase reaches the detector Solvent proportioning valve to detector Absorbance mAU Inject T1 Time min Baseline shift ...

Page 70: ......

Page 71: ...ry piston seals the check valve film seals in the top and bottom of the displacement chambers and the outlet film seal might also require replacement If you detect a leak from the LDA remove the LDA from the pump disassemble the LDA clean its components and replace the primary piston seals CAUTION Do not disassemble the dynamic mixer It does not contain serviceable parts Contents Flushing the Pist...

Page 72: ...recipitate can scratch the piston and shorten the life of the piston seals To lengthen the life of the primary piston seals and the pistons always rinse the piston guide bushings with distilled water after pumping buffered solutions see Figure 36 The piston guide bushings come with two pieces of standard 1 8 in 1 16 in PTFE tubing Y To flush the piston guide bushings 1 If the outer piece of PTFE t...

Page 73: ...apped in the piston chambers causes an excessive variation in the backpressure Y To purge air out of the piston chambers 1 Open the purge valve by turning its wingnut counterclockwise see Figure 37 2 Insert the 10 mL syringe which is supplied in the accessory kit into the tubing attached to the purge valve 3 Pump 100 methanol or 100 acetonitrile through the liquid displacement assembly LDA at a fl...

Page 74: ...urge valve by turning the wingnut to the left side of the LDA counterclockwise see Figure 37 2 Set up the system to pump the same solvent on all four channels by connecting all four solvent reservoir lines to one solvent bottle 3 Attach a 10 mL disposable syringe to the tubing attached to the purge valve The syringe is supplied in the Accela Pump Accessory Kit see Accela Pump Accessory Kit on page...

Page 75: ...p an additional 6 mL of water through the LDA 5 Sequentially pump solvents of increasing polarity through the LDA The last flush solvent should be miscible with the mobile phase that you plan to use 6 Close the purge valve 7 Retest the pressure variation by returning to the initial conditions that displayed the problem Make sure that the backpressure at these conditions is at least 40 bar Table 6 ...

Page 76: ... Chambers and the Inlet Module For instructions on disassembling the LDA see Disassembling the LDA on page 64 Cleaning the Check Valves To clean the check valves Thermo Fisher Scientific recommends using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit Y To clean the check valves Using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit gently flush...

Page 77: ...ing a needle graphite pencil or equivalent tool carefully remove the PEEK film seals see Figure 38 Be careful not to scratch the displacement chambers as you remove the film seals as this might cause leaking Figure 38 View of the PEEK film seal inside a displacement chamber 3 Place the displacement chambers and the inlet module in an ultrasonic bath First use water to remove any buffer precipitate...

Page 78: ...he pump 1 Turn off the power to the pump 2 Raise the solvent filter in each bottle above the solvent level so that only the fluid in the lines drains out Use a beaker to collect the mobile phase contained in the lines 3 Using an 8 mm open ended wrench disconnect the crossover tubing from the pulse dampener see Figure 39 4 Using a 1 4 in open ended wrench disconnect the stainless steel tubing from ...

Page 79: ...d then pull the actuating coils off the ends of the proportioning valves see Figure 40 Figure 40 View of the actuating coils with their nylon screws A B C D PU RGE PULSE DAMPENER FLUSH Crossover tubing Connection to outlet module Captive screws 3x CAUTION Take care not to pinch the wires that connect the actuating coils to the pump Pinching a connecting wire can irreversibly damage the thin electr...

Page 80: ... cover to the pump see Figure 41 Figure 41 Removing the drive arm cover 8 Using a 4 mm L hex wrench turn the two clamping screws 3 to 4 turns to loosen the piston spring clamps see Figure 42 Figure 42 Loosening the clamping screws 9 Remove the two piston spring clamps as shown Figure 43 Figure 43 Removing the piston spring clamps retaining clips Drive arm cover M3 screws ...

Page 81: ...the front panel of the pump see Figure 39 on page 61 12 Pull the LDA forward until it clears the two mounting pins and then lift it up approximately 1 cm Figure 45 shows the two mounting pins Figure 45 Pulling the LDA off the mounting pins Piston heads CAUTION Take care not to pinch the wires that connect the actuating coils to the pump Pinching a connecting wire can irreversibly damage the thin e...

Page 82: ...g the Holding Hooks Bushings and Pistons from the LDA Body 2 Removing the Stacked Components from the LDA Body Removing the Holding Hooks Bushings and Pistons from the LDA Body Y To remove the holding hooks bushings and pistons from the LDA Body 1 Using the 4 mm L Hex wrench loosen the fastening screws on the left side of the LDA as shown in Figure 46 These screws secure the holding hooks to the b...

Page 83: ...ng the holding hooks bushings and pistons from the LDA body If you are replacing the primary piston seals but do not need to clean the stacked components of the LDA go to Replacing the Primary Pistons Seals on page 67 You can remove the primary piston seals from the displacement chambers without removing the displacement chambers from the LDA body Guide bushing Piston Holding hook ...

Page 84: ...nch loosen the top set screw and then slide the LDA compression lid out see Figure 48 Figure 48 Removing the compression lid from the LDA 2 Remove the inner components of the LDA body These components include the outlet module the upper displacement chamber and the lower displacement chamber with check valves Figure 49 shows the stacked components of the current LDA For information on the previous...

Page 85: ...m the LDA body see Removing the Holding Hooks Bushings and Pistons from the LDA Body on page 64 4 Remove the used primary piston seals see Figure 52 on page 69 a Carefully insert a piston into the hole in the center of the seal but do not push the piston further into the bore of the displacement chamber b Gently pry the seal out of the bore 5 Carefully slide the piston through the bore in the hold...

Page 86: ...al installed on the piston 6 Slide the guide bushing onto the piston see Figure 51 Figure 51 View of the holding hook piston secondary piston seal and guide bushing assembly 7 With its spring facing toward you slide the black primary piston seal onto the piston The piston should protrude by approximately 6 mm as shown in Figure 52 Holding hook Secondary piston seal Piston Guide bushing ...

Page 87: ...embly 8 Finish reassembling the LDA If you did not remove the stacked components from the LDA go to Installing the Holding Hooks and the Piston Assemblies on page 73 If you removed the stacked components from the LDA go to Inserting the Components of the LDA Stack into the LDA Body on page 71 6 mm Guide bushing Primary piston seal ...

Page 88: ...he LDA Body 4 Installing the Holding Hooks and the Piston Assemblies Connecting the Quaternary Inlet Module to the LDA Body Y To connect the quaternary inlet module to the LDA body 1 Insert the inlet blind cartridge into the quaternary inlet module see Figure 53 Figure 53 Inserting the inlet blind cartridge 2 Slide the top rim of the quaternary inlet module into the bracket on the bottom of the LD...

Page 89: ...heck valve assembly Inserting the Components of the LDA Stack into the LDA Body If they are installed correctly the inner components of the LDA form a perfect stack that ensures smooth movement of the pistons Y To install the inner components of the LDA stack 1 Because the fit between the check valves and the lower displacement chamber is loose place the LDA body on a flat surface and then insert ...

Page 90: ... 56 Installing the stacked components of the LDA 4 Slide the compression lid into the upper bracket of the LDA body see Figure 57 Figure 57 Sliding the compression lid into the LDA body Lower displacement chamber with check valves Upper displacement chamber Outlet module PEEK sealing rings LDA body Compression lid ...

Page 91: ... the compression lid Installing the Holding Hooks and the Piston Assemblies Y To install the holding hooks and piston assemblies 1 Slide each holding hook and piston unit into its designated slot see Figure 59 Figure 59 Sliding the holding hook and piston assembly into the LDA body Note For instructions on assembling the holding hook and piston assembly see Replacing the Primary Pistons Seals on p...

Page 92: ...ee Figure 60 The piston acts as a guiding aid as you tighten the fastening screws in step 3 Figure 60 View of the piston pushed into the displacement chamber 3 Using a 4 mm L hex wrench tighten the fastening screws see Figure 61 Figure 61 Tightening the fastening screws 4 Readjust the guide bushing flush adaptors and then reattach the wash tubing see Figure 62 Fastening screws ...

Page 93: ...o the front panel of the pump 1 Taking care not to pinch the wires that connect the coils to the pump mount the LDA onto the front panel of the pump The two guide pins on the pump align with the two alignment holes in the LDA body see Figure 63 Wash tubing CAUTION Although the damage is not visible pinching one of these connecting wires can irreversibly damage the internal copper wire which conduc...

Page 94: ...LDA to the pump 3 Push the heads of the pistons toward the arms of the drive mechanism leaving a gap between the piston head and the brackets at the ends of the arms see Figure 64 Figure 64 Pushing the piston heads towards the drive mechanism arms 4 Insert the two piston retaining clips between the piston heads and the brackets of the drive mechanism arms Alignment pins Drive arm mechanism Piston ...

Page 95: ...our pump is functioning properly turn on the solvent flow check for leaks and perform the pump pulsation and proportioning tests described in Chapter 5 Diagnostics Servicing the Proportioning Valves Each proportioning valve of the Accela Pump contains an electromechanically actuated ball and seat valve The ruby ball portion of the valve sits on one end of a magnetic piston that floats within the b...

Page 96: ...proportioning valve with the proportioning valve tool supplied in the accessory kit This tool fits through the solvent inlet in the end of the valve face see Figure 66 Y To dislodge the frozen valve inside the proportioning valve Push the tool which is a piece of stainless steel wire into the valve until you contact the piston and then gently push the piston away from the valve face Figure 66 Prop...

Page 97: ...d an abnormally low system backpressure To you determine that the solvent reservoir filter is clogged remove the filter from the solvent line and monitor the system backpressure as the pump draws solvent into the solvent line If the pressure trace is normal in the absence of the solvent reservoir filter replace the filter Thermo Fisher Scientific offers two solvent reservoir filters a 10 micron po...

Page 98: ...he fuse holder 3 Inspect each fuse for a burned or broken wire which indicates a blown fuse 4 Pull the blown fuse out of the holder and discard it Place the new fuse 2 0 amp 250 V TL 5 20 mm into the holder with the metal end visible 5 Snap the fuse holder back into place 6 Reconnect the power cord and turn on the pump If the replacement fuse fails in a short time you have a serious electrical pro...

Page 99: ...ve Troubleshooting Tips Begin the diagnosis of the problem with your Accela Pump by eliminating all other possible sources of trouble Before you invest time trying to diagnose a suspected pump problem you should verify that the pump is the only source of difficulty Systematically eliminate all other instruments in your Accela LC system as the source of trouble If you are not sure which component o...

Page 100: ...ting Guide on page 84 Y Unstable baseline or drift 1 System column is not equilibrated allow more equilibration time 2 Refer to the troubleshooting guide for your detector 3 See problem 7 on page 85 Y Baseline noise 1 Check for air bubbles in the system 2 Check for system solvent contamination 3 The pulse dampener is contaminated prime the pulse dampener Y No peaks 1 Check the detector and data sy...

Page 101: ...r retention time reproducibility 1 Check the system for leaks and bubbles 2 System column is not equilibrated and more time should be allowed 3 Check the column performance 4 Refer to the troubleshooting guide for your detector 5 See problem 7 on page page 85 Y Poor peak area reproducibility 1 Check the column performance 2 Refer to the troubleshooting guide for your autosampler Y No instrument or...

Page 102: ...or b Contact Thermo Fisher Scientific service representative c Disconnected motor cable c Contact Thermo Fisher Scientific service representative d Defective drive circuit d Contact Thermo Fisher Scientific service representative e Blown internal fuse e Contact Thermo Fisher Scientific service representative 4 Incorrect solvent composition a Dirty proportioning valve a Flush the valve with the app...

Page 103: ...isassembling the LDA on page 56 or replace the proportioning valves d Dirty check valve d Flush the LDA with the appropriate solvents see Cleaning the LDA Components without Disassembling the LDA on page 56 or replace the check valves e Leaking piston seal e Replace the piston seals see Replacing the Primary Pistons Seals on page 67 f Clogged solvent reservoir filter f Replace the filter see Repla...

Page 104: ...nual Thermo Scientific Y To reconnect the loose LED cable 1 Using a 2 Phillips screwdriver remove the screw that secures the LED cover to the inner left door and then remove the cover 2 Reconnect the LED cable to the board 3 Secure the LED cover to the door Screw LED cable ...

Page 105: ...rd deviation value of 1 0 percent or less is acceptable Abnormal Pressure Pulsation Abnormal pressure pulsations can normally be divided into two categories Large pressure pulsations are generally considered to be those with a standard deviation in pressure of greater than 10 bar 1 MPa 147 psi Minor pressure pulsations are those with a standard deviation in pressure of less than 10 bar Pressure Pu...

Page 106: ...igh pressure When your LC system is set up to operate at a backpressure below 7000 psi 483 bar make sure that you select the low pressure operating mode 0 to 7000 psi for your instrument control method Under extreme conditions flow rates of less than 100 μL min and with a low backpressure less than 40 bar you might observe a slight instability in the detector baseline at high sensitivity This prob...

Page 107: ...t reservoir A line from the inlet port of the degasser and verify unrestricted flow of solvent through the tubing 2 If the solvent flow is normal reconnect the line to the inlet port of the degasser and disconnect the corresponding degasser outlet line 3 Check for unrestricted flow of solvent through the degassing chamber The flow out of the degasser is normally slower than that going to the degas...

Page 108: ...ning the LDA Components without Disassembling the LDA on page 56 Irreproducible Retention Times There are many reasons for retention time problems Some of the more common causes are as follows An old or damaged column Inconsistent stationary phase Was a new column installed A validated method that lacks robustness Changes in mobile phase composition Temperature fluctuations Pump problems If you su...

Page 109: ...ontains a list of replaceable parts and their corresponding part numbers To place an order contact your local Thermo Fisher Scientific service representative Contents Liquid Displacement Assembly LDA Parts Accela Pump Accessory Kit Accela System Kit Solvent Interconnect Kit Other Parts and Assemblies ...

Page 110: ...0950 01 00124 Ti 2 4 Seal PEEK film Accela Pump 00950 01 10013 PEEK 3 1 Chamber upper displacement Accela Pump 00950 01 00123 Ti 4 2 Seal high pressure GFP Accela Pump 00950 01 00129 PTFE GFP Ti 5 1 Check valve outlet Outlet holder check valve cartridge and PEEK seal for the Accela Pump 00950 01 00274 Ti ruby sapphire 6 1 Chamber lower displacement Accela Pump 00950 01 00121 Ti 7 1 Check valve inl...

Page 111: ...e 69 Liquid displacement assembly original version parts 1 to 8 1 Outlet module 2 PEEK film seal 4 Primary piston seal Fluoroloy 907 4 Primary piston seal Fluoroloy 907 5 Outlet holder and check valve 7 Inlet holder and check valve 8 Inlet blind cartridge 3 Upper displacement chamber 6 Lower displacement chamber 2 PEEK film seal ...

Page 112: ...70 Liquid displacement assembly current version parts 1 to 8 1 Outlet module 4 Primary piston seal Fluoroloy 907 4 Primary piston seal Fluoroloy 907 5 Check valve and PEEK film seals 7 Check valve and PEEK film seals 8 Inlet blind cartridge 3 Upper displacement chamber 6 Lower displacement chamber 2 PEEK film seal 2 PEEK film seal ...

Page 113: ...a Pump Hardware Manual 95 Figure 71 Liquid displacement assembly parts 9 to 17 LDA body 14 Secondary piston seal 10 Guide bushing Holding hook 12 Piston sapphire 15 Actuating coil 17 Proportioning valve 16 Proportioning valve seal ring 13 Nylon set screw 11 Quaternary inlet module 9 Compression lid ...

Page 114: ...1 Pressure sensor and pulse dampener unit 00950 01 00112 Ti 316 stainless steel 19 2 Wing screw for purge valve 00950 01 00117 Ti 20 2 Purge valve spindle 00950 01 00118 Ti Ruby 21 1 Crossover tubing 00950 01 00113 Ti 22 2 Z arm screw set 00950 01 00115 Ti 23 2 Piston spring clips 00201 11328 Stainless steel 19 Wing screw for purge valve 20 Purge valve spindle 23 Piston spring clips 21 Crossover t...

Page 115: ...nch 1 4 in and 5 16 in 5401 0400 Wrench L hex 4 mm 00725 00034 Wrench open end 8 10 mm 00950 30005 Accela System Kit The Accela System Kit P N 60057 60060 contains the cables tubing fittings and so on used to interconnect the system modules Ethernet switch 5 port 10T 100TX 100 240V 00025 01 00024 Cable Ethernet patch with two ferrites 97355 98006 Cable Surveyor Plus LC MS interconnect 60053 63034 ...

Page 116: ...sembly flexible LED internal 60157 63006 Cable assembly degasser LED 60157 63008 Cable assembly COMM and gradient LED 60157 63010 Cable assembly RS232 internal 60157 63012 Cable assembly AC power internal 60157 63014 Cable assembly interface UHPLC 60157 63018 Cable motor index 00950 01 00001 Cable USB 2 0 A to B 14 in UL 00302 99 00009 Compression nut 1 16 in 10 32 stainless steel 2522 1880 Filter...

Page 117: ... pressure tubing and fittings tubing and fittings that connect the degassing unit outlet ports and the proportioning valves 00950 01 00021 Tubing stainless steel 5 cm length 0 005 in ID 00301 01 00017 Tubing stainless steel 10 cm length 0 005 in ID 00301 01 00008 Tubing stainless steel 20 cm length 0 005 in ID 00301 01 00009 Vacuum degas assembly 00108 01 00011 ...

Page 118: ......

Page 119: ...ntegrated dynamic mixer contains check valves that do not screw into the lower displacement chamber The current check valves are compatible with all versions of the pump Installing the check valves in the original version of the LDA requires the use of adapter holders Depending on the check valve type follow one of these procedures Installing the Check Valves and Holders Installing the Screw In Ch...

Page 120: ...acement chamber bores is loose place the LDA on a flat surface as you insert the lower displacement chamber and check valve assembly Installing the Screw In Check Valves discontinued version Y To install the check valves that screw into the lower displacement chamber 1 Install the PEEK film seals and check valves into the lower displacement chamber as shown in Figure 74 a Insert a film seal into t...

Page 121: ...alves 2 Insert the lower displacement chamber into the LDA body just far enough so that the body serves as a vice and then tighten the check valve fitting with an 8 mm open ended wrench see Figure 75 3 Remove the displacement chamber from the LDA body Rotate it and repeat step 2 Figure 75 Tightening the outlet check valve fitting Inlet check valve Outlet check valve PEEK film seals Lower displacem...

Page 122: ...the lower displacement chamber is loose place the LDA on a flat surface and then insert the lower displacement chamber and check valve assembly The orientation arrow on the lower displacement chamber points away from the LDA body see Figure 76 2 Install the upper displacement chamber and two PEEK film seals 3 Install the outlet module Figure 76 Installing the stacked components of the LDA 4 Slide ...

Page 123: ...cela Pump Installing the Components of the Discontinued LDA Stack Thermo Scientific Accela Pump Hardware Manual 105 5 Using a 4 mm L hex wrench tighten the compression lid as shown in Figure 78 Figure 78 Tightening the compression lid ...

Page 124: ......

Page 125: ...ws tightening 76 channel degasser volume 3 check valves blocked 90 cleaning 56 58 contaminated 43 55 85 damaging 58 description 3 discontinued 102 installing in discontinued LDA 102 replacing 64 checklist 10 ChromQuest data system configuring an instrument 30 configuring the Accela Pump 33 instrument status page for the Accela Pump 45 starting and stopping the solvent flow from the pump 40 clampin...

Page 126: ...y volume guide bushing installing 68 H Handle degasser feedback check box ChromQuest 33 heat exchanger autosampler 22 high pressure fitting 22 holding hooks installing 73 removing 65 I inlet blind cartridge installing 70 installation checklist 10 connecting the pump to the autosampler 22 low pressure solvent lines 17 serial communication cable 14 system interconnect cable 15 unpacking 11 interconn...

Page 127: ...eaks 83 motor will not run 84 powering on 24 specifications 8 pump program downloading 7 pump pulsation abnormal 87 causes of excessive 43 55 damaged piston seals 87 defined 87 dirty check valves 88 dirty solvent reservoir filters 87 effect of trapped air 43 55 monitoring 43 normal 87 test failure 48 Q quaternary pump functional description 2 R records maintenance 9 regulatory compliance iii retai...

Page 128: ...e baseline 82 Troubleshooting Guide 84 tubing connecting pump to autosampler 97 connecting the pump to the autosampler 22 low pressure solvent lines 17 97 U unpacking your pump 11 upper displacement chamber installing 71 104 USB cable 14 V vacuum degasser description 3 LED 7 specifications 8 valves check See check valves proportioning See proportioning valves pulse dampener flush 5 purge 4 W wires...

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