1
Introduction
Functional Description
Thermo Scientific
Accela Pump Hardware Manual
5
Pulse Dampening Assembly
The pulse dampening assembly is located behind the front panel of the pump to the left of the
LDA (see
Figure 4
).
Figure 4.
Pulse dampening assembly (located behind the front panel to the left of the LDA)
The pulse dampening assembly consists of a low volume T-connector that the mobile phase
passes through. Attached to the side leg of the T-connector is a 2 mL loop of stainless steel
tubing.
The loop terminates with a priming valve (pulse dampener flush valve). When the priming
valve is open, you can flush or fill the dampening loop with an appropriate solvent such as
methanol or isopropanol. When the priming valve is closed, the loop is shut off from the flow
path and absorbs pump pulsations. Because the 2 mL loop is shut off from the flow path, it
adds no gradient delay volume to the LC system.
The priming solvent in the loop does not interfere with the purity of the mobile phase, and its
composition does not need to match the mobile phase.
Pulse
dampening
coil
Pressure
sensor
cable
Pulse dampener
flush valve
Pressure sensor
Purge valve
