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DecalMATE

MM112-003 - Operator Manual

MILESTONE SUGGESTED RECOVERY PROTOCOL FOR AIR DRIED SAMPLES

1. Running tap water for 30 minutes.

2. Place in formol-glycerol solution (**) until tissues become wet. Tissues may remain in formol-glycerol

for up to 8 hours without adverse results.

3. Reprocess on Fixation/Decalcification system in usual manner.

(**)Formol-Glycerol Working Solution:

Formalin- Sodium Acetate (Stock)

90ml

Glycerin (Gyicerol)

10ml

Summary of Contents for DecalMATE

Page 1: ...DecalMATE Automatic Fixation Decalcification System OperatorManual MM112...

Page 2: ......

Page 3: ...carefully this operator manual and to keep it in reach for convenient and fast consulting For any possible clarification or any request for assistance please contact either our Representative in your...

Page 4: ...g out 22 2 9 1 Create a new user 23 2 10 Set system date and time 24 2 11 Tanks 25 2 11 1 Labels 25 2 12 Load DecalMATE with reagents 26 2 12 1 Load DecalMATE with reagents 26 2 13 Most used programs...

Page 5: ...m the terminal to the USB key 64 7 MAINTENANCE 66 7 1 Introduction 66 7 2 After each run 66 7 2 1 Rack cleaning procedure 67 7 3 Daily 67 7 4 Twice a week 67 7 5 Weekly 67 7 6 Yearly 67 8 OPTIONAL 68...

Page 6: ...caution against electric shock Be sure to follow the instruction to prevent accidents resulting from electric shock Biohazard be careful when you execute the procedure marked with this symbol danger...

Page 7: ...rocesses for histopathological applications Fixation Decalcification The Milestone DecalMATE must be operated exclusively with the reagents listed in section Compatible reagents chapter 1 7 1 3 Techni...

Page 8: ...storage 4GB 1CPU 1GHz Windows Embedded Compact 7 Programs Multiple pre stored programs with customizable user defined programs I O Interface 2x USB port 2 0 1x LAN Ethernet 10 100 Mbps RJ 45 1 4 Trans...

Page 9: ...truction in the documentation might lead to a reduction of device safety If the equipment is not working properly please contact your supplier or the manufacturer Do not remove the instrument accessor...

Page 10: ...atives Decalcification Other Milestone FineFIX Ethylenediaminetetraacetic acid EDTA 20 Water Formalin buffered or unbuffered Formic acid 20 Hydrochloric acid 10 Nitric acid 7 Trichloroacetic acid 10 A...

Page 11: ...aged of equipment is mandatory It is necessary to return the used equipment to the distributor or to inquire about the presence of a local empowered system for collection and disposal of WEEE The in o...

Page 12: ...rovide a bench of at least 82cm 32in in width and 114cm 45in in depth and that can stand a weight of 70kg 154lb DecalMATE FULL weight all reagents loaded in proper tanks Then place DecalMATE on the be...

Page 13: ...mensions Width 420mm 16 5in Height 740mm 29in Depth 820mm 32in 940mm 37in with fumes tube Space dimensions required to locate the unit Width 600mm 24in Height 840mm 33in Depth 870mm 34in 990mm 39in wi...

Page 14: ...npacking of DecalMATE to avoid any scratch or damage of the external coating The DecalMATE unit CODE 101002 230V or 101004 115V with power supply cable including the following items CODE DESCRIPTION Q...

Page 15: ...E MM112 003 Operator Manual 15 67854 Rack 30 cassettes 1 65798F Spare tank without tube 2 65628 Polypropylene Funnel for loading of reagents 1 DecalMATE Operation Manual MM112 with Maintenance Instruc...

Page 16: ...cover flip up the cover To close the CAVITY cover place white cover lid over the cavity aligning the BLUE ARROW HEAD etching on the cover with the external linear marker Magnetic safety switch for un...

Page 17: ...eter 60mm 2 36 Insert the exhaust tube on the proper outlet back panel Use the metal cable clamp to fix the hose to the exhaust fume pipeline Now connect the other side of tube to the fume extractor o...

Page 18: ...ompliant with the label positioned on the back side of our units Plug socket is visible during the normal use and easily accessible Power line protection 115V Miniature circuit breaker curve C 16A int...

Page 19: ...te the S N Serial Number of the unit to Customer Support As separable power cord it is strictly required to use power cord with wire size at least of 1 5mm equal to minimum AWG12 A size less of 1 5mm...

Page 20: ...500W minimum Output 230V 10 50 60 1Hz Transfer time 6ms max With cord set for connections Space 405 205x145mm Weight 10kg 22lb UPS unit at 115V 60Hz must be provided locally with the following minimum...

Page 21: ...IEC320 C14 blue B Finally connect the socket to main power supply connector input 3 now it is possible to turn DecalMATE ON 2 7 Remote alarm connection DecalMATE is also provided with two auxiliary o...

Page 22: ...default the main functions grouped by different colours In whatever screen you are press the Home button to return to the HOME PAGE You can customize the HOME PAGE under the USERS settings choosing be...

Page 23: ...n Add a keyboard appears Enter the NEW USER name and press Enter Now select and configure the parameters of the NEW USER choosing the USER CATEGORY USER PASSWORD click on the password box a num pad ap...

Page 24: ...recognizes through a password system who is performing some operations during the reagent management who replaces the reagents manually or moves them through the Moving a reagent function 2 10 Set sy...

Page 25: ...tone recommends a fill of at least 2L 2 11 1 Labels Every tank has double labelling 1 FILLING LABEL to be used as reference when filling the tank with reagent horizontal position 2 WORKING LABEL to be...

Page 26: ...them as follows TO DECALCIFY 2 12 1 Load DecalMATE with reagents Select Reagents from the MAIN FUNCTIONS screen or press Reagents status icon on the HOME PAGE STATUS BAR figure below left The REAGENTS...

Page 27: ...ase as follows A Unscrew the black cap B Remove the pipe C Put the tank in the filling position horizontal D Place the funnel E Fill the tank making sure the level REACHES THE MAXIMUM MAX MARK filling...

Page 28: ...re to fill all other tanks 4 If a tank is not loaded with reagent DO NOT FILL THE TANK 5 When loading of reagents needed is completed and tanks repositioned close the tanks doors 6 Now the unit is rea...

Page 29: ...rting FAVOURITE programs in the HOME PAGE 1 Press Settings from the MAIN FUNCTIONS screen and select Set favourites 2 Click on NOT DEFINED icon 3 Select the PROTOCOL the PROGRAM and the BUTTON COLOUR...

Page 30: ...ges 5 The program icon is now listed in the Choose the Favourite to Set screen 6 If USER HOME PAGE is set as FAVOURITES figure below left press to return to the HOME PAGE If HOME PAGE is set as MAIN F...

Page 31: ...on the base of the cycles run and the reagents status icon turns from green to yellow see chapter 4 Reagent Days Alert Status this number indicates that the reagent status is near expiration on the ba...

Page 32: ...ticked software requires user to enter the Run ID before running a program Enable or disable Run ID check according to your internal laboratory practice Good Laboratory Practice GLP VOLUME Select Volu...

Page 33: ...emperature in the set times by automatically adjusting the power level The following sections show how to run and modify the program values with the DecalMATE software Use the touch screen to select t...

Page 34: ...it the Run ID of the process if required then press Enter 2 Enter the number of cassettes to be processed see the figure below up to 30 then press Enter 3 Open the transparent Perspex cover and remove...

Page 35: ...lace white cover lid over the cavity aligning the BLUE ARROW HEAD etching on the cover with the external linear marker Magnetic safety switch for unit to function 6 Close the Perspex transparent cover...

Page 36: ...l check status option 10 Choose starting by selecting GO or GO DELAY for details see chapter 3 2 6 11 The RUN SUMMARY screen appears showing different information according to parameters previously se...

Page 37: ...the samples on the blue plastic area see chapter 3 2 3 select Run completed Decalcification completed then the process will end and you can proceed with the discharge of the reagent reagent Unload or...

Page 38: ...d At the end of Water rinsing nr 6 you can select Run completed If the Check status is not done immediately the samples are kept in Water rinsing 2 up to their control This period is identified with t...

Page 39: ...rack into the cavity MAKE SURE that the smaller white disk is the lastly inserted on the rack spindle before inserting the cassette rack into the cavity 6 Place white cover lid over the cavity alignin...

Page 40: ...only during the decalcification phase to check the status of the samples If you wish to disable audible notifications disable the interval check status option 12 Choose starting by selecting GO or GO...

Page 41: ...ttention must be paid to the level of reagent inside the tanks on completion of each process to avoid low reagent situation when starting a new program At the end of the process a beep sounds and the...

Page 42: ...ssible to repeat this operation until 5 times At every restart of decalcification the name of the phase will change as Decalcification nr 2 Water rinsing 2 Nr 2 decalcification nr 3 Water rinsing 2 nr...

Page 43: ...of decalcification as set at the start 1 Open the clear Perspex cover 2 Remove the white cavity cover and place it in the removable cut out slot towards the rear of the work area on the right Position...

Page 44: ...ault time Place the rack with cassettes back into the chamber with decal solution with the small white disk in position Place the white chamber cover back on in alignment with the BLUE ARROW Head and...

Page 45: ...ck status you can enter values from 5 minutes up to 3 hours 59 minutes 3 2 5 Run a program using the STEP START function In the starting screen appears the Run Summary where all process details are li...

Page 46: ...processing as set by USER During the PRE soaking you can add cassettes by clicking the Add extra cassettes button Particular attention must be paid to the level of reagent inside the tanks on complet...

Page 47: ...hs of the highlighted file Click the icons figure below to see the graphs of the different saved processing PHASES Heated phases graphs show up to 2 coloured lines describing the PRESET TEMPERATURE bl...

Page 48: ...vance status ok Run extended Press the icon to go back to the Saved processing screen 3 3 1 How to delete a saved process Select a Date and press Delete to cancel all processes saved that day left fig...

Page 49: ...AGENT PROGRAM just modified your protocol is saved there DEFAULT PROTOCOLS the Owner is MILESTONE and USER can only COPY them USER PROTOCOLS the Owner is USER and he can MODIFY DELETE and COPY them if...

Page 50: ...DecalMATE MM112 003 Operator Manual 50 1 2 3 4 5 6 7 8 9 10...

Page 51: ...ter 4 1 1 can be checked by 1 Expiration Date this field automatically shows the reagent EXPIRING DATE according to requirements set into the Reagent List green box figure above 2 Performed cycles thi...

Page 52: ...he system automatically warns before the process starts To add a new reagent or Delete it press on the relative icon see button on the left of the previous image Default reagents cannot be deleted Eve...

Page 53: ...er 2 14 Example if reagent cassettes alert status is set at 30 and a reagent checked for the processed cassettes has a limit of 180 the REAGENTS STATUS icon is GREEN until 149 cassettes one full rack...

Page 54: ...T REPLACEMENT PROCEDURE Follow the steps written below Handle reagent with care and store in a cool dry space using a tightly closed container Vapours may collect in empty containers Treat empty conta...

Page 55: ...troduce the funnel C Fill the tank making sure the level REACHES THE MAXIMUM MAX MARK filling label D Reinsert the pipe inside the tank E Screw the black cap F Put the tank in the working position ver...

Page 56: ...atus icon also turns from red to GREEN figure below Make sure tank fitting is correctly inserted in its relative housing and that the tank is aligned with the others At the end of the procedure it is...

Page 57: ...hapters 2 7 In case of power failure these functions are interrupted Reagent heating Tissue processing The terminal and the built in stirrers remain operative in order to put the samples in the safety...

Page 58: ...POWER WITH UPS The system automatically puts the specimens rack in Safe mode according to the following schedule PHASE CONDITION POWER FAILURE PROCEDURE PHASE1 POWER FAILURE PROCEDURE PHASE2 POWER FA...

Page 59: ...can continue the processing with the STEP START function starting from the phase not executed see chapter 3 2 4 for details AFTER THE 20 MINUTES If Reagent Loading Time Out occurs when nobody is in t...

Page 60: ...he terminal to the USB key Insert the USB memory stick in one of the slots placed on the right side Select Log Book button from the MAIN FUNCTIONS screen then Autosaved programs The following screen a...

Page 61: ...ry stick on the DecalMATE terminal where all processes are stored and export them on the key as described in chapter 6 1 Open the LogViewer software go to Start Menu All programs Milestone srl Milesto...

Page 62: ...rop down menu Print summary this print preview includes all phases without graphs with process and unit information Print current phase this print preview includes phase selected with process and unit...

Page 63: ...nformation TO customersupport milestonemedsrl com OBJECT Milestone UNIT NAME Serial number TEXT To Milestone Customer Support Just send the e mail 6 2 4 Change settings Select Settings icon green circ...

Page 64: ...Log Book button from the MAIN FUNCTIONS screen and then select Reagents Log The following screen appears where it is possible to export the List of Reagent Movements press Export to copy it After exp...

Page 65: ...ollowing screen appears where it is possible to export the Events Log press Export to USB to copy it After exporting plug the USB in a PC a folder named DecalMATE_S N is present containing the file Ev...

Page 66: ...a year a technical preventive maintenance to maintain the high performance and prevent any malfunctioning Contact annually your local distributor or Milestone offices directly customersupport mileston...

Page 67: ...dual reagent in the drawer In this case empty it take it out and pour the residual reagent in a dedicated container 7 4 Twice a week Clean the area below the tanks and the related pipes 7 5 Weekly Cle...

Page 68: ...sette for BoneSTATION and DecalMATE CODE 67850 Rack 16 megacassette for BoneSTATION and DecalMATE CODE 67855 Rack 6 Supermega cassette for BoneSTATION and DecalMATE CODE 51413 5L tank MoL DECALCIFIER...

Page 69: ...ying solution for too long time 2 Section not well fixed before placing in decalcifying solution 1 Ensure that tissues containing calcium are placed in decalcifying solution 2 Surface decalcification...

Page 70: ...dvise to checking the specimens every 30 60 minutes In case faster turnaround time is needed it is possible to test at 50 C but in this case we strongly advise checking the specimens every 30 minutes...

Page 71: ...Check that the hose connector is clean and properly inserted Then press Clear to repeat the loading procedure 7 Call your customer service if the issue can t be fixed and press Help Desk to manage sp...

Page 72: ...over and verify if the reagent is in the cavity Then press Clear to reset the alarm 3 Call your customer service to fix the issue and press Help Desk to manage specimens while the Alarm is occurring 3...

Page 73: ...t the correct step to start from in the following table If wetness level appears NO HYDRATION A Follow the recovery laboratory internal protocols for air dried samples If not available follow Mileston...

Page 74: ...ater for 30 minutes 2 Place in formol glycerol solution until tissues become wet Tissues may remain in formol glycerol for up to 8 hours without adverse results 3 Reprocess on Fixation Decalcification...

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Page 80: ...marketing milestonemedsrl com www milestonemed com Via Fatebenefratelli 1 5 24010 Sorisole BG Italy Tel 39 035 4128264 Fax 39 035 575498 Milestone Srl...

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