Milestone DecalMATE, Operator'S Manual

Page: 70 / 80

DecalMATE MM112-003 - Operator Manual
70
A.2. BONE DECALCIFICATION GUIDELINE
1. If your decalcifying reagent is a PURE CHELATING (EDTA) SOLUTION WITH NO BUFFER (e.g. MoL-
DECALCIFIER):
For the first run the decalcification time setting can be roughly calculated by dividing your routine
decalcification time by 2.5 at 37°C, by 5 at 50°C,.
i.e.: routine room temperature decalcification time 25 hours; DecalMATE set time: 10 hours at 37°C.
i.e.: routine room temperature decalcification time 25 hours; DecalMATE set time: 5 hours at 50°C.
2. If your decalcifying reagent is a WEAK ACID LIKE FORMIC ACID 10-12%:
For the first run the decalcification time setting can be roughly calculated by dividing your routine
decalcification time by 5 at 37°C, by 10 at 50°C.
i.e.: routine room temperature decalcification time 8 hours; DecalMATE set time: 1 hour 40 minutes at 37°C.
We strongly advise to checking the specimens every 30-60 minutes.
In case faster turnaround time is needed, it is possible to test at 50°C but in this case we strongly advise
checking the specimens every 30 minutes.
i.e.: routine room temperature decalcification time 8 hours; DecalMATE set time: 50 minutes at 50°C.
3. If your decalcifying reagent is a MINERAL ACID LIKE HYDROCHLORIC ACID 10%, NITRIC ACID 7% or
BUFFERED EDTA (10% HCl):
For the first run the decalcification time setting can be roughly calculated by dividing your routine
decalcification time with HYDROCHLORIC/NITRIC ACID by 15 at 37°C.
i.e.: routine room temperature decalcification time 3 days; DecalMATE set time: about 5 hours.
We strongly advice to check the specimens every 15-20 minutes.
The following are guidelines for use with each decalcifying solution in use
in the laboratory
For mineral acids it is not possible to exceed the temperature above 37°C.
In the case of protocols running for days, solution exchange must be planned daily or
each second day.
Checking the bone at least every day is recommended.