Setup Procedures for Cell Salvage
P/N 53063-50, Manual Revision: B
4-6
Figure 4-7: Hang the anticoagulant solution from the IV pole
!
Connect the Aspiration and Anticoagulation set to the reservoir. Attach
suction source to reservoir and
set suction at a minimal acceptable flow
rate (under 200 mmHg).
Close the clamp on the anticoagulant IV drip
line.
Warning: When using a vacuum source, vacuum greater than 200 mmHg may
cause hemolysis.
!
Hang the anticoagulant solution from the IV pole and then aseptically
insert the spiked end of the drip chamber into the anticoagulant solution.
Be sure the bag is properly labeled as anticoagulant.
Note: The most common anticoagulant solution is 30,000 units of heparin in 1L
of normal saline. This should be delivered at a 1:7 ratio for anticoagulant to blood
entering the reservoir by adjusting the roller clamp on the anticoagulant line to
approximately 1 drop per second.
Note: Citrate solution can also be used as an anticoagulant solution. A general
guide for Citrate solution delivery is a ratio between 1:5 and 1:10 anticoagulant
to blood. the rate for both anticoagulants should be set to give approximately 15
ml of anticoagulant for each 100 ml of blood collected. A drip rate of one drop
per second is generally adequate.
!
Reopen the clamp on the anticoagulant drip line to allow full flow of
anticoagulant solution. Allow approximately 100 ml of anticoagulant to
flow into the collection reservoir to adequately prime the filter/defoamer
media, and adjust to desired drip rate. (Open clamp on the reservoir drain
only after the entire CS5+ disposable set has been installed and you are
ready to process blood in the CS5+.)
