P/N 53063-50, Manual Revision: B
Sequestering on the Cell Saver 5+ System
7-3
O
VERVIEW
OF
THE
SEQUESTERING
PROTOCOL
The Sequestering Protocol is usually performed before the Cell Saver Protocol.
After the power on and self-tests complete, the system is set up with the standard
bowl processing set. The Sequestering Kit (List No. 244) is attached to the
standard bowl processing set as described in this chapter for the sequestration of
platelet-rich plasma (PRP) and/or platelet-poor-plasma (PPP).
Note: The sequestering protocol is not available for use with the 70 mL bowl.
Two methods exist for performing sequestering: Manual Sequester Mode and
Assisted Sequester Mode. For both methods, blood is collected into blood bags
containing anticoagulant. The blood is separated into PRP, PPP, and red blood
cells (RBCs). Typical processing time is 25 to 40 minutes.
The sequestering process is identical for both methods. The Assisted Sequester
Mode provides preprogrammed processing parameters for optimal performance
along with prompts to assist the operator with steps of the process. The Manual
Sequester Mode allows the operator to select desired processing parameters and
provides no operator prompts.
Loading the
Disposable Set
After the system has been turned on and the machine self-test has been
completed, the operator will be prompted to load the disposable. Install the List
No. 244 Sequestering Kit as described below.
Before any blood can be processed, the Cell Saver disposable must be loaded
correctly. Follow the directions for loading the standard processing kit as
described in Chapter 4.
Step 1:
Open the centrifuge cover.
Step 2:
Open the LN 244 Disposable Kit. Locate the poly bag containing
the removal tool and open by tearing the perforated strip.
Step 3:
Use the removal tool to pry off the effluent line from the outlet port
of the Cell Saver 5+ bowl.
Note: Save the removal tool for later use.
Step 4:
Remove the cap from the bowl connector of the LN 244 sequester-
ing harness.
Step 5:
Place the bowl connector cap on the waste bag effluent line.
Step 6:
Install the bowl connector of the LN 244 sequestering harness to
the outlet port of the centrifuge bowl.
