P/N 53063-50, Manual Revision: B
Equipment and Disposable Description
2-17
Warning: Prior to pumping blood through the CS5+ harness and bowl, the blood
must be anticoagulated, either systemically or regionally. Non-anticoagulated
blood or blood components introduced into the bowl/ harness assembly will
clot. Such clotting renders the final blood product inappropriate for reinfusion.
Anticoagulant recommendations presented in this manual are intended as
guidelines only (refer to Chapter 3 for recommendations). For hypercoagulable
patients, the operator may find it necessary to increase the anticoagulant dosage
to prevent clotting.
When the bowl is used for cell washing, the volume of red cells gives a cell/
supernatant interface as shown in Figure 2-12. Filling the bowl to the appropriate
level as shown will give an hematocrit of approximately 50% for the washed
cells.
Figure 2-12: Red cell / interface
The hematocrit may be increased by filling the bowl until the red cell interface is
closer to the center of the bowl, although manually regulating the interface is not
advised. Conversely, a lower hematocrit will result if filling is terminated before
the red cell interface reaches the level shown in Figure 2-12. Under Automatic
control, the interface is controlled by the bowl optics sensor to produce
consistently good results.
In general, filling the bowl to a lower hematocrit will necessitate a higher volume
of wash solution (two times the standard wash volume) to dilute the supernatant
and achieve an adequate wash out.
Emptying red cells from the bowl is accomplished by bringing the centrifuge to a
complete stop, and then reversing the flow in the pump tubing. The centrifuge
may briefly rotate in both directions to facilitate the removal of the red cells from
the bowl.
Red Cell/Supernatant
Interface
