Ch. 6 - Calibration and Measurement
Calibration
User's Guide to the SPOT Cooled Color Digital Camera, 6/9/98
104
Introduction
The SPOT software provides you with the means to calibrate the objectives on your
microscope. You can then use the calibrated objectives to perform different
measurements on an image. Both the Calibration and the Measurement functions are
performed through the Show Measurements option on the View menu.
Calibration
Before using the SPOT software to perform measurements on images, you should
calibrate each of the microscope's objectives using a stage micrometer. To calibrate an
objective, follow these steps:
1.
Place the stage micrometer in the objective's field of view.
2.
Focus on it.
3.
Capture an image of the stage micrometer.
!
Note: For details on capturing an image, refer to Chapter 4, Taking a Picture.
4.
From the View menu, select the Show Measurements option (or press
[Ctrl M]
). The
Measurement window appears, as shown below:
5.
The Calibration Setup box should be blank. To calibrate the objective that you are
using, click on the Add button.
−
To modify an existing Calibration Setup, click on the Modify button.
−
To delete an existing Calibration Setup, click on the Delete button.
