COULTER STKS, Operator'S Manual, Page: 81 / 238

CYCLING SAMPLES
14. Incubate for a minimum of 5 minutes at
room temperature of 16 - 30°C (61 - 86°F).
Up to 60 minutes is OK. You can incubate
samples for up to 60 minutes to allow for
batching.
Repeat steps 10 through 14 for a batch of
samples.
15. Gently mix "A" tube. Aspirate 2 µL of the
sample/stain mixture from "A" tube.
7170037A
2 µ L
A
IMPORTANT
To prevent drying of this small amount of
sample, when you finish step 16, go immediately
to step 18. Read step 17 and the IMPORTANT
that goes with it now.
16. Dispense 2 µL of the sample/stain mixture
directly into the bottom of the "B" tube. Do
not let it run down side of tube.
A
B
7170047A
IMPORTANT
To keep bubbles or foam from forming, let
Reagent B run down the side of the tube.
Dispense Reagent B quickly enough to mix the
reagent and blood mixture. DO NOT MIX
FURTHER.
2 mL
B
7170048A
PN 4237188A (December 1993)
71