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CYCLING SAMPLES

Secondary Mode: Reticulocytes

IMPORTANT

Perform the reticulocyte dilution method according to the instructions in the package insert and
below. Modifications to the procedure, or failure to follow these instructions, may lead to misleading
or erroneous results.

Prepare the sample using the COULTER ReticPrep reagent kit.

1.

ANALYZER

Set the MODE OF OPERATION to RETICS
so that

CBC

↓

DIFF

↓

RETICS

↑

.

MAIN

SYSTEM CONFIGURATION

MODE OF OPERATION
Press the key opposite the test
configuration to set to RETICS.

MAIN MENU

or

RETURN

*SYSTEM RUN*

STATUS:
ALERT:

READY (RETICS MUST BE DILUTED)

NUMBER OF ASPIRATIONS/TUBE   01

(MESSAGE AREA)

MAIN

03/14/93
10:45:37

BD

CBC

DIFF

RETICS

7188083A

PN 4237188A (December 1993)

67

Summary of Contents for STKS

Page 1: ...COULTER STKS Analyzer with Reticulocyte Analysis Operator s Guide COULTER STKS PN 4237188B March 1995 COULTER CORPORATION Miami Florida 33196...

Page 2: ...used in a manner other than specified Operate the instrument as instructed in the Product Manuals You introduced software that is not authorized by Coulter into your computer Only operate your system...

Page 3: ...STARTUP RESULTS 16 DAILY QUALITY CONTROL 19 LATRON 19 RETIC C Cell Control 26 5C Cell Control for CBC Diff 33 Preliminary Steps 34 5C Cell Control for CBC DIFF Test in the Primary Mode 35 5C Cell Con...

Page 4: ...ES 56 Primary Mode CBC CBC DIFF 56 Secondary Mode CBC CBC DIFF 61 Secondary Mode Reticulocytes 67 Hgb Cuvette Drain 72 DATA ANALYSIS 75 CBC HISTOGRAMS 75 RBC Distribution Curve 75 Plt Distribution Cur...

Page 5: ...AVERAGE CALIBRATION FACTORS 93 TRANSMIT CALIBRATION FACTORS 94 HGB READINGS 94 START UP TESTS 95 HGB LAMP ADJUST AND TRANSMIT TO DMS 96 HGB LAMP ADJUST 96 TRANSMIT TO DMS 96 SET DATE FORMAT 97 SET TIM...

Page 6: ...THE WORKLIST 122 REQUIRED POSITIVE IDENTIFIERS 123 SETTING UP POSITIVE AND OPTIONAL IDENTIFIERS 124 SETTING UP LOCATION AND PHYSICIAN LISTS 125 AUTOMATIC SEQUENCING ON THE WORKLIST 126 TO ADD AN ENTR...

Page 7: ...llation 151 Preliminary Report 152 DATA BASE BATCH PROCESSING 152 ARCHIVING FROM DATA BASE 154 To Review Archived Files 155 DELETE DATA BASE 157 CONTROLS 159 EXPECTED RANGES 159 SET AUTO STOP OPTION 1...

Page 8: ...E 186 APPENDIX A AUTO REPORTER 3 187 CONFIGURATION 188 MESSAGES ON THE AUTO REPORTER 3 DISPLAY 189 MESSAGES THAT PRINT ON THE AUTO REPORTER 3 TICKET 190 CRC ERROR 190 D disabled checksum 191 E edited...

Page 9: ...OGARD Closure 208 BECTON DICKINSON Plastic Tubes with HEMOGARD Closure 208 COULTER 209 LABO EXPRESS SERVICE LES 209 LDM 209 SHERWOOD MEDICAL 209 TERUMO VENOJECT Tubes 209 TERUMO VENOJECT II Foil Top 2...

Page 10: ...S with Retic Analysis Workflow Scenarios 49 5 CBC DIFF Parameter Codes 79 6 Retic Codes and Messages 82 7 Summary of Flagging Messages 85 8 Definitive Flagging Limits 85 9 Transmit Messages 87 10 Stat...

Page 11: ...um isolator Use this precision REGULATOR to adjust the vacuum in the vacuum isolator The LIGHT BAR lights the apertures Use this ACTIVATOR to start a cycle in the secondary mode Use the DILUTER KEYPAD...

Page 12: ...AIN MENU 1 2 3 7 8 4 RETURN 1 DILUTER SWITCH PANEL ALARM IS EN DISABLED 2 LONG ALARM IS EN DISABLED 3 CONTINUOUS ALARM IS EN DISABLED 7 MAIN MENU 8 RETURN 1 RAMP TEST 2 PRECISION TEST 3 BACKGROUND TES...

Page 13: ...such as Startup and cannot receive another command PRIME The system is priming itself before collecting data READ HGB The system is measuring Hgb concentration photometrically The Analyzer is computi...

Page 14: ...urrent burn cycle Rotates and rinses mixing chamber When pneumatics are ON Drains and rinses baths Backwashes both modes Applies 6 vacuum to apertures to remove bubbles from lines Rotates and rinses m...

Page 15: ...DILUTER KEYPAD Insert 11 x 17 pages here pages 5 and 6 Page 6 has a menu summary but no page number on it PN 4237188A December 1993 5...

Page 16: ...CONTROLS AND INDICATORS 6 PN 4237188A December 1993...

Page 17: ...2 21 4 9 0 ID 1 ID 2 Data 01 09 89 Time 10 10 09 COMPUTER POWER BUTTON ALL FUNCTION KEYS ARE IN THIS ROW COMPUTER ALL FUNCTION KEYS ARE IN THIS ROW MONITOR POWER BUTTON before a key or procedure tells...

Page 18: ...the cycle a momentary vacuum drop below 17 Hg does not constitute a system failure The PNEU TEMP Pneumatic temperature lamp lights when the pneumatic assembly is too hot the pneumatics subsystem turns...

Page 19: ...ouping asks you to press the POWER ON key of the Diluter keypad For example If the power is off press DILUTER POWER ON and wait 30 minutes for warmup ANALYZER DILUTER POWER ON DMS CHECK REAGENTS Check...

Page 20: ...Error Log F5 Other F9 Main menu F10 Previous menu 10 14 93 10 15 OPR DMS PR TK HC DB XB WL HWL QC 59 5 Press to return to Main Menu 6 Select Start Up DMS AT THE ANALYZER CRT ANALYZER 1 Check for the R...

Page 21: ...N MENU RETURN DILUTER CONFIGURATION NUMBER OF BARCODE DIGITS 2 OF 5 11 BLOOD DETECTOR IS ENABLED MODE OF OPERATION CBC DIFF 7188038A MESSAGE AREA DILUTER CONFIGURATION LEVEL SENSE Check settings RETUR...

Page 22: ...etween 105 V and 125 V or 210 V 250 V CONTROL VOLTAGE lamp is lit All MONITOR LAMPS are unlit WARNING The contents of the vacuum trap bottle may include residual biological material and must be handle...

Page 23: ...A COULTER CUSTOMER OPERATIONS 1 800 526 7694 7188032A AT THE DILUTER 1 Verify that the top bar on the electronic manometer is in the green range If not move the vacuum adjust regulator to the right to...

Page 24: ...ut toward you and lay it aside DILUTER DRAIN Check that both aperture baths drain completely DILUTER RINSE both baths fill with liquid waste chamber drains 7188014A LIGHT BAR APERTURE BATHS 3 Push the...

Page 25: ...OUNT appears on the Analyzer CRT check that There are no bubbles in the 3 lines from each of the aperture baths which run through VL13 and VL16 to the vacuum isolator Diluent drips steadily from these...

Page 26: ...k 11 22 93 HCT 83 83 HCT 47 17 MCV 165 5 MCV 73 8 DIFF TEST 08 20 93 MCH 28 06 MCH 22 25 MCHC 16 94 MCHC 30 12 Diff Pressure 0 37 RDW 47 67 RDW 14 23 Sheath Pressure 6 38 PLT 276 4 PLT 798 7 Laser Vol...

Page 27: ...D TEST If results are still not acceptable call Coulter Customer Operations Return to the System Run display 4 Check the ramp and precision tests If any test value exceeds the reference value by 1 or...

Page 28: ...ach or cleaning agent in the baths d Repeat the ramp or precision test Press keys next to ANALYZER ANALYZER FUNCTIONS START UP TESTS RAMP TEST or PRECISION TEST e If you see a flag this time 1 2 3 4 5...

Page 29: ...imately 1 5 mL of LATRON Functions for using LATRON Diff only F55 Retic only F56 Diff Retic F57 Coulter recommends using F57 for monitoring Diff and Retics with the LATRON The following procedure is f...

Page 30: ...n the lower front cover 7188036A 4 Wipe the aspirator tip thoroughly using distilled water and a lint free tissue 7188060A 5 Lift the lower front cover back into place 6 DILUTER PRIME APERT 20 PN 4237...

Page 31: ...HC DB XB WL HWL QC 48 The selected CONTROL RUN file appears The box in the lower right corner of the screen tells you which mode you are in and which function to use for this mode In a Diff Retic Late...

Page 32: ...O P 7 8 9 4 5 6 1 2 3 CE 0 F ID ENTER PRIME APERT 7 1 8 8 0 0 8 A PRESS MANUAL 12 a Immerse the aspirator tip in the LATRON primer vial b Press and release the activator Message is LATRON DIFF RET DIL...

Page 33: ...CALL COULTER CUSTOMER OPERATIONS IN THE USA 1 800 526 7694 COUNT 500 CONTINUE YES NO YES COUNT 500 YES 1 2 STEP 12 3 CHECK RESULTS DO THIS UP TO 3 TIMES NO DILUTER ENTER DATE 03 16 93 TIME 08 32 15 CO...

Page 34: ...F ID ENTER PRIME APERT 7 1 8 8 0 0 8 A PRESS MANUAL 16 Gently mix the control Invert the vial five to eight times Avoid foaming 7188010A 17 a Immerse the aspirator tip in the LATRON control vial b Pre...

Page 35: ...tter S 90 9 90 0 0 9 5 0 CV RESULTS EXPECTED CV Volume V 3 6 7 0 Diff Retic Conductivity C 5 1 10 0 REMEMBER TO RUN Scatter S 3 2 9 0 IN FUNCTION 57 F1 Help F2 File F3 Primer F4 Prt F5 Histo F6 Graph...

Page 36: ...Use F44 CLEAR FLOW CELL 1 2 If you see the flow cell clogged message on the CRT use F45 CLEAR FLOW CELL 2 up to 3 times 3 If the flow cell remains clogged aspirate LATRON primer using F55 up to 3 time...

Page 37: ...urn the AutoStop option on see AutoStop in the CONTROLS chapter 3 At the Main Menu press DMS Other Prime to turn Prime ON Sample Analysis Controls Start Up Special Functions DMS 2A Other LTER F3 AR3 B...

Page 38: ...C DB XB WL HWL QC 54 7 Select DMS Controls Control Run Press File Move the cursor to the file you want Press RETIC CONTROL RUN FILE 18 LOT 539900 OPR hs SHIFT 0 LEVEL Level III EXP DATE 11 26 93 RUN 2...

Page 39: ...START UP SHUT DOWN ALARM RESET P R E M IX S T O P 7 8 9 4 5 6 1 2 3 CE 0 F ID ENTER PRIME APERT 7188082A READY NO BLOOD 9 Make sure the repipetter is fitted securely to the Reagent B bottle 7170005A 1...

Page 40: ...reagent at an angle changes the dilution of the preparation 12 Holding the dropper bottle vertically place 4 drops of Reagent A into the A tube 7170002A A A 13 Dispense 50 L of well mixed Retic C cel...

Page 41: ...p 18 and the IMPORTANT that goes with it now 17 Dispense 2 L of the sample stain mixture directly into the bottom of the B tube Do not let it run down side of tube 7170007A 2 L B A IMPORTANT To keep b...

Page 42: ...field displays WIPE d Wipe the tip with a lint free tissue 7188037A 20 Check the results on the RETIC CONTROL RUN screen RETIC CONTROL RUN FILE 18 LOT 539900 OPR hs SHIFT 0 LEVEL Level III EXP DATE 11...

Page 43: ...l is read or when you enter the lot number in the Secondary mode properly the DMS automatically Identifies the control by level lot expiration date and shift Checks control expiration date against cur...

Page 44: ...own means and expected ranges for each parameter See Controls chapter 2 If you want to turn the AutoStop option on see the Controls chapter 3 ANALYZER MAIN SYSTEM CONFIGURATION MODE OF OPERATION Set...

Page 45: ...al Functions DMS 2A Other LTER F3 AR3 Barcode On STKS F5 AutoPrint NONE F6 AutoTransmit Off F7 Prime mode On Operator ID OPR ooooo ooooo oooooooooooo oooooooooooo ooooooooo CBC Diff Retic 932231 COPYR...

Page 46: ...high read rate keep the bar code label free of blood scratches and powder from gloves 6 Prepare 5C cell controls according to directions in the package insert 7 Put the control tubes Normal Abnormal...

Page 47: ...0 0 6 NE 53 8 53 7 0 1 5 0 HCT 49 2 49 1 0 1 2 7 NE 4 9 5 0 0 1 2 0 MCV 87 3 87 6 0 3 3 0 LY 29 4 29 4 0 0 5 0 MCH 29 5 29 6 0 1 1 2 LY 2 7 2 7 0 0 1 6 MCHC 33 8 33 8 0 0 1 7 RDW 14 1 13 9 0 2 1 5 MO...

Page 48: ...t determine its own means and expected ranges for each parameter and set up separate files with separate lot numbers for Secondary mode use See Control File Set Up in the Controls chapter for entering...

Page 49: ...f F7 Prime mode Off Operator ID OPR ooooo ooooo oooooooooooo oooooooooooo ooooooooo CBC Diff Retic 932231 COPYRIGHT 1989 1993 COULTER CORPORATION ALL RIGHTS RESERVED F1 Help F3 Error Log F5 Other F9 M...

Page 50: ...lease the activator c Remove the control when you hear the beep and the Analyzer CRT status field displays WIPE d Wipe the tip with a lint free tissue 7188057A 8 View the results on the CBC DIFF CONTR...

Page 51: ...6 2 7 NE 5 3 5 1 0 2 2 0 MCV 87 7 86 9 0 8 3 0 LY 30 1 29 3 0 8 5 0 MCH 29 4 29 8 0 4 1 2 LY 3 0 2 7 0 3 1 6 MCHC 33 5 34 2 0 7 1 7 RDW 13 7 13 3 0 4 1 5 MO 7 9 8 6 0 7 3 0 MO 0 8 0 8 0 0 0 8 PLT 236...

Page 52: ...ADY NUMBER OF ASPIRATIONS TUBE 01 MAIN 03 14 93 10 45 37 BD CBC DIFF RETICS 7188089A 2 From the Main Menu press Other DMS Prime to turn Prime on 3 Press DILUTER PRIME APERT Then cycle a normal whole b...

Page 53: ...ESULTS ASSAY DIFF LIMITS WBC 19 2 0 9 RBC 5 23 0 14 HGB 16 6 0 4 HCT 47 8 2 5 MCV 91 4 3 0 MCH 31 7 1 5 MCHC 34 7 2 2 RDW 13 8 2 0 PLT 389 40 MPV 10 4 2 0 REMEMBER TO DISABLE THE DIFF F1 Help F2 File...

Page 54: ...RBC 5 20 5 19 0 1 0 14 HGB 16 5 16 5 0 0 0 4 HCT 47 1 47 0 0 1 2 5 MCV 90 5 90 6 0 4 3 0 MCH 31 7 31 8 0 1 1 5 MCHC 35 0 35 1 0 1 2 2 RDW 13 6 13 3 0 3 2 0 PLT 397 385 12 40 MPV 10 9 10 5 0 4 2 0 REME...

Page 55: ...BC CONTROL FILE REVIEW in the CONTROLS chapter 13 If the control is outside limits the system displays an error message stops if AutoStop is on status line shows QC turns QC red flags the result with...

Page 56: ...the Diluter keypad Follow the directions in these procedures and enter the ID correctly Rerun Control file set up incorrectly Verify that the assigned values and ranges match those on the control s pa...

Page 57: ...r CBC with WBC differential Use for samples with very low hemoglobin results when Primary mode run produces a partial aspiration Blood detectors do not recognize these samples as blood Use for Reticul...

Page 58: ...ic A test or combination of tests for a single patient specimen is a profile Profiles are for collating purposes Each profile has a number Default profiles are Profile Number Test s 1 CBC Diff 2 CBC 3...

Page 59: ...LE IN WORKLIST FOR EXAMPLE 1 4 2 YES Table 4 STKS with Retic Analysis Workflow Scenarios Scenario What to do Benefits 1 No DMS Collation No Preassignment Retics CBC Diff and CBC are run and reported i...

Page 60: ...ts RET and RET a Access Worklist Add b Select CBC Diff Retic profile c Assign patient ID for each test add demographics as desired for next sample d Analyze samples as in Scenario 1 Useful if lab is c...

Page 61: ...tion benefits 2 Demographics on lab report 3 Maintains walkaway CBC Diff workflow 5 Manual Collation by Editing Retic to CBC or CBC Diff Report Manually add Retic result to CBC or CBC Diff in DataBase...

Page 62: ...whole blood is aspirated 50 L whole blood for Retic preparation IMPORTANT Be sure there is a bubble or space in each sample tube to facilitate mixing LEAVE SPACE 7188046A STORING SPECIMENS 1 Venipunct...

Page 63: ...CLS publication H18 A PRIMING WHEN TO PRIME Prime the system by pressing on the Diluter and cycling a PRIME APERT normal whole blood sample for CBC or CBC DIFF or prepared reticulocyte sample for RETI...

Page 64: ...same number of digits This number is set on the Analyzer CRT in SYSTEM CONFIGURATION You cannot mix the number of digits stored in the DMS data base If you do this message appears on the DMS ONLY XX N...

Page 65: ...and corners 4 The bars on the label must be parallel to the stopper When your system is modified for tubes with HEMOGARD Closure labels on regular tubes must be placed 0 5 in down from the cap Positio...

Page 66: ...e additional thickness 7188049A CYCLING SAMPLES WARNING If a problem occurs while the system is cycling press and wait for the system to DILUTER STOP stop before you do anything to correct the problem...

Page 67: ...AIN SYSTEM CONFIGURATION MODE OF OPERATION Press the key as needed to set test mode MAIN MENU SYSTEM RUN SYSTEM RUN STATUS ALERT READY NUMBER OF ASPIRATIONS TUBE 01 MESSAGE AREA MAIN 03 14 93 10 45 37...

Page 68: ...keypad DILUTER 1 ENTER POWER ON D R A IN R IN S E CLEAR APERT START CONT POWER OFF START UP SHUT DOWN ALARM RESET P R E M IX S T O P 7 8 9 4 5 6 1 2 3 CE 0 F ID ENTER PRIME APERT 7 1 8 8 0 5 1 A VALUE...

Page 69: ...n the Worklist do so now See Worklist chapter Worklist Current line 1 Entries 7 7 Tagged 0 CBC DIFF Cass pos ID 1 ID 2 Sequence Tests Status 001209 0825001 Jones Sam CDR 001210 0825002 Miller Betty CD...

Page 70: ...cassette has a bar code label that identifies the cassette and each position in it This gives you sample traceability but it does not positively identify the sample Slide the tubes in firmly Be sure t...

Page 71: ...he Secondary mode for manual aspiration Bar code labels are not read in this mode you must enter an ID number via the Diluter keypad before cycling the sample You can use only number characters to ide...

Page 72: ...OF OPERATION Press the key as needed to set test mode SYSTEM RUN STATUS ALERT READY NUMBER OF ASPIRATIONS TUBE 01 MESSAGE AREA MAIN 03 14 93 10 45 37 BD CBC DIFF RETICS 7188050A MAIN MENU SYSTEM RUN 2...

Page 73: ...the STATUS message on the Analyzer CRT changes from INTRO SAMPLE to WIPE and there is a beep WARNING The aspirator tip retracts when the PRIME message appears in the STATUS field on the Analyzer CRT b...

Page 74: ...cycling is completed DMS Other Prime to turn Prime off i Check that all the F5 Options are correct for your laboratory 3 To enter the sample ID number a DILUTER ID WANT TO ENTER CASS POS NO YES NO GO...

Page 75: ...ASSETTE POSITION X X X X X X 7188061A c Enter a 3 11 digit ID 1 for the patient sample ALL DIGITS FOR ID 1 ENTER X 7188062A X X d ANALYZER Check the numbers on the Analyzer CRT to be sure they are cor...

Page 76: ...CRT changes from INTRO SAMPLE to WIPE and there is a beep WARNING Be sure to remove the sample tube and be clear of the area around the aspirator tip before the STATUS field displays the PRIME messag...

Page 77: ...r erroneous results Prepare the sample using the COULTER ReticPrep reagent kit 1 ANALYZER Set the MODE OF OPERATION to RETICS so that CBC DIFF RETICS MAIN SYSTEM CONFIGURATION MODE OF OPERATION Press...

Page 78: ...E APERT 4 Cycle a prepared reticulocyte sample as a prime 5 Other DMS Prime to turn Prime off Sample Analysis Controls Start Up Special Functions DMS 2A Other LTER F3 AR3 Barcode On STKS F5 AutoPrint...

Page 79: ...ETTE POSITION X X X X X X 7188061A c Check the numbers on the Analyzer CRT to see if they are correct 8 Make sure the repipetter is fitted securely to the Reagent B bottle 7170005A 9 Prime Reagent B m...

Page 80: ...t at an angle changes the dilution of the preparation 11 Holding the dropper bottle vertically place 4 drops of Reagent A into the A tube A A 7170043A 12 Dispense 50 L of well mixed sample into the A...

Page 81: ...A IMPORTANT To prevent drying of this small amount of sample when you finish step 16 go immediately to step 18 Read step 17 and the IMPORTANT that goes with it now 16 Dispense 2 L of the sample stain...

Page 82: ...CRT changes from INTRO SAMPLE to WIPE and there is a beep c Remove the dilution when you hear the beep and the Analyzer CRT STATUS field displays WIPE d Wipe the tip with a lint free tissue 7188037A 1...

Page 83: ...is run and no sample has been run in the Primary mode The drain and rinse routine consists of these functions The Hgb cuvette drains into the waste chamber The RBC bath drains into the waste chamber T...

Page 84: ...SAMPLE ANALYSIS 74 PN 4237188A December 1993...

Page 85: ...The normal RBC curve ranges from 36 to 360 fL The display starts at 24 fL 5935 141 Plt Distribution Curve Normal Plt distribution yields two curves both using averaged data The smooth curve derives fr...

Page 86: ...DF 1 Scatterplot DF 1 Discriminant Function 1 is the scatterplot that automatically appears with CBC Diff sample data It is derived primarily from volume and light scatter The Y axis represents volum...

Page 87: ...s are the prominent populations The granulocytes include the neutrophils basophils and eosinophils DF 3 Scatterplot DF 3 displays the same data as DF 2 with the neutrophil and eosinophil populations g...

Page 88: ...primarily for troubleshooting by Service RETICULOCYTE SCATTERPLOTS DF 5 Scatterplot This is the initial display of Retic analysis It derives primarily from light scatter and volume The Y axis represe...

Page 89: ...uses more decimal places than it displays and rounds off to the result you see on the screen thus maximizing precision CBC DIFF Codes If an R flag occurs review results Table 5 CBC DIFF Parameter Code...

Page 90: ...103 MCV 1000 WBC 100 0 x 103 If WBC is overrange an R appears next to the RBC Hgb Hct MCV MCH MCHC RDW MPV Plt and Diff results Results for Diff are dots If RBC is overrange an R appears next to Hct M...

Page 91: ...V results PDW 20 mode not between 3 and 15 or non positive curve detected or Plt 20 000 or Total voteout of fitted curve or WBC is overrange RBC Parameters R next to RDW result Excessive asymmetry in...

Page 92: ...ated from edited or manually entered RBC or Retic EDITED DATA RET XXXXE Operator edited RET EDITED DATA RET XX XXE Operator edited RET EDITED DATA RET XX XX RET XXXXR RET 30 0 VERIFY RETIC RET XX XXR...

Page 93: ...BC Plt and Retic population status only If there is a voteout for WBC RBC or Plt no message appears for the respective parameter If CBC only is performed population messages refer to WBC RBC and Plt c...

Page 94: ...tes these messages according to an internal algorithm These messages appear in the Cell Classification window on the Run Samples screen toggles this window on and off Confirm any abnormality by micros...

Page 95: ...s Hypochromia Poikilocytosis Erythrocytosis Pancytopenia Plt Normal Plt Pop Abnormal Plt Pop Edited Data No message Platelet Clumps Giant Platelets Thrombocytopenia Thrombocytosis Small Platelets Larg...

Page 96: ...m 2 Macrocytosis 1 Hypochromia Low limit for MCH 2 Hypochromia A gradient range from 1 Hypochromia 3 Hypochromia A gradient range from 2 Hypochromia 1 Poikilocytosis High limit for RDW and Low limit f...

Page 97: ...anually from the Analyzer via TRANSMIT TO DMS Ramp Appears when a Ramp test is sent from the Analyzer Precision Appears when a Precision test is sent from the Analyzer S Appears when a Secondary mode...

Page 98: ...DATA ANALYSIS 88 PN 4237188A December 1993...

Page 99: ...f the power at night and the STKS is going to be idle for more than 48 hours 1 Press Let cleaning agent remain in the instrument for at least 30 minutes SHUT DOWN 2 Press to put diluent in the lines S...

Page 100: ...If 24 hours elapse with the power on the pneumatics off and the instrument in shut down the system automatically Turns on the pneumatics Displays the message AUTOPURGE on the Diluter keypad Purges the...

Page 101: ...in the message area The MAIN MENU also displays the Analyzer cycle counter This counter increments at the beginning of these cycles All primary All secondary except Latex functions F55 F56 F57 Backgr...

Page 102: ...187 1 003 1 009 HGB 1 320 MCV 0 961 PLT 1 106 1 006 1 032 MPV 1 148 MAIN MENU RETURN MAIN MENU RETURN MESSAGE AREA This function displays the average AVG aperture 2 AP2 and aperture 3 AP3 calibration...

Page 103: ...ST TRANSMIT TO DMS CALIBRATION MENU CALIBRATION FACTORS PARAMETER WBC RBC HGB MCV PLT MPV CAL FACT 1 017 1 222 1 333 0 879 1 049 1 104 CALIBRATION FACTORS RECEIVE AVERAGE CALIBRATION FACTORS TRANSMIT...

Page 104: ...bration factors or the average and individual aperture factors Transmit the average factors at the beginning of calibration Transmit all factors when calibration is complete and you are ready to recor...

Page 105: ...READINGS START UP TESTS HGB LAMP ADJUST TRANSMIT TO DMS TIME DATE MAIN MENU RAMP TEST PRECISION TEST BACKGROUND TEST MAIN MENU RETURN CYCLE COUNTER XXXXXXX Use this function to perform the ramp precis...

Page 106: ...not cleaning agent in the baths to check the Hgb lamp voltage In the message area the message appears PERFORMING HGB LAMP ADJUST When the adjustment is complete the message appears LAMP VOLTAGE ADJUS...

Page 107: ...NCTIONS SYSTEM CONFIGURATION TURN PNEUMATICS OFF MAIN MENU DATE FORMAT U S SET TIME AND DATE CYCLE COUNTER XXXXXXX Use this function to select the date format Press the key next to DATE FORMAT to togg...

Page 108: ...T TRANSMIT TO DMS TIME DATE START UP TESTS MAIN MENU DATE FORMAT SET TIME AND DATE SET TIME DATE MONTH DAY YEAR HOURS MINUTES SECONDS MAIN MENU RETURN Use this function to set the time and the date On...

Page 109: ...ABLED CLEANER LEVEL SENSE IS EN DISABLED LYSE LEVEL SENSE IS EN DISABLED PAK LEVEL SENSE IS EN DISABLED MAIN MENU RETURN DILUTER CONFIGURATION BLOOD DETECTOR IS EN DISABLED MODE OF OPERATION IS CBC CB...

Page 110: ...disable the alarms Press the key next to the option to change the setting NUMBER OF BAR CODE DIGITS Key Sequence 2 2 CYCLE COUNTER XXXXXXX 7182003A MAIN MENU ANALYZER FUNCTIONS SYSTEM CONFIGURATION DI...

Page 111: ...sable the blood detector Set the mode of operation to CBC or CBC DIFF or RETICS Press the key next to the option to change the setting The current status of these two options is indicated by an arrow...

Page 112: ...n by pressing the key next to this line and entering the number on the Diluter keypad Maximum number of aspirations is 12 STATUS messages describe the status of the cycle They are NOT READY READY INTR...

Page 113: ...the condition of all the apertures You can detect noise bubbles or a clogged flow cell When DIFFERENTIAL is enabled the screen shows nine pulse windows one for each of the three apertures in the RBC...

Page 114: ...ETURN 4 HGB LAMP ADJUST 5 TRANSMIT TO DMS 6 TIME DATE 7 MAIN MENU 8 RETURN MAIN MENU 1 2 3 7 8 4 RETURN 1 DILUTER SWITCH PANEL ALARM IS EN DISABLED 2 LONG ALARM IS EN DISABLED 3 CONTINUOUS ALARM IS EN...

Page 115: ...one position to the right Moves Menu highlight bar to next Menu item Moves cursor one position to the left Moves Menu highlight bar to previous Menu item Moves cursor to end of the field Moves cursor...

Page 116: ...aracter to left of the cursor Clears a data entry field Clears a data entry field and displays the original value in the field Clears from the cursor to the end of a data entry field Clears error line...

Page 117: ...HC DB XB WL HWL QC 48 When you select Other from the Main Menu you have these options turns the AUTO REPORTER 3 bar code reader On and Off sets the Graphic Printer to automatically print NONE do not p...

Page 118: ...e DMS and the Auto Reporter 3 are electronically connected HC Host Computer AutoTransmit on Sample Analysis and Control Samples AutoTransmit off Sample Analysis and Control Samples Not connected to ho...

Page 119: ...is function to set the maximum number of records allowed in the Data Base and Worklist Data Base choice is from 500 to 5000 To enter a number that is less than the current number of samples in the Dat...

Page 120: ...genta RET CTRL Yellow OTHERS CARRYOVER REPRODUCIBILITY STARTUP BACKGROUND PRIME SERVICE TESTS The Workload Recording operation logs data for up to 365 days or a count of 1166400 When it reaches either...

Page 121: ...TED If the date received is less recent than the last sample received this message appears on the Error line and is posted to the Error Log Previous date rcvd Function keys available Help Print Graph...

Page 122: ...yzer Problem solved Page 0 of 150 931941 COPYRIGHT 1989 1993 COULTER CORPORATION ALL RIGHTS RESERVED F1 Help F2 Stamp F3 Print_Pg F4 Print F5 Other F6 Del F7 Archive PgUp PgDown 10 27 93 10 00 OPR DMS...

Page 123: ...0 OPR DMS PR TK HC DB XB WL HWL QC 43 Unable to archive CONTROL file File1 wks When an error message occurs on the DMS it is recorded in the Error Log When you run a batch of samples you can use the E...

Page 124: ...DMS BASICS 114 PN 4237188A December 1993...

Page 125: ...Run Samples The DMS screen displays the sample results The display includes scatterplot histograms parameter data and flagging codes and messages as they apply This example shows the CBC and Diff res...

Page 126: ...off the window that displays the Suspect and Definitive flags When you turn it off it stays off for succeeding samples also However if the Suspect and Definitive flag options in Printer Definition are...

Page 127: ...he current batch and indicates whether the last completed X B batch is in or out DB Storage Turns Data Base Storage on and off AutoPrint Sets automatic printing of the Graphic Printer to OFF NONE prin...

Page 128: ...rime mode ON OFF When you set Prime mode to ON the X B DB and AutoTransmit automatically turn off Operator ID Use to enter a three character operator identification B W screen print Sets the Graphic P...

Page 129: ...DB Use to look at samples stored in the Data Base Menu Returns display directly to the Main Menu CD or CR Use to toggle between CBC DIFF results and CBC RETIC results on a collated sample PN 4237188A...

Page 130: ...RUN SAMPLES DISPLAY 120 PN 4237188A December 1993...

Page 131: ...combination of tests to be performed The Worklist keeps a separate list for each test CBC CBC DIFF RETIC You can use the Worklist to preassign from 100 to 1000 patients depending upon the size of the...

Page 132: ...to the Worklist for the next test WHEN YOU PREASSIGN THE WORKLIST When you preassign a sample you select a profile and enter sample identification for each test The Worklist is test type oriented When...

Page 133: ...from the Worklist for that test If other tests for that sample have not yet been run the sample entry remains on the Worklist of the pending test As tests assigned in a profile are matched and removed...

Page 134: ...1 Yes nalysis set up OPTIONAL IDENTIFIERS Profiles 1 2 3 4 5 6 7 8 9 ID 2 ID 2 CBC Sequence Sequence Diff STATUS Status Retic Date of birth Date of Birth Sex Sex Location Location ction d Physician Ph...

Page 135: ...mergency Maternity tion limits ICU cation list 1st floor east ysician list 2nd floor west splay formats Wing A lete database Wing B ear print spooler queue set communications board set DMS TION ALL RI...

Page 136: ...PR DMS PR TK HC DB XB WL HWL QC 65 To go to the Worklist Auto sequencing window press Seq directly from the Worklist or from the Sample preassignment window WL Add or from the Sample editing window WL...

Page 137: ...h Sex User field 3 Comments F1 Help F4 Print F5 Auto Seq F6 Clear field F10 Save Esc 06 10 93 12 01 OPR DMS PR TK HC DB XB WL HWL QC 28 1 Press Add The screen changes to the Sample Preassignment windo...

Page 138: ...C Cass pos ID 1 ID 2 Sequence Tests Status 001208 063037 R Rooney 100014 CDR Sample preassignment Profile 3 Profiles 1 2 3 4 5 6 7 8 9 Cass pos ID 1 Status CBC CBC Diff 000403 617 Diff Retic 000506 71...

Page 139: ...09 04 93 08 27 Location 2nd floor south User field 1 Physician User field 2 Date of Birth Sex User field 3 Comments F1 Help F2 Physician list F4 Print F5 Auto Seq F6 Clear field F10 Save Esc 09 04 93...

Page 140: ...000505 713 Link Panettiere 100015 CDR 000506 714 Susan Durham 100016 CDR F1 Help F2 Add F3 Edit F4 Print F5 Seq F6 Del F7 Tag F11 HWL F12 DbQ Next test 09 04 93 08 31 OPR DMS PR TK HC DB XB WL HWL QC...

Page 141: ...See the Special Procedures and Troubleshooting manual PN 4237187 for Collate Failure messages PART ASP The system detected a partial aspiration PARTIAL ASP is posted in the Worklist Status field if t...

Page 142: ...message is posted to the Error Log Example PART ASP aspiration error on sample Cass Pos ID 1 If a total of 10 non consecutive PART ASP NO MATCH and NO READ messages accumulates the Analyzer stops unle...

Page 143: ...nt The Ticket printout is not automatically printed Results are not automatically transmitted to the host computer if the PART ASP NO READ option is set to NO However if the PART ASP NO READ option is...

Page 144: ...he third message occurs but the fourth sample has already been scanned and is in process ID MISMATCH The Positive Identifier is Cass Pos only and the system reads a tube number that does not match the...

Page 145: ...opriate error message is posted to the Error Log If any of these combinations of error messages occur both messages are posted to the Error Log Partial Aspiration and No Match Partial Aspiration and I...

Page 146: ...F3 Transfer F4 Print F6 Delete F7 Tag F8 Tag All F12 WL 10 14 93 09 49 OPR DMS PR TK HC DB XB WL HWL QC 21 If your system is connected to a host computer you can transfer sample identification data fr...

Page 147: ...ode Label XB COULTER STKS CBC Diff Retic 931483 COPYRIGHT 1989 1993 COULTER CORPORATION ALL RIGHTS RESERVED F1 Help F3 Error Log F5 Other F9 Main menu F10 Previous menu 06 02 93 08 31 OPR DMS PR TK HC...

Page 148: ...other samples change the Sort criteria If the last sorting process resulted in no entries displayed here then when you access this option the sort window appears The top of the screen shows you from...

Page 149: ...rwritten when wraparound occurs Place the cursor on the sample to be saved and press this key If you press the key again or if the sample is already saved it unsaves You can save up to 150 samples Goe...

Page 150: ...n screen Transmit Transmit highlighted sample to a host computer To transmit more than one sample use Batch mode WL Go to the Worklist DB When an entry on this screen is highlighted and you press the...

Page 151: ...93 S 2649 05 05 93 12 53 07 OPR TIME S 2650 05 05 93 12 55 00 OPR From To S 1538 05 05 93 12 56 45 OPR Cass pos S 1538 05 05 93 12 58 52 OPR From To S 4290 05 05 93 12 59 44 OPR ID 1 S 430 05 05 93 1...

Page 152: ...onologically by date and time 8 Blank sort criteria fields for DATE TIME Cass pos ID 1 ID 2 and STATUS FLAGS mean that all data for that criterion is selected 9 When B Both is selected for the STATUS...

Page 153: ...a have been set The samples you selected to be sorted are listed on the screen To view the graphic results of a sorted sample place the cursor on that line and press To view tabular results press Main...

Page 154: ...8 8 9 L 31 7 L 85 0 28 2 L 272 S 000 11 16 S 300 11 18 002201 11 24 002201 11 26 F2 Save F3 Edit F4 Print F5 Batch F6 Sort F7 Tag F8 Transmit F11 WL F12 DB Next 09 04 93 08 43 OPR DMS PR TK HC DB XB W...

Page 155: ...ME Status Retic S 202 09 01 93 11 15 45 CBC Diff 002202 101 09 01 93 11 06 41 Normal WBC Pop Abnormal RBC Pop Normal PLT Pop WBC 5 8 10 3 uL RBC 3 73 L 10 6 uL PLT 272 10 3 uL HGB 8 9 L g dL MPV 9 3 f...

Page 156: ...9 01 93 10 52 00 Abnormal WBC Pop Abnormal RBC Pop Normal PLT Pop WBC 5 7 10 3 uL RBC 3 69 L 10 6 uL PLT 276 10 3 uL HGB 8 9 L g dL MPV 9 5 fL NE 62 8 3 6 HCT 31 2 L LY 24 5 1 4 MCV 84 5 fL MO 10 4 H...

Page 157: ...lysis Data Base Query or Edit Reorder patient EDIT DB SAMPLE Cass pos ID 1 DATE TIME Status Retic S 200 09 01 93 11 14 46 CBC Diff 002201 100 09 01 93 10 58 51 Reorder patient Profile 2 Profiles 1 2 3...

Page 158: ...F6 Clear field F10 Save Esc 09 04 93 08 51 OPR DMS PR TK HC DB XB WL HWL QC 15 You can modify the patient demographic data or add a test to an existing sample To add a test select a new profile The pr...

Page 159: ...les can be collated You must preassign the sample for multiple tests You can either 1 Preassign both tests on the Worklist or 2 Assign another test to an existing sample result in Data base by editing...

Page 160: ...hrs of the first test 2 RBC votes out 3 RBC is incomplete 4 RBC is overrange 5 RBC has been edited E 6 Flow cell is clogged affects Diff and Retic results 7 Insufficient data for Retic results 8 Diff...

Page 161: ...Data Base Query Select sample on Data Base Query list Press Edit demog profile Change profile to include test to be collated Run pending test Manual Collation Sort CBC or CBC Diff sample from Data Bas...

Page 162: ...50 05 05 93 12 55 00 OPR R F S 1538 05 05 93 12 56 45 OPR R F S 1538 05 05 93 12 58 52 OP Batch Process S 4290 05 05 93 12 59 44 OP Batch is Inactive S 430 05 05 93 13 00 55 OP Samples left 0 S 4310 0...

Page 163: ...sents a choice list for the Archive field No New All Resume Resume batch processing after you have used Abort to stop the process This function resumes batch processing where it left off when stopped...

Page 164: ...2 On the sorted list tag samples you want to archive Tag to tag individual samples highlighted by cursor to tag all samples on sorted list Data Base Query Saved 0 Entries 10 10 Tagged 0 Cass pos ID 1...

Page 165: ...ntil the Batch is Inactive message appears in the Batch Process window Removing the disk sooner can cause disk corruption 7 Wait until the Batch is Inactive message appears then remove the disk from t...

Page 166: ...spiration 2 No Match 3 No Read 4 Mismatch 5 Collate failure Example Status 0 1 5 0 3 WBC Population RBC Population Plt Population 0 Normal 1 Abnormal 2 Edited Example WBC Pop 0 0 1 2 All Suspect and D...

Page 167: ...e the data base the last sample run remains in the buffer and is displayed when you go to the Run Sample screen The buffer sample disappears after the next cycle occurs When you delete the data base s...

Page 168: ...DATA BASE 158 PN 4237188A December 1993...

Page 169: ...s Start Up Special Functions DMS 2A Diagnostics Set Up Control set up et up C CBC DIFF Files Latex Files CBC Files Retic Files Expected Range oooooooooooo oooooooooooo CBC DIFF Files oooooooooooo CBC...

Page 170: ...d Retic controls it does not apply to latex controls When the Auto Stop option is ON the Analyzer stops if The control file is not found CBC DIFF and Retic controls only The control file is full The c...

Page 171: ...ime Out CBC Diff Retic 932422 COPYRIGHT 1989 1993 COULTER CORPORATION ALL RIGHTS RESERVED F1 Help F4 Print F6 Clear F10 Save Esc 09 04 93 09 10 OPR DMS PR TK HC DB XB WL HWL QC 49 The Interlaboratory...

Page 172: ...stics Set Up Control set up Sample analysis set up C System set up Shift Time Range nts tution 1 07 00 14 59 nication def ronic reference 2 15 00 22 59 age ID ooooooo 3 23 00 06 59 CBC Diff Retic 9312...

Page 173: ...TUP retic latex 107351 NOT SETUP latex 3 107351 NOT SETUP abnormal low 620 NOT SETUP abnor high 821 NOT SETUP NOT SETUP NOT SETUP NOT SETUP NOT SETUP NOT SETUP NOT SETUP NOT SETUP NOT SETUP CBC Diff R...

Page 174: ...ected Range 2 5 40 2 0 F1 Help F2 Choice lists F4 Print F9 Menu F10 Save Esc 06 05 93 11 56 OPR DMS PR TK HC DB XB WL HWL QC 32 The LEVEL field was automatically filled in when you entered the lot num...

Page 175: ...n must be read first 3 Gently slide the wand back and forth over the bar code until you hear a beep The lot number is now in the Lot field on the CONTROL SET UP screen If the bar code wand does not re...

Page 176: ...ple Analysis Controls Start Up Special Functions DMS 2A Diagnostics Set Up Control set up et up C CBC DIFF Files Latex Files F55 DIFF only F56 Retic only oo F57 DIFF Retic oo oooooooooooo oooooooooooo...

Page 177: ...p CBC Files Select File Sample Analysis Controls Start Up Special Functions DMS 2A Diagnostics Set Up Control set up et up C CBC DIFF Files Latex Files CBC Files Retic Files Expected Range Auto Stop o...

Page 178: ...T field A window appears with the shift selections that were entered in the Shift Set Up mode Select the shift you want and press In the HOST field press to toggle setting to ON or OFF If AutoTransmit...

Page 179: ...meter RET Computed Value 0 0450 Computed Limit 0 0090 F1 Help F2 Choice lists F4 Print F9 Menu F10 Save Esc 06 01 93 08 39 OPR DMS PR TK HC DB XB WL HWL QC 41 Enter the lot number and press For Retic...

Page 180: ...that all entries are correct press to save them HOW CELL CONTROLS ARE PROCESSED There are four ways a cell control can be processed All four apply to 5C cell control The third and fourth apply to Ret...

Page 181: ...tch Current control file Discard Primary Mode with control bar code ID not read or no bar code label or Secondary Mode with a non control ID Current control file Data base QC STATUS The QC field is at...

Page 182: ...ilable Unable to archive file You must acknowledge these conditions by pressing A control ID is either a bar code label or a number entered on the Diluter keypad If the bar code label is read or the a...

Page 183: ...with this lot number but the shift number does not match If the currently displayed screen is a CBC DIFF or RETIC CONTROL RUN the run data is saved to that file Otherwise the control data is lost Exam...

Page 184: ...ol run results is out of the expected value range Control run data is saved in the file Example CONTROL FILE 4 Abnormal I IS OUT OF RANGE Control Expired The file exists and is not full but the contro...

Page 185: ...number and shift already exists Example Lot number shift are duplicated in another file A control file with the same lot number and shift 0 24 hour shift already exists Example The same lot number wi...

Page 186: ...ile 1 Run number 50 No Data Available Unable to archive control file There is no floppy disk in drive A The floppy disk is not formatted Example Unable to archive CONTROL file1 WKS The floppy disk ins...

Page 187: ...READ YES NO STOP DISPLAY FILE NOT FOUND CURRENT SCREEN IS CONTROL RUN YES NO DATA BASE FILE IS FULL YES NO DISPLAY FILE IS FULL PROCESS CONTROL RUN SAVE CONTROL RUN IN CONROL FILE NUMBER OF RUNS LEFT...

Page 188: ...rwise results are lost 1 Review file setup 2 Correct error 3 Rerun control Control Expired System Continues or Auto Stop optional Results in Control file 1 Review file setup 2 Verify Lot Exp date Corr...

Page 189: ...9 93 10 14 OPR 9 0 5 69 16 7 49 2 86 3 29 2 5 09 30 93 12 10 OPR 9 3 5 66 16 6 49 4 87 3 29 4 6 09 30 93 12 11 OPR 9 0 5 65 16 6 49 1 86 8 29 4 7 10 01 93 09 06 OPR 9 2 5 62 16 7 48 7 86 6 29 7 8 10 0...

Page 190: ...OPR DMS PR TK HC DB XB WL HWL QC 50 Move the cursor to the file you want and press DIFF LATEX CONTROL REVIEW FILE 3 F57 DIF RETC EXP DATE 07 25 94 IQAP ID 75007 LOT 107366 Mean Channel CV RUN DATE TI...

Page 191: ...graphs appear To review single parameter histograms of Volume Conductivity Scatter and Opacity from either the DIFF or RETIC LATEX CONTROL RUN screen or the DIFF or RETIC LATEX CONTROL REVIEW screen...

Page 192: ...15 9 4 38 13 9 48 0 91 4 31 7 34 7 14 1 326 9 3 2SD 15 4 4 29 13 5 0 5 0 3 0 4 0 5 0 2 5 0 CV 0 5 0 2 0 6 0 7 0 8 N 6 6 6 5 5 5 5 5 6 6 ASSAY 19 2 5 23 16 6 47 8 91 4 31 7 34 7 13 8 389 10 4 LIMITS 0...

Page 193: ...ew Retic control graphs go back to the Control menu window set the cursor to Graphs and press To view graphs of a different file press select the file you want and press BATCH PROCESSING FOR CONTROLS...

Page 194: ...ove the cursor to the type of files you want to process and press 2 Use to toggle the Print Host and Archive processes to Yes No 3 For Review Report you can set the Date From and To fields to batch pr...

Page 195: ...spirations are not included in X B Analysis When a batch of 20 samples has been collected the DMS performs the X B Analysis and calculates the batch mean of MCV MCH and MCHC Use the Run Sample screen...

Page 196: ...e sample s values for the other parameters are used If a sample s RBC value is less than 1 x 106 the DMS automatically excludes that sample from the calculations The total number of deletes in a batch...

Page 197: ...3 The AUTO REPORTER 3 has a bar code reader that reads labels on the tickets and sends the label data to the DMS The DMS sends back the corresponding results and the AUTO REPORTER 3 prints the result...

Page 198: ...eves the corresponding results from its data base and sends them to the AUTO REPORTER 3 where they are printed If there is no bar code label on the ticket or if the AUTO REPORTER 3 cannot read the lab...

Page 199: ...eration of the AUTO REPORTER 3 various messages may appear on the front panel display Table 13 lists the messages their causes and the required operator action Table 13 Messages on AUTO REPORTER 3 Dis...

Page 200: ...ry test has found a bad location 1 Record the chip number Uxx 2 Call Coulter Customer Operations Waiting for data The AUTO REPORTER 3 is in the HOPPER mode Data is sent from the DMS via but there is a...

Page 201: ...int the ticket M manually printed ticket IMPORTANT If the last sample cycled has not yet been printed by the AUTO REPORTER 3 its results are stored in the DMS Sample Analysis buffer When you insert a...

Page 202: ...3 A sample with an ID 1 goes from the Analyzer to the DMS the DMS compares the sample to the data base If the DMS finds more than one sample in the data base with that ID and at least two have not had...

Page 203: ...the sort section of the drawer back and forth and automatically ejects the ticket TO REPRINT A TICKET IMPORTANT If the last sample cycled has not yet been printed by the AUTO REPORTER 3 its results a...

Page 204: ...APPENDIX A 194 PN 4237188A December 1993...

Page 205: ...bes Special cassettes are provided for the HEMOGARD tubes You can put both the regular cassettes and the special cassettes in the loading area at the same time The special cassette can hold tubes with...

Page 206: ...wn from the cap Figure shows how to position a bar code label on the new tubes with HEMOGARD Closure and on the regular tubes with a modified system 0 5 REGULAR TUBES LABEL 0 5 IN AWAY FROM CAP BARS P...

Page 207: ...rmed stained film differentials For recognition criteria for cell type reference refer to Reference Leukocyte Differential Count and Evaluation of Instrument Methods H20 A Section 5 Peripheral Blood N...

Page 208: ...opulations with low numbers Rumke 1978 The uncertainty of reference value recovery is a direct function of the number of cells counted in the differential cell count Depending upon the acceptable rang...

Page 209: ...A TOTAL TOTAL GRAPH POINT SERIAL NO STKS 1 REF 1 STKS 2 REF 2 2 GRAPH POINT CYCLE NE LY MO EO BA 1 2 TECH NE LY MO EO BA NE LY MO EO BA NE LY MO EO BA CYCLE 1 2 TECH MONTH DAY YEAR 1 2 1 2 STKS 1 2 2...

Page 210: ...nd Graph Points for Five Days Day STKS 1 Ref 1 STKS 2 Ref 2 Graph Point 1 53 5 51 5 46 0 45 5 1 25 2 49 0 50 0 72 5 73 5 1 0 3 60 0 62 0 44 5 43 0 0 25 4 63 5 61 0 60 0 60 5 1 0 5 43 0 46 5 56 5 58 0...

Page 211: ...0 1 2 3 4 5 6 7 2SD 1SD 1SD 2SD 7 6 5 4 3 2 1 0 1 2 3 4 5 6 7 MO 2SD 1SD 1SD BA EO 4 3 2 1 0 1 2 3 4 4 3 2 1 0 1 2 3 4 4 3 2 1 0 1 2 3 4 SERIAL NO ____________________ 2SD 2SD 1SD 1SD 2SD 2SD 1SD 1SD...

Page 212: ...tages of Blood Cells as Determined By Differential Counts Observed n 100 n 200 n 400 n 800 1 0 3 0 2 0 2 0 2 2 0 5 0 4 1 3 1 3 3 0 6 1 5 1 5 2 4 4 0 8 1 7 2 6 3 5 5 1 9 2 8 3 7 3 7 6 1 11 3 9 4 8 4 8...

Page 213: ...43 27 41 29 39 31 37 35 26 44 28 42 30 40 32 38 36 26 46 29 43 31 41 33 39 37 27 47 30 44 32 42 34 40 38 28 48 31 45 33 43 35 41 39 29 49 32 46 34 44 36 42 40 30 50 33 47 35 45 37 43 41 31 51 34 48 3...

Page 214: ...62 68 66 57 75 59 73 61 67 63 69 67 58 76 60 74 62 72 64 70 68 59 77 61 75 63 73 65 71 69 60 78 63 75 64 74 66 72 70 61 79 64 76 65 75 67 73 71 62 80 65 77 67 75 68 74 72 63 81 66 78 68 76 69 75 73 64...

Page 215: ...0 94 82 92 84 90 85 89 88 82 94 83 93 85 91 86 90 89 83 95 85 93 86 92 87 91 90 84 96 86 94 87 93 88 92 91 85 97 87 95 88 94 89 93 92 87 97 88 96 89 95 90 94 93 88 98 89 97 90 96 91 95 94 89 99 91 97...

Page 216: ...APPENDIX C 206 PN 4237188A December 1993...

Page 217: ...and manufacturers Typically do not pierce a blood collection tube listed in this document more than five times The Universal Tube Processing Module consists of Universal Button Stripper Plate Accommo...

Page 218: ...lass Tubes with HEMOGARD Closure BD No Volume o d x Length mm Cassette 367650 367651 367653 367658 367661 367662 3 0 mL 2 0 mL 5 0 mL 5 0 mL 3 0 mL 5 0 mL 13 0 x 75 13 0 x 75 13 0 x 75 13 0 x 75 13 0...

Page 219: ...713 5 0 mL 5 0 mL 12 0 x 75 12 0 x 75 Special for tubes with HEMOGARD Closure Can be used with or without the Cassette Clips installed in this cassette SHERWOOD MEDICAL SHERWOOD No Volume o d x Length...

Page 220: ...th rubber stopper caps or foil tops Place the bar code label so that the first bar of the bar code symbol is at least 1 2 inch from the tube cap For all other tubes Place the bar code label so that th...

Page 221: ...ight gray List of Tubes You must use the following tubes with the gray sleeve adaptors BECTON DICKINSON VACUTAINER Tubes Worldwide BD No Volume o d x Length mm Adaptor Cassette 6384 6385 2 0 mL 3 0 mL...

Page 222: ...either side of the bar code symbol are visible through the read slit when you insert the tube into the adaptor Place tubes with bar code labels into the two types of sleeve adaptors 2 mL and 3 mL so t...

Page 223: ...code read slits fit into the top openings of the cassette 7225002B TOP OPENING READ SLIT KEY TUBES WITH CASSETTE CLIPS Permanently place the Cassette Clips into all 12 openings of each special casset...

Page 224: ...ter recommends that you use these tubes with the Cassette Clips installed in the special cassette for tubes with HEMOGARD Closure GREINER Vacuette Tubes GREINER No Volume o d x Length mm Cassette with...

Page 225: ...acturer s label Do not skew the bar code label more than 12 degrees Ensure that the bar code symbol and a 1 4 inch blank space quiet zone on either side of the symbol are visible to the scanner Place...

Page 226: ...ft counterclockwise until it clicks into place Then push down on the Cassette Clip to position it at the bottom of the cassette opening Note It is easiest to insert the first Cassette Clip into the fa...

Page 227: ...essing Module is present Note If the button portion of the stripper plate is stainless steel then you do not have the Universal Tube Processing Module on your system Variations of this stripper plate...

Page 228: ...APPENDIX D 218 PN 4237188B March 1995...

Page 229: ...iaz DD Quinn PG Lapins M Kurtz SR and Valeri CR The Role of Anticoagulant in the Measurement of Platelet Volumes AJCP 1983 80 327 332 4 NCCLS document H3 A3 Procedures for the collection of diagnostic...

Page 230: ...REFERENCES 220 PN 4237188B March 1995...

Page 231: ...uto stop option 160 autopurge 90 Auto Reporter 3 ticket 56 B backwash message 3 bar code labels 54 rule for Interleaved 54 sample tubes 55 ticket 56 Tubes 60 bar code wand 165 batch processing 152 bub...

Page 232: ...on verify 14 Diluter keypad 4 distribution curve Plt 75 RBC 75 DMS startup 9 DMS keys 105 drain key 4 14 E electronic manometer 1 13 entering ID numbers 39 error conditions 172 error messages for cont...

Page 233: ...tion condition 82 83 85 positive identifiers 123 power on key 4 power supply checks at startup 12 controls and indicators 8 startup 12 precision of results 79 Premix key 5 press manual 22 prime when t...

Page 234: ...e 108 QC 171 status messages on Analyzer CRT 3 STKS initializing message 3 Stop key 5 storing specimens 52 stripper plate release key 5 suspect messages 84 85 switching between cell classification off...

Page 235: ...B 3 95 Change pages cover inside front cover iv vi viii 47 51 85 109 131 136 167 186 195 207 217 221 trademarks and back cover Note Changes that are part of the most recent revision are indicated in t...

Page 236: ......

Page 237: ...ISOPET ISOTERGE ISOTON LANGLEY FORD LANGLEY FORD INSTRUMENTS LEASE PAK LFI logo LYSE S MDADS MINI KEM NANO SIZER OMNISORP OptiChem S CAL SOMACOUNT SOMAFIX SOMATON STAIN RIGHT THROMBOCOUNTER THROMBO F...

Page 238: ...ce Adjust Procedures Troubleshooting Operator s Guide PN 4237188 Clear binding Controls and Indicators Startup Sample Analysis Data Analysis Shutdown Analyzer CRT Functions DMS Basics Sample Analysis...

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