1
(English)
EN
Issue Date: 2015-08
Product Instructions
Select
E. coli
Count Plate
Product Description And Intended Use
The 3M™ Petrifilm™ Select
E. coli
Count (SEC) Plate is a sample-ready-culture medium system which contains
selective agents, nutrients, a cold-water-soluble gelling agent, and an indicator of glucuronidase activity, 5-bromo-
4-chloro-3-indolyl-D-glucuronide (BCIG), which facilitates colony enumeration. 3M Petrifilm SEC Plates are used for
the enumeration of
Escherichia coli (E. coli)
in the food and beverage industries. Most
E. coli
strains are thermotolerant
and produce beta-glucuronidase, an enzyme that reacts with BCIG indicator in 3M Petrifilm SEC Plates to produce
dark green to blue-green colonies. 3M Petrifilm SEC Plate components are decontaminated though not sterilized. 3M
Food Safety is certified to International Organization for Standardization (ISO) 9001 for design and manufacturing. 3M
Petrifilm SEC Plates have not been tested with all possible food products, food processes, testing protocols or with all
possible strains of
E. coli
or other bacteria.
Safety
The user should read, understand, and follow all safety information in the instructions for the 3M Petrifilm SEC Plate.
Retain the safety instructions for future reference.
WARNING:
Indicates a hazardous situation, which, if not avoided, could result in death or serious injury and/or
property damage.
CAUTION:
Indicates a hazardous situation, which, if not avoided, could result in minor or moderate injury and/or
property damage.
W
WARNING
Do not use 3M Petrifilm SEC Plate for the detection of
E. coli
O157. Like most other
E. coli
media, the 3M Petrifilm
SEC Plate will not specifically indicate whether any O157 strain is present. Because most O157 strains are
atypical
E. coli
, for example they are glucuronidase negative and they will not produce a blue-green color, they
will not be detected on 3M Petrifilm SEC Plate.
To reduce the risks associated with release of contaminated product:
• Follow all product storage instruction contained in the instructions for use.
• Do not use beyond expiration date.
To reduce the risk associated with bacterial infection and workplace contamination:
• Perform 3M Petrifilm SEC Plate testing in a properly equipped laboratory under the control of a skilled
microbiologist.
• The user must train its personnel in current proper testing techniques: for example, Good Laboratory Practices
1
, ISO
17025
2
, or ISO 7218
3
.
To reduce the risks associated with misinterpretation of results:
• 3M has not documented 3M Petrifilm SEC Plates for use in industries other than food and beverage. For example,
3M has not documented 3M Petrifilm SEC Plates for testing water, pharmaceuticals, or cosmetics.
• Do not use 3M Petrifilm SEC Plates in the diagnosis of conditions in humans or animals.
• 3M Petrifilm SEC Plates do not differentiate any one
E. coli
strain from another.
• High sugar content foods may decrease the color reaction of the glucuronidase and produce pale green to colorless
colonies.
To reduce the risks associated with environmental contamination:
• Follow current industry standards and local regulations for disposal of contaminated waste.
CAUTION
A few strains of bacteria other than
E. coli
, such as some
Shigella
,
Salmonella
,
Enterobacter
and
Klebsiella
, can produce
beta-glucuronidase and will produce dark green to blue-green colonies on the 3M Petrifilm SEC Plate, especially when
incubated at lower than recommended temperatures
4
.
Consult the Safety Data Sheet for additional information.
