ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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8 • Troubleshooting
Page 160 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Message and Code Description and Remedial Action
Code 8049 Command rejected - close
front door first It is not possible to start data acquisition with the front doors open.
Close the doors and try again.
Code 8051 Flow cell detected. It is
recommended executing the
command CalibrateRaman A new flow cell was found. It is recommended to perform a Raman
wavelength calibration (see
Performing a Wavelength Calibration
(
}
page 132) ); without calibration, wavelengths may deviate from the
actual wavelength by up to a few nanometers.
Code 8064 PMT x signal overflow in
<channel name>
Code 8065 PMT x signal overflow
during scan
With x = PMT 1 or 2
Signal overflow in PMT 1 or 2 occurred. Repeat the run or scan with a
lower sensitivity or lower concentration of the sample.
Code 8071 PMT Sensitivity autorange
for <channel name> -new Sensitivity
is x With x = The new sensitivity value after the adjustment.
The detector had to automatically reduce the Sensitivity. Select a
suitable Sensitivity (see
Sensitivity (Detector Sensitivity) ( } page 108) )
and repeat. x indicates the new sensitivity value after the adjustment.
Use this value, or a smaller value, in your method when you expect
similar concentrations.
Code 8078 PMT Autorange for
<channel name> not possible -
already minimal Sensitivity The detector could not automatically reduce the Sensitivity, as the
Sensitivity setting is already set to a minimum. Repeat with a lower
concentration of the sample, or smaller injection volume.
Code 8087 System Interlink error The System Interlink connection got lost. Check that all System
Interlink cables on the Vanquish system are properly connected.
Code 8093 Not initialized The detector is not initialized. Verify that a flow cell is installed
correctly and that the eluent composition in the flow cell does not
change and no air bubbles are present. Then open and close the
doors, or press
INIT on the keypad.
Code 8100 - Code 8109 Calibration of
xx failed. With x = Name of the component for which calibration failed.
Cause details
• Raman calibration failed.
• The flow cell may be installed incorrectly.
• The flow cell may be contaminated.
Remedial actions
• Observe the conditions for wavelength calibration (see Performing a
Wavelength Calibration ( } page 132) ) and wavelength validation
(see Performing a Wavelength Validation (
}
page 134) ).
• Verify that the flow cell is installed correctly and that the screws are
tightened finger-tight.
• Clean the flow cell (see Cleaning the Flow Cell ( } page 138) ) if
necessary.
• Make sure that the flow cell is properly equilibrated. Make sure that
you use degassed solvents.
If the procedure is repeatedly interrupted, contact Technical Support.