6
•
Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Page 119
6.10.2 Long-Term Shutdown
Shutting Down the Device
To interrupt operation for a longer period, follow the instructions below.
TIP
Shutting down the device affects the operation of the system. When
shutting down the device, also observe the shutting down instructions
for the other Vanquish system modules and take appropriate action
(refer to the
Operating Manuals
for the modules).
1. Stop the pump flow.
2. Remove the column.
3. Connect the free ends of the column compartment capillaries using
a union connector (for example, the Viper union from the system
ship kit) and restart the pump flow.
4. Flush the flow cell with an appropriate solvent (minimum HPLC-
grade). Observe the following:
TIP
With a Vanquish Core system that has been modified for using
normal-phase compatible solvents and additives, see the information
about the flushing liquid in the
Considerations with Normal-Phase
Compatible Solvents and Additives
section in
Operating Manual
for the
pump.
Situation after Shutdown
If no additive is used
If an additive is used
Device and flow cell
remain in the laboratory
after shutdown
Flush the system, for
example with methanol.
100% acetonitrile should
not be used.
Flush the system with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the flow cell are not
miscible with water, use
an appropriate
intermediate solvent.
Содержание Vanquish VC-D50
Страница 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 154: ...7 Maintenance and Service Page 154 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Страница 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...
Страница 188: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com...