ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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6 • Operation
Page 110 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Determining the optimum sensitivity
Determine the optimum sensitivity in a separate sample run after you
have determined the optimum wavelengths as described below.
• Use a sample (standard) with the maximum expected concentration
of the analytes.
• Select a sensitivity at which a saturation is not expected (for
example, 1 or 2).
• If the peak heights of the individual peaks in a chromatogram differ,
you can improve the signal-to-noise ratio of the smaller peaks by
switching the sensitivity during the run and between the peaks with
the help of a timetable.
• Always use the same PMT setting for determining the sensitivity that
is used for the analysis.
An instrument method example is available in the Chromeleon Help .
Follow these steps
1. Open the method you want to optimize in the Script Editor . The
required commands must be added to the method manually.
2. Add a ClearMaxPMTSaturation command at the beginning of the
run.
3. Add a Log command for the MaxPMTSaturation property after the
peak maximum of an expected peak (or group of peaks) to log the
maximum saturation value at this time in the Audit Trail (see figure
below).
4. Add a ClearMaxPMTSaturation command shortly after, before the
next peak elutes, to reset the MaxPMTSaturation value to zero (see
figure below).