ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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6 • Operation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 111
Figure 34: MaxPMTSaturation parameter monitoring
5. Repeat steps 3 through 4 for all peaks.
6. Run a sample and read out the MaxPMTSaturation values in the
Chromeleon software and evaluate the results:
Value What you should do…
< 30% Increase the Sensitivity. The following is a rough guideline:
< 30 %: by one stage
< 15 %: by two stages
Then repeat the optimization, starting with step 1.
30% - 80% The Sensitivity value is optimal.
80% - 99% The Sensitivity should be reduced by one stage to prevent unexpected
saturation when concentration varies.
≥ 100% Reduce the Sensitivity by at least one stage. In most cases, the detector
has reduced Sensitivity automatically ("autoranging"). Check the Audit
Trail for the smallest Sensitivity value after the autoranging and repeat
the optimization by using this value, starting with step 1.
If you do not know the expected concentrations of the analytes, reduce
the sensitivity even at smaller values of
MaxPMTSaturation .
TIP
• The maximum saturation indicated by MaxPMTSaturation always
refers to the signals of both PMTs at the parameter settings for all
channels.
• You can completely turn off fluorescence detection by setting the
sensitivity to
Off . This may be required, for example, if you want to
record the incoming signal from a UV detector for a sample of high
concentration, or to suppress an extremely high peak that is not of
interest for you but triggers an automatic adjustment of the
sensitivity.