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ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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6

 • 

Operation

Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51) 
Operating Manual

Page 97

•

Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example

¨

Column compartment and post-column cooler

¨

Sample compartment thermostatting in the autosampler

¨

Flow cell in a fluorescence detector

¨

Evaporation tube in a charged aerosol detector

•

Turning on the lamp (or lamps) in the UV/VIS detector

•

Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application

•

Monitoring the detector signal and checking whether the detector
signal is stable so that the drift and signal noise are within
reasonable limits for the application

•

Performing an autozero of the detector baseline

TIP

 The Chromeleon software supports procedures for automatically

starting a chromatography system in the software (

Smart Startup

). The

startup procedure includes the operations for system equilibration. For
details, refer to the 

Chromeleon Help

.

Содержание Vanquish VC-D50

Страница 1: ...thermoscientific Vanquish Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual 4820 7901 EN Revision 2 0 December 2019...

Страница 2: ...ded as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or e...

Страница 3: ...sales organization For contact information go to Contact Us on http www thermofisher com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific suppo...

Страница 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 5: ...1 3 Safety Symbols on the Device 19 2 1 4 Rating Plate 19 2 2 Intended Use 20 2 3 Safety Precautions 21 2 3 1 General Safety Information 21 2 3 2 Qualification of the Personnel 21 2 3 3 Personal Prote...

Страница 6: ...4 5 6 1 General Information and Guidelines 64 5 6 2 Guiding Capillaries and Tubing Through the System 65 5 6 3 Installing the Partition Panel Plugs 67 5 6 4 Connecting Fittings Capillaries and Tubing...

Страница 7: ...g the Performance of the Device 106 6 9 1 General Guidelines 106 6 9 2 Overview of Optimization Parameters 107 6 9 3 Determining the Optimum Excitation and Emission Wavelength 107 6 9 4 Sensitivity De...

Страница 8: ...9 Updating the Device Firmware 142 7 10 Replacing the Doors 144 7 11 Transporting or Shipping the Device 146 7 11 1 Preparing the Device for Transport 146 7 11 2 Transporting the Device to a New Locat...

Страница 9: ...formation 172 10 2 Ship Kit 173 10 3 Optional Accessories 174 10 4 Consumables and Replacement Parts 175 11 Appendix 177 11 1 Compliance Information 178 11 1 1 Declarations of Conformity 178 11 1 2 WE...

Страница 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 11: ...C D50 VC D51 VF D50 VF D51 Operating Manual Page 11 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documentation t...

Страница 12: ...t can prevent personal injury damage to the device or loss of data when followed properly Note the following The device configuration may vary therefore not all descriptions necessarily apply to your...

Страница 13: ...rticular section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or alert...

Страница 14: ...rograms Thermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are identif...

Страница 15: ...er or Adobe Acrobat is required Software Documentation Additional software documentation includes the following Chromeleon Help and documents The Chromeleon Help provides extensive information and com...

Страница 16: ...0 VF D51 Operating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers of th...

Страница 17: ...Safety Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 17 2 Safety This chapter provides general and specific safety information and informs about the intended use of the devi...

Страница 18: ...ng CAUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided could result in serious injury 2 1 2...

Страница 19: ...ly is on Power supply is off Indicates alternating current Indicates that the surface becomes hot during operation Do not touch these surfaces while they are heated up 2 1 4 Rating Plate The rating pl...

Страница 20: ...uish system are intended to be used as General Laboratory Equipment GLE They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the laborato...

Страница 21: ...d for the device by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual and in supporting documents for the device Follow all instructions step by step and...

Страница 22: ...esented by the device and the substances they are using All users should observe the related Safety Data Sheets SDSs 2 3 3 Personal Protective Equipment Wear personal protective equipment and follow g...

Страница 23: ...l or grounding connections If you suspect any kind of electrical damage disconnect the power cord and contact Thermo Fisher Scientific Technical Support for assistance Do not open the housing or remov...

Страница 24: ...harmful substances Make sure that the installation site is well ventilated Dispose of hazardous waste in an environmentally safe manner that is consistent with local regulations Follow a regulated app...

Страница 25: ...ydrofuran THF can cause PEEK to swell Concentrated acids such as sulfuric acid and nitric acid or a mixture of hexane ethyl acetate and methanol can attack PEEK Swelling or attack is not a problem wit...

Страница 26: ...2 Safety Page 26 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual 2 3 6 In Case of Emergency WARNING Safety Hazard In case of emergency disconnect the device from the power line...

Страница 27: ...llowed pH Ranges Allowed pH ranges standard system configuration System Standard Configuration Allowed pH ranges Remarks Vanquish Core 1 13 pH values of 2 or less The application time should be as sho...

Страница 28: ...exposure Vanquish Horizon Vanquish Flex 1 mol L or less 2 4 4 Further Information For details about the materials that are used in the analytical flow path of the device see the Specifications chapte...

Страница 29: ...hanges that you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or peripherals not...

Страница 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 31: ...3 Device Overview Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 31 3 Device Overview This chapter introduces you to the device and the main components...

Страница 32: ...detectors under Chromeleon 7 software A wavelength switching time 250 ms Optimized detection for high sensitivity signal to noise ratio higher than 550 for the Raman spectrum of water at 350 nm excita...

Страница 33: ...e returns to its ground state it emits light at a higher wavelength emission The photomultiplier tube PMT is positioned at an angle of 90 to the light source and detects the light that was emitted fro...

Страница 34: ...ures the intensity of the excitation light The reference signal is used to compensate fluctuations in lamp intensity and thus improve sensitivity Upon exiting the flow cell the emitted light is focuse...

Страница 35: ...light passes the flow cell to the reference sensor the fluorescence light exits the flow cell at an angle of 90 to the excitation light 5 Reference sensor Measures the excitation light that passes th...

Страница 36: ...rs Figure 4 Interior view here with flow cell installed No Description 1 Cooling air intake 2 Keypad with status indicators 3 Flow cell 4 Leak tray with leak sensor 5 Partition panel The recesses in t...

Страница 37: ...block Do not touch the optical block Optical ports Flow Cell Label One or more flow cell labels are present on the flow cell which contain information such as flow cell type part number and serial num...

Страница 38: ...ume of the heat exchanger and inlet capillary influences the retention times and peak widths Flow Cell Types All flow cells are optimized for fast separations with no loss in chromatographic resolutio...

Страница 39: ...frequency of the lamp varies depending on the selected lamp mode Selecting a different lamp mode during phases when no peaks of interest elute can extend the lamp lifetime You can monitor the lamp age...

Страница 40: ...liquid leaks from the flow connections The liquid is collected in the leak tray and guided to the drain port From the drain port the liquid is discharged to waste through the drain system of the Vanq...

Страница 41: ...omated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help TIP The device can...

Страница 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 43: ...Unpacking Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 43 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of deliver...

Страница 44: ...bulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a te...

Страница 45: ...and place it in the system stack see System Arrangement page 54 7 On each carrying handle loosen the attachment screw until the carrying handle is moveable in the rail Do not remove the screws from t...

Страница 46: ...VF D51 Operating Manual 4 2 Scope of Delivery The following items are included in the delivery Detector Ship Kit Operating manual Power cord For information on contents of the ship kit or reordering p...

Страница 47: ...VC D51 VF D50 VF D51 Operating Manual Page 47 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the device in the Vanqu...

Страница 48: ...A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Nev...

Страница 49: ...ng the system modules as described in the Vanquish System Operating Manual 1 Pay attention to the safety guidelines and observe all site requirements See Safety Guidelines for Installation page 48 and...

Страница 50: ...em refer to the documentation for the software that you are using and or perform the qualification manually The Instruments Installation Qualification Operating Instructions provide information about...

Страница 51: ...ing Manuals for the other modules in the Vanquish system For general residual hazards see General Residual Hazards page 23 5 3 1 Power Considerations The power supply of the device has wide ranging ca...

Страница 52: ...vice from the power line WARNING Electric Shock or Damage to a Product Misuse of the power cords could cause personal injury or damage the instrument Use the power cords provided by Thermo Fisher Scie...

Страница 53: ...ge 53 5 4 Accessing the Interior Components To access the interior components in the device open the front doors To allow easy access from the front the user accessible components and flow connections...

Страница 54: ...m modules are typically arranged in a system stack with the arrangement depending on the system configuration The following illustrations show configurations with a single fluorescence detector and wi...

Страница 55: ...0 VC D51 VF D50 VF D51 Operating Manual Page 55 System with Single Detector Figure 10 Vanquish system standard configuration example No Description 1 Solvent Rack 2 Fluorescence detector 3 Autosampler...

Страница 56: ...D51 Operating Manual System with Fluorescence Detector as Second Detector Figure 11 Vanquish system configuration with two detectors example No Description 1 Solvent Rack 2 Fluorescence Detector 3 UV...

Страница 57: ...fuse rating and the manufacturer s address 2 Main power switch on off control 3 Fuse holder 4 Power inlet connector 5 System Interlink port Allows power on off control for the detector from the Vanqu...

Страница 58: ...installed such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above If the USB ports are used for any other purpose Thermo Fisher Scientific c...

Страница 59: ...erating Manual The Detector is the Second Detector in the System If the fluorescence detector is the second detector in the Vanquish system for example after the diode array detector set up the USB an...

Страница 60: ...D51 Operating Manual Figure 13 Cable connections in the Vanquish system with fluorescence detector and diode array detector example No Description 1 System base 2 Pump 3 Sampler 4 Diode array detector...

Страница 61: ...low cell upon initial installation of the detector For instructions on removing a flow cell or installing a flow cell after storage see Flow Cell page 136 NOTICE Flow cells are highly sensitive to dir...

Страница 62: ...emoved TIP Keep the cover to close the flow cell opening when no flow cell is installed in the device especially when the detector is transported or shipped 2 Unpack the flow cell Figure 14 Flow cell...

Страница 63: ...cence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 63 Follow these steps 1 Insert the flow cell straight into the flow cell opening Figure 15 Inserting the flow cell 2 Tighten the flow...

Страница 64: ...waste before connecting them in the system flow path To flush the Vanquish modules follow the instructions in the Vanquish System Operating Manual NOTICE Flow cells are highly sensitive to contaminat...

Страница 65: ...the flow cell see Flow Connections to the Flow Cell page 71 2 Connect the device to the drain system refer to the Vanquish System Operating Manual For installation instructions guidelines and handlin...

Страница 66: ...cket side onto the drain pipe Figure 17 Tubing bracket left tubing bracket installed right Dual System Arrangements The number of tubes may exceed the capacity that the tubing guides can hold In this...

Страница 67: ...mall outer diameter such as uninsulated capillaries 2 Rotating plug for guiding capillaries that do not fit in the plug with slit including insulated capillaries Installing the plug with slit 1 On the...

Страница 68: ...ry through the plug turn the rotating plug toward the front 3 To secure the capillary turn the rotating plug toward the partition panel to close the opening of the rotating plug Figure 21 Using the ro...

Страница 69: ...d to invalid test results Do not install capillaries or tubes that are stressed nicked kinked or otherwise damaged Install capillaries and fittings only at the positions for which they are intended 5...

Страница 70: ...ons you can easily remove the knurls from neighboring capillaries through this slot and attach them again later 3 Check whether the connection leaks If leakage exists follow the steps further down Res...

Страница 71: ...ne When connecting a component in the flow path after the flow cell observe the specified backpressure for the flow cell In addition observe the guidelines for proper connection of the flow cell in Ge...

Страница 72: ...r Waste Line page 75 5 6 5 1 Connecting the Inlet Capillary Depending on the modules in your Vanquish system you can connect the inlet capillary directly from the column compartment or from a previous...

Страница 73: ...hrough the bottom recess in the partition panel Make sure that you use the suitable partition panel plug for the capillary TIP For capillaries with small outer diameter the plug with slit secures the...

Страница 74: ...Figure 24 Connecting the inlet capillary from the diode array detector example 1 Connect the inlet capillary from the column compartment to the flow cell inlet of the first detector Refer to the instr...

Страница 75: ...t of the fluorescence detector Make sure that you secure the inlet capillary appropriately in the capillary clip on the front panel 5 6 5 2 Connecting the Detector Waste Line Preparations 1 See Flow C...

Страница 76: ...ne should go straight to the system base and to waste Make sure that the line is positioned straight in the tubing guides 5 If you have to cut tubing to length use a tubing cutter Make sure that the c...

Страница 77: ...er the flow cell Depending on the application for example if you use high salt concentrations this procedure may help you troubleshooting Parts required Union connector for example Viper union from th...

Страница 78: ...ulating the Pressure at the Inlet Port of the Flow Cell page 84 Flow path Pump autosampler column flow cell additional module waste line WARNING Escape of Hazardous Substances from Flow Connections Fl...

Страница 79: ...er inlet capillary from the pump static mixer outlet port 3 Connect the waste line to the pump static mixer outlet port 4 Insert the other end of the waste line into the waste container 5 Start the pu...

Страница 80: ...f the flow cell 6 Second detector 2b Inlet port of the flow cell 7 Waste line 3 Column 8 Detector inlet capillary 1 If applicable disconnect the detector inlet capillary from the flow cell IN port 2 I...

Страница 81: ...measure the backpressure of the additional module including that of the column detector inlet capillary and waste line p2 Afterward the backpressure of the transfer capillary and the additional modul...

Страница 82: ...Chromeleon and write down the value for p2 p2 Pressure drop at the transfer capillary and the additional module including the column detector inlet capillary and waste line 8 Stop the pump flow 9 Cal...

Страница 83: ...ste line No Description No Description 1 Detector 3 Detector inlet capillary 2 Flow cell 4 Waste container 2a Outlet port of the flow cell 5 Waste line 2b Inlet port of the flow cell 1 Set up the flow...

Страница 84: ...lculating the Pressure at the Inlet Port of the Flow Cell This procedure describes how to determine the pressure at the flow cell inlet port p6 for the intended configuration Figure 31 Calculating the...

Страница 85: ...ure drop at the waste line 2 Compare p6 with the pressure limit of the flow cell Situation Steps p6 is at least 5 bar below the pressure limit of the flow cell The configuration can be used p6 is less...

Страница 86: ...system can detect the device To turn on the power to the device follow these steps 1 Check that the power button on the front left of the Vanquish system base system power button is pressed in If the...

Страница 87: ...that the chromatography software is already installed on the data system computer and a valid license is available For more information about setting up the Vanquish system in the software refer to t...

Страница 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 89: ...tion Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 89 6 Operation This chapter describes the elements for device control provides information for routine operation and for s...

Страница 90: ...this is not the case refer to the instructions in Installation page 47 For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanquish Sy...

Страница 91: ...tatements presented in Safety Precautions page 21 CAUTION Hot Surfaces Surfaces inside the device may become hot during operation Touching hot parts might cause burns Allow hot surfaces to cool down b...

Страница 92: ...tatus of the device 6 3 1 Keypad The keypad inside the device allows you to perform certain functions directly from the device When you press a button a short beep confirms that the function is perfor...

Страница 93: ...e keypad provide information about the device status LED Bar The LED bar provides the information when the device is closed When the device is connected in the Chromeleon software the LED bar may prov...

Страница 94: ...the keypad inside the device provides the following information STATUS LED Description Off dark The power to the device is turned off Green The device is functioning properly Red A problem or error ha...

Страница 95: ...power switch on a device is off you cannot turn on the device with the system power button To turn off a device completely you have to turn it off with the main power switch on the device Pressing the...

Страница 96: ...When operating the device with a flow cell that was stored the flow cell may be filled with solvent Use solvents that are miscible with this solvent or use an appropriate intermediate solvent Before S...

Страница 97: ...p or lamps in the UV VIS detector Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application Monitoring the d...

Страница 98: ...om the flow cell opening in the detector and replace the flow cell Interrupted operation If the pump flow is interrupted take appropriate measures to protect the flow cell If data acquisition is still...

Страница 99: ...ode for very small peaks 6 7 2 Multi Channel Mode VF detectors only In multi channel mode both monochromators quickly switch between the selected wavelengths A single analysis is sufficient to measure...

Страница 100: ...th page 107 6 7 3 Zero Order Mode In Zero Order Mode the grating of the emission monochromator reflects the entire emission spectrum of the sample onto the PMT rather than only a single wavelength The...

Страница 101: ...nded stopping the pump flow or have the pump deliver at a very low flow rate If the scan can be completed during the elution of a peak and the scan is significantly faster than the full width at half...

Страница 102: ...uration of the scan process depends on the range to be scanned and the selected scan speed Before each scan select a detector sensitivity using the ScanSensitivity parameter If the detector sensitivit...

Страница 103: ...Field Acquisition is available in the Chromeleon 7 software only Use a 3D data field to determine the retention times and optimum emission or excitation wavelengths For more information about 3D data...

Страница 104: ...e Device page 106 Excitation wavelength Sets the excitation wavelength in nm Select the excitation wavelength at the absorption maximum of the sample component to be analyzed Emission wavelength Sets...

Страница 105: ...sic calibration by determining the start position of the grating motors and filter wheel Execute if the message Not initialized appears in the Chromeleon Audit Trail after the detector is turned on Le...

Страница 106: ...le with the specific application In many cases selecting fluorescence grade solvents should serve the need for low background fluorescence However your normal HPLC grade solvents may also be suitable...

Страница 107: ...el setting Baseline noise permitted wavelength range for emission PMT Emission wavelength range and therefore excitation wavelength range Response time Baseline noise peak width peak height Data colle...

Страница 108: ...fter completion of the wizard open the method in the Instrument Method Editor In the Module View for the detector on the Timetable tab page you can define the times for switching the wavelengths and o...

Страница 109: ...Figure 33 Effects of the Sensitivity setting With Vanquish fluorescence detectors the intensity of the emission light measured by the PMT is normalized with the intensity of excitation light through...

Страница 110: ...during the run and between the peaks with the help of a timetable Always use the same PMT setting for determining the sensitivity that is used for the analysis An instrument method example is availabl...

Страница 111: ...one stage In most cases the detector has reduced Sensitivity automatically autoranging Check the Audit Trail for the smallest Sensitivity value after the autoranging and repeat the optimization by us...

Страница 112: ...an the cut off wavelength is blocked In practice there is a transition area where the transmission of the light increases from the lower wavelengths near the range where the light is cut off to the hi...

Страница 113: ...entire spectral range is recorded For further information about selecting the filter wheel setting refer to the Fluorescence Method Development Handbook that is shipped with the device 6 9 6 PMT only...

Страница 114: ...disk space and post run analyses may require more processing time If all peaks are relatively wide select a lower data collection rate for example 1 0 Hz This saves disk space and allows for a faster...

Страница 115: ...e decreases as temperature increases Therefore the temperature must not be selected too high However it must be above the temperature inside the detector optics which is influenced by the ambient temp...

Страница 116: ...o the Fluorescence Method Development Handbook HighPower Mode The lamp flashes at the highest frequency of 300 Hz The sensitivity is roughly twice the sensitivity in Standard mode The expected lifetim...

Страница 117: ...vior mode settings define how the baseline behaves in these cases Zero sets the baseline to zero Append appends the baseline to the previous signal Free lets the baseline jump to the current absolute...

Страница 118: ...te the following Detector Type Description Charged aerosol detector Check that sufficient gas is available to continue gas flowing through the detector This is to prevent any build up of residue from...

Страница 119: ...he pump flow 4 Flush the flow cell with an appropriate solvent minimum HPLC grade Observe the following TIP With a Vanquish Core system that has been modified for using normal phase compatible solvent...

Страница 120: ...tion solvents containing acid can damage the flow cell Always flush the flow cell with an appropriate solvent before interrupting operation Fill the flow cell with pure isopropanol using the flushing...

Страница 121: ...Restart after Long Term Shutdown To restart the device after a long term shutdown follow these steps 1 Prepare and restart the other modules in the Vanquish system following the instructions in the O...

Страница 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 123: ...and Service Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 123 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the us...

Страница 124: ...ntific for brevity referred to as Thermo Fisher Scientific service personnel The device is designed for easy maintenance and service The user serviceable parts of the device can be accessed from the f...

Страница 125: ...nces that can pose health risks Solvent can spray when capillaries burst slip out of their fittings or are not properly tightened or when capillary connections are otherwise open Wear appropriate prot...

Страница 126: ...the pump flow and wait until the system pressure is down to zero Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from the reservoirs Empty the solvent li...

Страница 127: ...e tools recommended for the procedure Before opening the flow path to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components can c...

Страница 128: ...ain buffers or salts Monitor the backpressure that the flow cell is exposed to Regularly Inspect the flow connections for damage such as cracks nicks cuts or blockage Perform operational qualification...

Страница 129: ...Avoid open flames or exposure to excessive heat during the cleaning process Wipe the cleaned components thoroughly dry after cleaning Do not operate the device before it is completely dry NOTICE Obse...

Страница 130: ...ice and qualification information about the device Monitoring service and qualification intervals On special service and qualification panels you can define intervals for service procedures or qualifi...

Страница 131: ...he calculation of the expected lamp lifetime one operating hour is weighted depending on the selected lamp mode Mode 1 operating hour is counted as Expected lamp lifetime LongLife 0 25 hours approx 16...

Страница 132: ...r If wavelength validation fails Preparations The following conditions must be met for both wavelength validation and Raman wavelength calibration The system is equilibrated and environmental conditio...

Страница 133: ...rating Manual Page 133 2 Confirm with OK if you are sure that the above conditions are met The calibration run may take up to five minutes The new calibration values are stored in the detector 3 Perfo...

Страница 134: ...libration The system is equilibrated and environmental conditions are stable There are no air bubbles in the flow cell We recommend degassing the water Set a flow rate of 0 5 mL min and wait about 15...

Страница 135: ...calibration of the detector is not sufficient Perform a wavelength calibration If wavelength validation fails again after the recalibration check the causes and remedial actions outlined below Possib...

Страница 136: ...e flow cell be careful when inserting the flow cell into the flow cell opening of the device On the rear side of the flow cell the contact pads for the identification chip are located Never touch the...

Страница 137: ...w cell inlet and outlet with the flow cell plugs that were installed when the flow cell was shipped 3 Loosen the two flow cell screws until they are loose The screws are captive in the flow cell and d...

Страница 138: ...risks Wear personal protective equipment as required by the hazard and follow good laboratory practice Refer to the material handling and safety data sheet provided by the vendor Follow these steps Yo...

Страница 139: ...e 37 Inserting the flow cell 2 Tighten the flow cell screws hand tight 3 Install the capillaries to the flow cell Follow the instructions in Setting Up the Flow Connections page 64 Turn on the flow an...

Страница 140: ...ages are present inside the device that could cause an electric shock or damage to the device Turn off the device with its main power switch Disconnect the power cord from both the power source and th...

Страница 141: ...141 1 Use the screwdriver to remove the fuse holder 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconnect th...

Страница 142: ...leon software 3 Stop all operations on the Instrument that includes the device 4 Wait until the Instrument is idle Follow these steps 1 Start the Instrument Configuration Manager program 2 Perform a f...

Страница 143: ...ee whether the firmware update was successful or failed If the firmware update failed turn the device off and on again and repeat the firmware update If the firmware update fails repeatedly contact Th...

Страница 144: ...nt door Follow these steps NOTICE To avoid damage to the door hinges be careful when performing the following sequence of steps and do not apply force 1 To remove a door push the door upward while ope...

Страница 145: ...the door align the door with the hinges on the housing Be careful not to clamp tubing or capillaries between the door and the enclosure 4 Insert the hinges in the groove by pushing up and slightly tur...

Страница 146: ...ticular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the dev...

Страница 147: ...pping the System section of the Vanquish System Operating Manual or Remove the slide in module from the device enclosure in the system stack see Removing the Slide In Module page 149 See also 2 Removi...

Страница 148: ...us substances may have contaminated the device during operation and may cause personal injury to service personnel Decontaminate all parts of the device that you want to return for repair Fill in and...

Страница 149: ...e device into the system stack or when removing it Tools required Screwdriver Torx T20 Preparations 1 Prepare the device for transport See Transporting or Shipping the Device page 146 Follow these ste...

Страница 150: ...Take the slide in module on both sides from below b Pull the slide in module from the rails towards the front c Place the slide in module on a clean and stable surface 7 12 2 Returning the Slide In M...

Страница 151: ...Heavy Load Bulky Device The device is too heavy or bulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of th...

Страница 152: ...ke the slide in module on both sides from below b Lift the slide in module to the height of the enclosure c Place the slide in module in the enclosure so that the module sits in the enclosure by appro...

Страница 153: ...the slide in module in the enclosure set up and restart the device Follow these steps 1 Set up the slide in module a Connect the slide in module and set up flow connections see Installation page 47 b...

Страница 154: ...7 Maintenance and Service Page 154 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 155: ...oubleshooting Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 155 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the de...

Страница 156: ...the keypad inside provide quick visual feedback on the operational status of the device If the device firmware detects a problem the status indicators are red The problem is reported to the Chromeleon...

Страница 157: ...D51 VF D50 VF D51 Operating Manual Page 157 Messages in the Chromeleon Audit Trail are preceded by an icon The icon identifies the seriousness of the problem refer to the Chromeleon Help For possible...

Страница 158: ...ode Description and Remedial Action Unexpected module behavior Code xx xx Two digit to four digit code number When the message appears write down the message code and turn off the module Wait for 5 se...

Страница 159: ...o third party software on the computer for example virus scanners can cause the problem Contact the onsite IT department Code 120 The request timed out Communication with the module failed The module...

Страница 160: ...ar concentrations Code 8078 PMT Autorange for channel name not possible already minimal Sensitivity The detector could not automatically reduce the Sensitivity as the Sensitivity setting is already se...

Страница 161: ...ctly and that the screws are tightened finger tight Clean the flow cell see Cleaning the Flow Cell page 138 if necessary Make sure that the flow cell is properly equilibrated and no air bubbles are pr...

Страница 162: ...s at a connection However leakage may also have occurred inside the flow cell Location of the leak Steps Flow cell inlet and or outlet 1 Tighten the connection where liquid is visible 2 If the connect...

Страница 163: ...troubleshooting for the Vanquish system refer to the Vanquish System Operating Manual TIP If you are unable to resolve a problem following the instructions given here or if you experience problems th...

Страница 164: ...of the fluorescence detector was automatically reduced Select a lower Sensitivity for your application High baseline drift Absorption of eluent changes when gradient is run Absorbing additives may cha...

Страница 165: ...ce Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 165 9 Specifications This chapter provides the physical and performance specifications including information about the materials used in...

Страница 166: ...nm Emission 265 nm 650 nm Number of photomultipliers 1 VC D51 Excitation 200 nm 880 nm Emission 265 nm 900 nm Number of photomultipliers 2 VF D50 Excitation 200 nm 630 nm Emission 220 nm 650 nm Number...

Страница 167: ...alibration excitation monochromator with emission lines of xenon flash lamp emission monochromator with Raman shift of water and emission lines of xenon lamp Wavelength validation Internal validation...

Страница 168: ...he Chromeleon software 9 1 2 Flow Cells The flow cell performances are specified as follows Type Standard Flow Cell Micro Flow Cell Flow cell volume 8 L 2 L Volume heat exchanger and or inlet capillar...

Страница 169: ...re 5 C 35 C Ambient storage temperature 20 C 45 C Ambient operating humidity 20 80 relative humidity non condensing Ambient storage humidity Maximum 60 relative humidity non condensing Operating altit...

Страница 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...

Страница 171: ...1 Operating Manual Page 171 10 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as...

Страница 172: ...only with the replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts are al...

Страница 173: ...ded in the kit for the most recent information about the kit content at the time when the device is shipped Ship kit Item Quantity in shipment Partition panel plug for guiding insulated capillaries 1...

Страница 174: ...valve Suitable to protect the Micro flow cell of the fluorescence detector against overpressure The valve opens at a pressure of 4 MPa 40 bar 6079 9240 TIP UltiMate 3000 fluorescence detector flow cel...

Страница 175: ...uding right door and left door 6083 3018 Fuses kit Vanquish system The kit includes the appropriate fuses for the Vanquish system modules For the detector use only 3 15 AT 250 V AC slowblow fuses 6036...

Страница 176: ...t Parts Page 176 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Description Part No Power cord Italy 6000 1040 Power cord Japan 6000 1050 Power cord UK 6000 1020 Power cord USA 60...

Страница 177: ...Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 177 11 Appendix This chapter provides additional information about compliance UV cutoff wavelengths and the use of the digital...

Страница 178: ...certain hazardous substances in electrical and electronic equipment The CE mark on the device indicates that the product is compliant with the directive China RoHS regulations Measures for Administrat...

Страница 179: ...l device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment Thi...

Страница 180: ...y also contain salts such as sodium hydroxide NaOH Most solvents have a UV cutoff wavelength within the spectral range of the device For optimum measurement results perform quantitative measurements a...

Страница 181: ...input Input 5 Relay output Relay_COM COM is the common contact for NO and NC If the relay is not activated or if the device is turned off the connection is between COM and NC If the relay is activate...

Страница 182: ...following prerequisites must be fulfilled The digital I O port must be connected to the external device with the digital I O signaling cable part no 6036 0006 The inputs and outputs that you want to...

Страница 183: ...tandby 118 Smart Startup 97 cleaning 128 condensation 52 61 connectors 57 consumables 171 175 cTUVus mark 178 cutoff filter 112 D data collection rate 107 114 guidelines 114 decontamination 128 129 de...

Страница 184: ...lines 48 site requirements 51 system 49 intended use 20 interior view 36 Interlink 57 K keypad 92 L lamp 39 131 lamp mode 115 replace 131 lamp age 131 lamp lifetime 131 lamp mode 107 leak detection 40...

Страница 185: ...149 reordering 171 replacement parts 171 175 reproducibility 107 response time 107 114 restart device after long term shutdown 121 RoHS mark 178 S safety class 21 safety glasses 22 safety guidelines...

Страница 186: ...2 93 156 STATUS LED 92 93 156 synchronous scan 102 system arrangement 54 system equilibration 96 System Interlink 57 T temperature control 38 transport 146 troubleshooting 155 general information 156...

Страница 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...

Страница 188: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com...

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