ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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7 • Maintenance and Service
Page 134 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
7.6 Performing a Wavelength Validation
You can validate the wavelength calibration using water (Raman
measurement). If validation fails, that is, if the measured values
significantly deviate from the calibration values, you can perform a
wavelength calibration using water (Raman measurement). The light
spectrum of the xenon flash lamp is used to validate the excitation
wavelength.
When
• After (re)installation of the detector
• After wavelength calibration
Preparations
The following conditions must be met for both wavelength validation
and Raman wavelength calibration:
• The system is equilibrated and environmental conditions are stable.
• There are no air bubbles in the flow cell. We recommend degassing
the water. Set a flow rate of 0.5 mL/min and wait about 15 minutes
before you begin, until the baseline is stable.
• The water has no impurities. Always use fluorescence-grade water
and consider using a filter or a suitable column before the detector.
To perform a wavelength validation
You can perform wavelength validation via Chromeleon. Wavelength
validation can take a few minutes. During this time, data acquisition is
not possible.
1. Execute the ValidateRaman command to start the validation.
The following message appears in Chromeleon:
Make sure that
¨
the system is equilibrated and conditions are stable
¨
fluorescence-grade water is flowing through the cell
¨
the water is degassed (no air bubbles) and free of particles