GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 60
Touching “Auto Adjust” will have the system automatically calculate and apply a compensation
percentage (image below – bottom/right red arrow and box).
The compensation value can be fine-tuned (or manually adjusted) by touching the arrows (red
bracket in image above – below/right) as follows:
•
Double arrows – change the compensation percentage up/down by 1%
•
Single Arrows – change the compensation up/down by 0.1%
Note: The system waits for a break in the tapping of the up/down arrows before applying the
new value (refreshing) the scatter plot data on the screen. This allows the user to tap the
arrows multiple times to quickly adjust the value without having to wait for a refresh with each
press.
The amount of compensation (as a percentage of the corresponding signal on the
primary PMT, e.g. 525/45nm in this case, image above - below/right) is displayed in the white
box (e.g. 10.0% in this example). The scatter plot is also updated to show the compensation
effect with the value shown in red text after the axis label (see red box in image above –
bottom/right). Also note the relative shift in
the spillover cluster position (as well as the
yellow box
D
MFI value) in the images
above for uncompensated (image above -
bellow/left, 0% compensation) to the fully
(561nm/LP channel) compensated data
(image above - below/right, 10.0%
compensation). The characteristic
spillover slant has been eliminated and the
height of the spillover cluster (on the y-axis)
is visually the same as the control cluster.
This can also be confirmed by the updated
D
MFI value of (-0.23mV, essentially no
difference in PMT2 fluorescence).
The image to the right shows compensation
examples for data. The left column
represents the initial, uncompensated data.
The right column represents the result of
compensation. The first and second rows
show the bead compensation example first
on the PMT2 (spillover channel) vs size
view (row 1) and then on the PMT2
(spillover channel) vs PMT1 quadrant view.
In both cases, you can see from the right
column that the signal on the spillover
channel (PMT2) is reduced to being equivalent to the dark bead cluster (it’s at the same height
Uncompensated Compensated