Moxi GO II
™
User Guide
Page 34
Reading Raw Data Output (Conc., Size, MFI, and %)
The Moxi GO II™ provides particle concentration and precise sizing information for each gated
region, total counts information, fluorescent percentages, and median fluorescent intensities (all
where applicable based on the channels selected) for each test. Three example test view
screens are shown above (left to right: Fluorescence vs. size scatter (dot) plot, Single Channel
Histogram Display, Fluorescence vs. Fluorescence Scatter (Dot) plot).
Fluorescence vs. size scatter (dot) plot
Image above/left:
For this plot, statistics are only generated for the size-gated (between the
blue markers), noise excluded (points that aren’t grayed out following a test) region. The total
concentration (provided as particles/ml) is listed above the scatter plot between the blue gate
region (1.924 x 10
5
cells/ml in the example above/left). The two black boxes below the scatter
plot provide the subset concentration (cells/ml), mean particle diameter (
Note: This is an
“effective” diameter that is based on a precise volumetric measurement (Coulter Principle)
combined with a spherical assumption.
), particle volume (Coulter-Principle measured), and
median fluorescence intensity (MFI) values for the regions below (“Lower population”) and
above (“Upper Population”) the red fluorescent gate (but within the size-gated region). The
percentage of events that fall above/below the fluorescence (red) gate is displayed in the yellow
box above the scatter plot (e.g. 39.4% in this example).
Single Channel Histogram Plot
Image above/middle:
An example of the on-unit histogram display for the PMT2 (Long Pass
channel) Only the points within the size-gated region for the corresponding fluorescence
channel vs. size plot (e.g. Long Pass (PMT2), image above right) are included in generating a
histogram. This is deliberately done to avoid having noise contributions in the histogram. The
red and green shading are determined by the positioning of the fluorescence (red) gate marker
on that same fluorescence vs. size plot (image above/left) with the red representing the
fluor points (above the red line) and the green representing the fluorescence- (below
the red line) points. Any areas of overlap in the histogram (between red and green populations)
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