GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 46
of saved events) to correspondingly increase the storage capacity of the disk in terms of number
of tests and images that can be saved. This was more significant with the v2.09 and earlier
releases of the firmware that were limited to 16Mb storage (vs. 4Gb+ with v2.10 firmware). For
almost all tests, it is sufficient to leave this gate, unadjusted, at the system-specified noise
exclusion setting.
“Web” or “Quadrant” Gating – Fluorescence vs. Fluorescence Scatter Plots
In analyzing tests with two recorded fluorescent channels, it is usually desirable to plot one
fluorescent channel vs. the other fluorescent channel to identify the four possible quadrants of
data (as defined by the permutations of positive and negative for each channel: PMT1
+
/PMT2
-
,
PMT1
+
/PMT2
+
, PMT1
-
/PMT2
+
, PMT1
-
/PMT2
-
). The gating for fluorescence vs. fluorescence
(PMT vs PMT) displays is presented with a quadrant or “Web” gating interface (see images