GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 16
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It is best to place the pipette facing straight into the system (not angled left or right)
and a ~45degree angle (up/down, see image below)
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Dispense the fluid in one, smooth motion as you would normally during pipetting.
The system does apply a slight vacuum to pull the fluid it, but it is not recommended
to try to “time the load” to the draw of the fluid. It is normal/expected to have a bead
of fluid form over the entry well. That fluid will get pulled in eventually.
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The system provides the user a 60 second window to complete each step (opening
the door, loading the sample, waiting for the fluid to reach the start electrode). If the
time limit for any of the steps is exceeded, the test will automatically cancel and the
user will be returned to the
Home screen.
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The instrument will begin the test. While the system waits for sample/fluid detection an
“Analyzing…” message is indicated in top left of the screen a countdown until the test is
auto-cancelled (auto cancel occurs after 60 seconds if no fluid is detected). Once the
fluid is detected, the system message changes to “Counting…” and the results are
dynamically populated on the scatter/dot plot. Results for most samples will be complete
in approximately 10 seconds but may be longer for higher concentration, sticky, or
aggregated samples.
Please refer to the “Test Results – Analyzing/Gating Data”
section for details on gating and interpreting the data output for all tests.
“Open Flow Cytometry” Test
The Open Flow Cytometry is meant to be a generic test assay that can be used to run any user
sample, whether or not it has been prepared with reagents obtained from Orflo. The
requirement for labeling of the sample is that the fluorophore used is compatible with the 488nm
laser excitation and 525/45nm and/or either 561nm/LP or 646nm/LP (depending on which the
user has installed) filter sets. For non-Orflo kits, it might be necessary to perform titration
assays and/or adjust the Fluorescent Gain (Very Low, Low, Default, Medium, or High) to
achieve desirable test results.
1. As with all assays, please follow sample preparation guidelines above (“Sample
Preparation Considerations”) in preparing the sample. Note: The minimum volume
required for a test is 60 µL.