GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 7
Cells need to be prepared as single-cell suspensions. Clusters/aggregates should be broken
apart with mechanical trituration (e.g. pipetting vigorously) and/or protease dissociation (e.g.,
Accutase
®
or Accumax
®
). For stubbornly aggregated samples, pre-filtration with a 40 µm cell
strainer is recommended.
As the Moxi GO II™ uses a 488 nm laser with 525/45 nm and either (561nm/LP or 646nm/LP,
user-swappable) detection filters, dye/fluorophore selection should be based accordingly. A few
examples for each include:
PMT Filter Compatible Fluorophore Examples (not complete list)
525/45 nm FITC, Alexa Fluor
®
488, BB515, GFP, Calcein, Acridine Orange,
BODIPY 500/510, Rhodamine 110
561nm/LP PE, PI, PE-Cy5, PerCP-Cy5, 7-AAD, PerCP, DsRed
646nm/LP PI, 7-AAD, PE-Cy5
For assays other than “Open Flow Cytometry” and “GO Flow”, using ORFLO optimized and
validated protocols are strongly recommended. Please see
(
http://www.orflo.com/v/vspfiles/pages/protocols.html ) for a list of currently available protocols.
For cell counting application, cells can be run without fluorochromes using any of the assays.
Additional Materials Required
•
Cell or bead sample (diluted and dissociated, if necessary); 60 µL minimum.
•
Type S+ (MXC030, recommended for almost all assay) or MF-M (MXC010) Cassettes
•
Pipette (typically P100 or P200) and appropriately sized pipette tips
•
Cell staining/labeling reagents (test specific, see guidelines in “Sample Preparation”)