GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 39
“Cell QC” – Output (both Single Run and Batch)
An example of the Initial Cell QC “Results Summary” is shown in the image above/left. This
initial screen is designed to provide a simple representation of all the cell/sample data based on
the system auto-gating of the PMT2 (Propidium Iodide, Viability) vs Size scatter plot (more detail
below) to identify the core cell population (by size) and determine the viability (by PI
fluorescence). The user is provided the opportunity to review the system gate placement by
touching the “Back” button. Touching that button brings up the standard PMT2 (Propidium
Iodide) vs. Size scatter plot (example above/right). Gates can be adjusted by touching and
dragging the markers (blue/size, red/fluorescence, yellow/noise) as described in the “PMT vs.
Size Gating” section of this User Guide. It is recommended that users verify that gate for the
initial test/run with any new sample/cell types. Once verified, the user can simply refer to the
simplified test output screen. The user can return to the simplified output/summary screen by
touching the orange “Data Summary” button under the scatter plot.
The “Results Summary” screen has the test information at the very top. If this test is part of a
batch, the “Batch Name” will be added to that summary (as shown above). The Dilution Factor,
selected by the user during test configuration, is automatically applied to the concentration data
provided below.
Some notes on the data:
•
The key information, dilution-adjusted concentration and viability, are highlighted in the
white box.
•
The “Debris %” is calculated as the particle counts in the range from 3µm (minimum
detectable particle size) to the left size gate (start of the cell region).
•
The “Cell Counted” is the absolute number of cells in the size-gated region, based on the
22.6µL metered test volume.
•
The histogram shows the live cell population in green, the dead cell population in red,
and the overlapping regions in yellow.