GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 48
Histogram Overlays on Fluorescence vs Size Scatter Plots
Fluorescent and Size histograms overlays on the scatter/dot plot can be toggled on and off by
pressing the blue histogram button at the bottom right of the scatter plot (see images below).
With histogram overlays turned on, the fluorescent histogram appears as a blue histogram with
a base on the left (fluorescent) axis (blue dashed lines point to the histograms in the images
below). The size histogram is displayed as a red histogram with a base on the bottom (size)
axis. The histograms can be rescaled by swiping your finger in the appropriate direction: Up-
down for the size histogram and left-right for the fluorescent histogram. The image below/right
shows the effect of making broad left-to-right swipes as well as top-to-bottom swipes in the
scatter plot region of the middle image below. The result is that the fluorescence histogram
scale is decreased (increasing histogram height) and the size histogram scale is increased
(shrinking the size histogram).
“Size Histogram” Assay
For samples that lack any staining or fluorescent label, quick counts and particle sizing
information can be obtained by running a “Size Histogram” assay. As the laser is not used with
this assay, time is saved as the system skips the laser/cassette alignment step. Size-only tests
can be run by selecting “Cell Counts (Size Only)” assay. During acquisition a size-based
histogram is displayed as opposed to the traditional fluorescence vs. size scatter (dot) plot.
Upon completion of the test, counts for the “Size histogram” can be obtained by moving the blue
gating markers to define the size region for the counts (image below/middle). For monodisperse
cell populations, the system can also be set to curve-fit the data automatically. When curve-
fitting, the system looks to find the core, singlet Gaussian population. That population is curve-
fit automatically. The system uses the mean of the Gaussian to determine (and report) the
singlet cell size/volume (black box in the bottom right).
For smaller particle populations, improved size resolution can be achieved by rescaling the x-
axis (as discussing in the above “Rescaling the X-axis” section) to 2-17 µm or 2-9 µm.
Rescaling doesn’t just show a subset of the currently displayed histogram. Rather, system
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