Moxi GO II
™
User Guide
Page 11
On entering the passcode, the user will be required to “Re-enter Passcode” to prevent
accidental entry of a wrong code.
Upon successful re-entry of the passcode, the system will display a “lock enabled” message.
The system will now require a passcode for access on initial startup and after entering sleep
mode.
NOTE: In order to turn the Passcode Lock off, the user will be required to enter the
correct passcode. If you forget this passcode, the entire system will have to be reset and
data could be lost. Keep the passcode recorded in a safe place to prevent loss of data.
Fluorescence Gain
The “Fluorescence Gain” field sets the level of fluorescence gain for the “Open Flow Cytometry”
and “GO Flow” assays only. Five possible values (
Very Low,
Low, Default, Medium, High
) can
be entered here and will seed the value of the dropdown list on the “Open Flow Cytometry” and
“GO Flow” start screens. To change the values simply tap on the current value and the system
will toggle the gains with each tap. Note: the user can also make a one-time (per-test) change
to the fluorescent gain value of the “Open Flow Cytometry” and “GO Flow” assays by touching
the value on the test start screen as described in “Running a Test” “Open Flow Cytometry Test”
section (step 6).
Auto Noise-Find
This field sets the behavior of the system with respect to identifying system noise during tests.
There are two possible settings:
Yes
or
No
. If set to
Yes
, the system applies an algorithm to
each test, after acquisition, to try to identify the noise (and small-particle “debris”) in the sample.
If set to
No
, the system will use a hard-coded values for the noise location for each test, capping
the noise area to a region defined by events <2.9µm AND fluorescence levels <10mV.
With the
v2.10 (or later) release of the firmware, it is recommend that this setting is left in the “Off”
setting.
Auto Gate settings
The user is given control in setting the system auto-gating to one of three settings:
1.
Off
– the system will not auto-gate any tests.
2.
Viability Assays
– the system will auto-gate the Viability tests only: Cell QC, PBMC
Check, CAR T Expansion and Cell Count (Size + Viability).
3.
All Assays
– the system will auto-gate the first PMT vs. Size display output for all tests.
To toggle the auto-gate value, simply tap on the current setting for that field.
Sample Preparation Considerations
There are several key considerations for preparing a sample for analysis with the Moxi GO II™:
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