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Moxi GO II
™
User Guide
Page 37
The PMT2 (Propidium Iodide, viability) vs size scatter plot is display (above/middle). The
placement of the size gates (blue markers, vertical lines, two top dashed red arrows in image
above/middle) and fluorescence gate (horizontal red line, two lower dashed red arrows in image
above/middle). The gates, size scale, and axis display can all be adjusted as described in the
“Manual Gating – PMT vs Size Gating” section. Briefly, gates are moved by touching and
dragging the markers (blue for size gates, red for fluorescence gate, yellow for noise gate). The
gate mode (size, fluorescence, noise) can be toggled by touching the red icon at the top/right of
the plot. The RBC gates, used for the RBC contamination calculation, can also be accessed by
touching the green “RBC Gates” icon (above/middle). The blue size gate positions (dashed red
arrows, image above/right) are displayed. The range of the RBC gate can be adjusted by
touching and dragging the blue icon (more detail in the “Manual Gating – PMT vs Size Gating”
section).
“CAR-T Expansion" Output
The initial, simplified output of the CAR-T test is shown below. This output is based on the
system’s auto-gating of the PMT2 (Propidium Iodide, Viability) vs Size scatter plot (more detail
below) to identify the core CAR-T population (by size) and determine the viability (by PI
fluorescence). The core information is provided at the top with total PBMC count (e.g. 250,398
cells/ml in the image below) and Viability (e.g. 52%, image below). Further detail on the
statistics (concentration, cell diameter, cell volume, and median fluorescence intensity) are
provided below for both viable (green box, image above) and dead cell (orange box, image
above) populations. The user can save, print a screenshot (bitmap), or delete the test by using
the “File button at the bottom left. Touching the button brings up the three options.
Содержание MXG102
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