GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 30
Test Results
Recorded Channels
The Moxi GO II system has the capability of recording the following channels of data for each
sample:
•
Particle Size
•
PMT1 Fluorescence (525/45nm filtered, e.g. FITC, Alexa 488, GFP, Calcein, et.c)
•
PMT2 Fluorescence (561nm/LP filtered, e.g. PE, or 646nm/LP filtered, e.g. PI)
•
Forward Extinction (“Absorbance”)
Data Display Formats – General Output Types
Certain tests (PBMC Check, CAR T Expansion, and Cell QC) provide simplified data outputs,
presenting just the essential test information based on auto-gating algorithms implemented by
the system. For more detail on interpreting/reading those test outputs, please refer to the
appropriate section for that test in this User Guide. Other tests, will immediately display the raw
data for the user view to directly. This data may-or-may-not be auto-gated as well, depending
on the system “Auto Gate Setting” setting (see “settings” section).
Note: All test results are
ultimately based on the gating of the raw data displays. Even with the simplified tests, the user
always has the option to view/review the raw data and adjust the gates if needed.
The raw data can be displayed in three main formats: 1.). Histograms (one channel display –
any channel) – Values for each event are binned across a 1000 point histogram and displayed.
2) Fluorescence vs. Size scatter plots (all fluorescent assays) – For each event, the recorded
PMT millivolt level (range 0-10,000mv, corresponding to fluorescence intensity on that channel)
is plotted against the recorded cell size (effective diameter in microns). This forms a 2D scatter/
dot plot 3.) Fluorescence vs. Fluorescence Scatter plots (two fluorescence channel assays
only). On these plots the recorded PMT millivolt level (range 0-10,000mv, corresponding to
fluorescence intensity on that channel) on one PMT channel is plotted against the other PMT
channel (or Absorbance).
Detailed labeling for each of the three types of display are provided on the three pages/images
below. After that, an explanation of how to read the key statistics (e.g. counts, sizes, and
percentages) from those data displays is provided.