GeminiBio MXG102, Руководство пользователя

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Moxi GO II
™
User Guide
Page 45
fluorescent gating line. All events/dots that are located above the fluorescent gating line (and
within the blue size gates) are included in the “Upper Population” statistics (images below, black
box to the right underneath the scatter plot). Those events/dots below the line (and within the
blue size gates) are included in the “Lower Population” statistics (images below, black box to the
left underneath the scatter plot).
The general recommended placement is to place the fluorescence gate in the midpoint between
the dark (non-fluorescent or noise) clusters and the bright (fluorescent) cluster. Often, this
placement can be facilitated by by turning on the histogram overlays (blue button at the bottom
right of the scatter plot) and viewing the peaks. The red fluorescent gate can then be put in the
valley of that distribution. If multiple fluorescent peaks are not present, the recommendation is
to place the fluorescent gate at the top/tail of the noise cluster. Gate placement can also be
faciliated by running control (negative or positive) tests, setting the gates (above or below the
control, respectively), storing the gates locations (see section on storing/recalling gates), and
then recalling the gates for the standard, labeled sample.
Noise Gating of Scatter (Dot) Plots
Touching the red gate toggle marker when the fluorescent (red) markers are currently enabled
correspondingly disables the (red) fluorescence gate markers and enables the (yellow) noise
gate markers.
Note: The noise gate is only available after a test is run, before it is saved. It is
not available for saved data. The user can then touch and drag the yellow circle (images below)
to define the points that will be identified as noise. These noise points will be grayed out (image
below/middle) and will not be saved with the test.
Note: DATA GATED AS NOISE WILL NOT
BE SAVED WITH YOUR FILE!! Additionally, data in the noise region will not be included in
the stats (counts, sizes, percentages, etc). For example, referring to the image below/right, note
that the lower bead cluster (~6µm), while in the size region and below the red fluorescence gate
line, is not included in the Lower Population counts (i.e. those counts are all zero). This is
because that cluster was included in the noise region during noise gating (below/middle). The
primary purpose of the noise gate was to reduce the saved file size (by eliminating the quantity