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PN B05529AE

2-1

CHAPTER 2

Operation

Introduction

This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors

designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing

the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power

turned off

(

O

)

when the centrifuge is not in use.

NOTE

If the centrifuge is used in a manner other than that specified in this manual, the safety and

performance of this equipment could be impaired.

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Содержание Allegra X-30 Series

Страница 1: ...PN B05529AE September 2018 Instructions For Use Allegra X 30 Series Centrifuges Rx Only Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A ...

Страница 2: ... services are trademarks or registered trademarks of their respective holders Contact Us If you have any questions contact our Customer Support Center Worldwide find us via our website at www beckman com support technical In the USA and Canada call us at 1 800 369 0333 Outside of the USA and Canada contact your local Beckman Coulter Representative Beckman Coulter Eurocenter S A 22 rue Juste Olivie...

Страница 3: ...n of the manual or system help for your instrument Issue AD 11 2015 Changes were made to Introduction CE Mark Issue AE 09 2018 Changes were made to CHAPTER 1 Available Rotors Note Changes or additions that are part of the most recent revision are indicated in text by a bar in the margin of the amended page ...

Страница 4: ...PN B05529AE iv Revision History ...

Страница 5: ...MPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety During Installation and or Maintenance These cent...

Страница 6: ...hyl alcohol in this centrifuge nor handle or store them within the 30 cm 1 ft clearance envelope surrounding the centrifuge Mechanical Safety For safe operation of the equipment observe the following Use only the rotors and accessories designed for use in this centrifuge Before starting the centrifuge make sure that the rotor tie down screw is securely fastened Do not exceed the maximum rated spee...

Страница 7: ...andcertainsystemicfungi furtheremphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions B...

Страница 8: ...PN B05529AE viii Safety Notice Chemical and Biological Safety ...

Страница 9: ...duction xv Intended Use xv Certification xv Scope of Manual xv Conventions xvi CFC Free Centrifugation xvi Recycling Label xvi CE Mark xvii CHAPTER 1 Description 1 1 Introduction 1 1 Centrifuge Function and Safety Features 1 1 Centrifuge Function 1 1 Models 1 1 Safety Features 1 2 Chassis 1 2 Housing 1 2 Door 1 2 Rotor Chamber 1 2 Drive 1 3 Temperature Sensing and Control refrigerated models only ...

Страница 10: ...a Run 2 11 Stopping a Run 2 12 Unloading 2 12 Disabling the Audible Tones 2 13 Summary of Run Procedures 2 13 CHAPTER 3 Troubleshooting 3 1 Introduction 3 1 User Messages 3 1 Other Possible Problems 3 3 Emergency Access 3 4 CHAPTER 4 Care and Maintenance 4 1 Introduction 4 1 Maintenance 4 1 Preventive Maintenance 4 1 Cleaning 4 2 Tube Breakage 4 3 Decontamination 4 3 Sterilization and Disinfection...

Страница 11: ...s APPENDIX A Installation A 1 Introduction A 1 Installing the Instrument A 1 Transportation Safety Devices A 2 Electrical Requirements A 2 Test Run A 4 Beckman Coulter Inc Allegra X 30 Series Centrifuge Warranty ...

Страница 12: ... Power Switch 1 4 1 3 System Panels 1 4 1 4 System Keys 1 5 1 5 Program Keys 1 6 1 6 Digital Displays 1 7 2 1 Tapered Sleeve Position 2 3 2 2 Default Parameters 2 6 3 1 Screw Locations 3 4 3 2 Internal Access 3 5 A 1 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges A 3 ...

Страница 13: ...xiii Tables Tables 2 1 Acceleration Deceleration Times in minutes seconds 2 9 3 1 Error Message Chart 3 2 3 2 Troubleshooting Chart 3 3 ...

Страница 14: ...xiv Tables ...

Страница 15: ...cope of Manual This manual is designed to familiarize you with the Beckman Coulter Allegra X 30 Series centrifuges itsfunctions specifications operation androutineoperatorcareandmaintenance We recommend that you read this entire manual especially the Safety Notice section and all safety related information before operating the centrifuge or performing instrument maintenance The following introduct...

Страница 16: ...e of the back cover of this manual Typographic Conventions Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys and displays Key names for example START or ENTER and display names for example TEMP C or SPEED appear in bold type Cursorkeys used to increment values up or down when setting parameters are shown as up and down ar...

Страница 17: ...bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device CE Mark A CE mark indicates that a product has been assessed before being placed on the market and has been found to meet European Union safety health and or environmental protectio...

Страница 18: ...PN B05529AE xviii Introduction CE Mark ...

Страница 19: ...ugations Processing large numbers of small volume samples in multi well plates for concentrating tissue culture cells cloning and replicate studies in vitro cytoxicity studies receptor binding and genetic engineering experimentation Rapid sedimentation of protein precipitates large particles and cell debris Binding studies and separation of whole blood Cell isolation The centrifuges are microproce...

Страница 20: ...the centrifuge is moved while running or if it is not resting on a level surface The centrifuge feet made of rubber have been designed to minimize possible rotation in the event of a rotor mishap Chassis Housing The centrifuge housing is made of sheet steel finished with urethane paint The control panel is covered by a protective overlay made of coated polycarbonate Door The door is made of a soli...

Страница 21: ...odels only With the power on the temperature control system is activated when the door is closed The run temperature can be set between 20 and 40 C in refrigerated models If no set temperature is entered the centrifuge automatically selects the last entered temperature For the first run of a new centrifuge the instrument selects 20 C as its operating temperature A thermistor in the rotor chamber c...

Страница 22: ...l power to the centrifuge Figure 1 2 The Power Switch NOTE The power must be turned on before the chamber door can be opened Control Panel The control panel is mounted at an angle on the front of the centrifuge and includes system keys programming keys and digital displays see Figure 1 3 The panel also contains an IMBALANCE light that flashes if rotor loads are severely out of balance Figure 1 3 S...

Страница 23: ...essed to end a run It operates in two modes depending on how you press it Normal stop press and release The centrifuge decelerates to a complete stop according to the preselected deceleration curve Deceleration can be terminated and the centrifuge restarted by pressing START again The centrifuge emits a series of audible tones when the rotor reaches 0 rpm Instructions for disabling the tones are i...

Страница 24: ...es indicating that the time can be entered with the cursor keys Timed run Run time up to 9 hours and 59 minutes can be set If the minutes parameter exceeds 59 it is automatically converted into hours Continuous run If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected continuous operation is activated Time is not counted down and the run continues until the STOP key i...

Страница 25: ...ng key pressed RPM RCF or ROTOR For example if the ROTOR programming key is pressed a rotor number appears on the SPEED display During centrifugation the SPEED display shows the speed of the rotor in rpm If the RCF key is pressed while the centrifuge is running the RCF value is displayed TIME During a timed run between 1 minute and 9 hours 59 minutes the TIME display begins counting down when the ...

Страница 26: ...tual rotor speed 50 rpm of set speed actual rotor speed in 100 rpm increments or in RCF when selected Time Set time Time display to 9 hr 59 min or continuous time remaining in run timed run 1 minute or and elapsed time continuous run Acceleration 10 acceleration profiles Deceleration 10 deceleration profiles Ambient temperature range 4 to 35 C Humidity restrictions 80 noncondensing Dimensions Widt...

Страница 27: ...me Time display to 9 hr 59 min or continuous time remaining in run timed run 1 min accuracy or and elapsed time continuous run Temperature Set temperature Temperature control after equilibration Temperature display after equilibration Operating range Ambient temperature range 20 to 40 C in 1 C increments 2 5 C of set temperature chamber temperature in 1 C increments 2 to 40 Ca 10 to 35 C Accelerat...

Страница 28: ...tive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected Rotor Profile Description Refrigerated Nonrefrigerated Max Capacity mL Rotor Part Number Rotor Manual Number Max RPM Max RCF g Max RPM Max RCF g F2402Ha Fixed Angle 45 Angle rmax 82 mm 18 000 29 756 16 000 23 511 24 1 5 2 0 361171 GS TB 021 FX301 5 Fixed Angle 45 Angle rmax 100 mm 16 000b 28...

Страница 29: ...rotiter rmax 110 mm 4700 2721 4700 2721 6 ea 96 0 3 mL 2 ea 96 2 mL B01430 SX4250 Swinging Bucket rmax 172 mm 4500 3901 4200 3398 4 250 392243 GS22 TB 002 SX4400a Swinging Bucket rmax 172 mm 4700 4255 4200 3398 4 400 B01425 SX241 5 Swinging Bucket rmax 74 mm 14 000 16 244 12 500 12 950 24 1 5 2 0 392271 MMR TB 003 a Certified for biocontainment by Porton Down U K b Minimum achievable temperature a...

Страница 30: ...PN B05529AE 1 12 Description Available Rotors ...

Страница 31: ...ese centrifuges Refer to the applicable rotor manual for instructions on preparing the rotor for centrifugation To prevent condensation keep the centrifuge door closed and the power turned off O when the centrifuge is not in use NOTE If the centrifuge is used in a manner other than that specified in this manual the safety and performance of this equipment could be impaired ...

Страница 32: ...r aerosol containment WARNING The centrifuge must not be used in the vicinity of flammable liquids or vapors and such materials should not be run in the centrifuge During operation you should come within the 7 6 cm 3 in clearance envelope only to adjust instrument controls if necessary Never bring any flammable substances within the 30 cm 1 ft area surrounding the centrifuge Do not lean on the cen...

Страница 33: ...ate properly if the sleeve is missing Figure 2 1 Tapered Sleeve Position NOTE If the tapered sleeve comes off it must be replaced by a Beckman Coulter Field Service representative Call Beckman Coulter Field Service 1 800 742 2345 in the United States outside the U S contact your local Beckman Coulter office or visit us at www beckmancoulter com CAUTION Do not drop the rotor onto the drive shaft Th...

Страница 34: ...oke straight down onto the drive shaft b Tighten the tie down screw clockwise onto the drive shaft c Seat the filled buckets onto the yoke pins Be sure to fill all positions on the yoke with buckets For a fixed angle rotor the steps include the following ...

Страница 35: ...onto the drive shaft b Tighten the tie down screw clockwise onto the drive shaft c Attach the lid and tighten it Some rotors have a knob and others have a bolt for the T handle wrench NOTE Fixed angle rotors can be centrifuged without lids attached when longer tubes are used ...

Страница 36: ...ach run Entering Run Parameters When the power is applied for the initial use no previous runs default values are displayed see Figure 2 2 After the initial use the parameters of the latest previous run are displayed when power is applied When run parameters for a rotor have been entered as described below they are retained in the centrifuge memory and can be recalled by simply entering the rotor ...

Страница 37: ... ENTER Nominal parameter values time temperature speed acceleration and deceleration curves for the selected rotor are shown You can use these parameters or set new values for the run Setting Run Speed Centrifuge speed can be set for up to the maximum rated speed of the selected rotor Either revolutions per minute rpm or relative centrifugal field RCF can be used to select speed During centrifugat...

Страница 38: ...es When the time display reaches zero the run ends Continuous Run If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected continuous operation is activated Time is not counted down during continuous operation instead the infinity symbol indicating continuous operation lights up and time elapsed since the run start is displayed The run continues until the STOP key is pre...

Страница 39: ...acceleration setting 9 can be used However if delicate gradients are being run a lower setting may be needed Acceleration rates are listed in Table 2 1 1 Press the ACCEL key The ACC DEC display flashes indicating that the selected rate number can be entered with the cursor keys 2 Press the or cursor key until the required number is displayed Table 2 1 Acceleration Deceleration Times in minutes sec...

Страница 40: ...re described above 1 Check that all parameters are correct and the door is shut and latched 2 Press the ENTER key then the START key As the run begins the instrument rotor identification system compares the rotor in the chamber to the entered rotor number and the speed entered An incorrect rotor identification or set speed greater than the rotor s maximum permitted speed results in an error code a...

Страница 41: ...sed seconds When the PULSE key is released the seconds stop accumulating The ACCEL DECEL display continues to show the user entered settings during a pulse run even though the maximum rates are used If the PULSE key is pressed while the rotor is running at set speed the rotor continues running at speed until the PULSE key is released When the PULSE key is released the rotor begins decelerating to ...

Страница 42: ... key for normal deceleration as selected by the deceleration curve or Press and hold the STOP key for deceleration at the maximum rate see Table 2 1 NOTE If you hold the STOP key for more than a full second the deceleration process cannot be interrupted the centrifuge cannot be restarted until the rotor comes to a complete stop and the door is opened and closed 2 After the rotor stops spinning and...

Страница 43: ...n or off The word on or off is displayed to indicate the current setting The arrow keys toggle between on and off settings 3 Press ENTER to save the selection Summary of Run Procedures For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration For low temperature runs precool the centrifuge by running a 30 minute cycle with a precooled rotor installed a...

Страница 44: ...ss ENTER then START WARNING Never attempt to override the door interlock system while the rotor is spinning CAUTION Do not lift or move the centrifuge while the rotor is spinning 7 Wait for the set time to count down to zero or end the run by pressing or holding the STOP key 8 After the rotor stops spinning and the OPEN DOOR light comes on press the OPEN DOOR key to release the door latch open the...

Страница 45: ...fuge memory and critically interfere with future operation If a problem occurs during operation the rotor decelerates to a stop an error code appears on the SPEED display and the centrifuge emits a series of audible tones to alert the operator to the error condition Such problemsmay result from incorrect input or from anequipment malfunction Refer to Table 3 1 to determine the nature of the proble...

Страница 46: ...r turn the power off O then back on I to reset 82 83 Door does not open See Emergency Access below 84 Motor over temperature Deceleration to full stop 1 Check that ambient temperature is within a the limits shown in Specifications in CHAPTER 1 2 Check air inlets and exhausts for obstructions After the motor has cooled restart If the problem persists call Beckman Coulter Field Service b 85 through ...

Страница 47: ...while the instrument is operating 2 Make sure both ends of the power cord are securely connected call Beckman Coulter Field Service 3 Call Beckman Coulter Field Service Door does not open 1 Rotor spinning 2 Power not on 3 Source power failure 4 Latch stuck 1 Wait until the rotor stops 2 Plug in the power cord turn power on I 3 See Emergency Access below 4 See Emergency Access below Displays are bl...

Страница 48: ... mechanical injury Therefore turn the power off and disconnect the instrument from the main power source by removing its power plug from the receptacle and refer such maintenance to qualified service personnel 1 Turn the power switch to off O and disconnect the power cord from the main power source by removing its power plug from the receptacle WARNING Never attempt to override the door interlock ...

Страница 49: ...e 8 Carefully lift the centrifuge front cover until the bottom releases then pull the top towards you slightly To support the cover insert the cover bottom lip under the standoff plate inside the centrifuge CAUTION Do not allow the cover to hang down supported only by the exposed power and grounding wires or the wires will be damaged 9 Pull the latch release cable firmly to the right until the lat...

Страница 50: ...PN B05529AE 3 6 Troubleshooting Emergency Access DANGER Never try to slow or stop the rotor by hand 11 After removing the rotor replace the front panel screws and covers ...

Страница 51: ...h involve the risk of electric shock or personal injury Make sure that the power switch is off O and the centrifuge is disconnected from the main power source by removing its power plug from the receptacle and refer such servicing to qualified service personnel Do not use alcohol or other flammable substances in or near operating centrifuges Maintenance Preventive Maintenance The following procedu...

Страница 52: ...To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or paper towel 2 Clean the drive shaft shaft cavity threads and the tie down screw at least once a week using a mild detergent such as Beckman Solution 555 and a soft brush a Dilute the detergent 10 to 1 with water b Rinse thor...

Страница 53: ...e contaminated with radioactive or pathogenic solutions perform appropriate decontamination procedures Refer to Chemical Resistances IN 175 to be sure the decontamination method will not damage any part of the centrifuge Sterilization and Disinfection The centrifuge is finished with urethane paint Ethanol 70 may be used on this surface See Chemical Resistances for more information regarding chemic...

Страница 54: ...s To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts All parts must be accompanied by a signed note plainly visible on the outside of the box stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity ...

Страница 55: ...PN B05529AE 4 5 Care and Maintenance Supply List 4 Description Part Number Silicone vacuum grease 1 oz 335148 Beckman Solution 555 1 qt 339555 ...

Страница 56: ...PN B05529AE 4 6 Care and Maintenance Supply List ...

Страница 57: ...earance envelope around the centrifuge while it is running No persons should be within this clearance boundary while the centrifuge is operating Do not handle or store hazardous materials within the 30 cm 1 ft area surrounding the centrifuge 1 The centrifuge ships in a cardboard box on a wooden pallet For easy access remove the top of the box the foam insert on top of the centrifuge and then the u...

Страница 58: ...me before running the centrifuge Transportation Safety Devices A sheet of foam rubber is installed in the rotor chamber at the factory A hole in the center of the foam stabilizes the drive shaft during transport On receipt of the centrifuge remove the foam and store it in case future relocation of the centrifuge is necessary Electrical Requirements See the Nonrefrigerated Model Specifications or R...

Страница 59: ...3 Installation Electrical Requirements A Figure A 1 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges NOTE Additional clearance is required on the right side to allow to allow access to the power switch ...

Страница 60: ...er use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip Test Run NOTE The centrifuge must be plugged in and the power switch turned to on position I before the door can be opened We recommend that you make a test run to ensure that the centrifuge is in proper operating condition following shipment See CHAPTER 2 Operation for instructions on operating t...

Страница 61: ...place such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter Replacement Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to b...

Страница 62: ...PN B05529AE Warranty 2 Beckman Coulter Inc Allegra X 30 Series Centrifuge Warranty ...

Страница 63: ......

Страница 64: ...lter Inc All Rights Reserved www beckman com Related Documents Chemical Resistances for Beckman Coulter Centrifugation Products PN IN 175 Available in hard copy or electronic pdf by request Available at www beckman com ...

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