Beckman Coulter Allegra X-30 Series, Инструкция по эксплуатации

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PN B05529AE
2-9
Operation
Run Procedure
2
The
TEMP°C
display flashes, indicating that the temperature can be entered with the
cursor keys.
2
Press the ▲ or ▼ cursor key until the required run temperature is displayed.
NOTE
For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast
equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle at the
required temperature (with a precooled rotor installed) with the speed set at about 2000 rpm.
Setting Acceleration Rate
The
ACCEL
key is used to select acceleration rates that protect delicate gradients. When the
ACCEL
key is pressed, the
ACC/DEC
display flashes, indicating that one of the 10 preset acceleration rates
can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration
(setting 9) can be used. However, if delicate gradients are being run, a lower setting may be needed.
Acceleration rates are listed in Table 2.1 .
1
Press the
ACCEL
key.
• The
ACC/DEC
display flashes, indicating that the selected rate number can be entered with
the cursor keys.
2
Press the ▲ or ▼ cursor key until the required number is displayed.
Table 2.1 Acceleration/Deceleration Times (in minutes:seconds
a
)
Curve Refrigerated Models Nonrefrigerated Models
Swinging Bucket
Rotors
(4 700 rpm)
b
Fixed Angle Rotors
(18,000 rpm)
Swinging Bucket
Rotors
(4 200 rpm)
b
Fixed Angle Rotors
(16,000 rpm)
Accel Decel Accel Decel Accel Decel Accel Decel
9
8
7
6
0:30
0:36
0:53
1:38
0:24
0:35
0:55
1:38
0:53
1:36
3:03
6:04
0:46
1:33
3:03
6:02
0:38
0:37
0:45
1:23
0:24
0:32
0:52
1:28
0:44
1:24
2:45
5:24
0:43
1:24
2:43
5:21
5
4
3
2:22
3:06
4:52
2:12
3:10
4:55
9:07
12:00
18:44
9:07
12:01
18:45
2:08
2:47
4:22
2:11
2:50
4:24
8:05
10:39
16:38
8:07
10:40
16:40
2
1
0
9:51
13:10
19:37
9:46
13:02
coast
c
37:29
50:03
74:56
37:29
49:58
coast
c
8:43
11:37
17:27
8:44
11:38
coast
c
33:17
44:24
66:37
33:19
44:25
coast
c