Beckman Coulter Allegra X-30 Series, Инструкция по эксплуатации

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PN B05529AE
2-10
Operation
Run Procedure
Setting Deceleration Rate
The
DECEL
key is used to select deceleration rates that maintain optimum separation. When the
DECEL
key is pressed, the
ACC/DEC
display flashes, indicating that one of the ten preset deceleration
rates can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum brake (setting 9)
can be used. However, if delicate gradients are being run, a lower brake setting may be needed.
Deceleration rates are listed in Table 2.1 .
1
Press the
DECEL
key.
The
ACC/DEC
display flashes, indicating that the selected number can be entered with the
cursor keys.
2
Press the ▲ or ▼ cursor key until the required number is displayed.
Starting a Run
The run can be started using the parameters in memory from a previous run, or using new or
changed parameters that you enter using the procedure described above.
1
Check that all parameters are correct and the door is shut and latched.
2
Press the
ENTER
key, then the
START
key.
•
As the run begins, the instrument rotor identification system compares the rotor in the
chamber to the entered rotor number and the speed entered.
•
An incorrect rotor identification, or set speed greater than the rotor’s maximum
permitted speed, results in an error code and the centrifuge shuts down.
—
See CHAPTER 3,
Troubleshooting
for information on error codes.
— The error must be cleared and an appropriate speed entered before the centrifuge
can be started.
— Throughout the run, checks are made to ensure that the rotor does not exceed
set speed.
•
The
SPEED
display indicates the rotor speed in rpm.
— (The RCF can be checked by pressing the
RCF
key.)
a. Times are approximate; actual times vary depending on the rotor in use, the rotor load, run speed, and voltage fluctuations.
b. Maximum speed for the S2096 microtiter rotor is 3000 rpm; acceleration and deceleration times decrease accordingly.
c. Deceleration setting 0 is a no-brake coast to stop and, in some cases, may take less time than deceleration setting 1.