Bayer HealthCare ADVIA 60, Руководство оператора

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4. TECHNOLOGY
Page 4/4 075D0002-01
Diag. 4.3
4.2.1.4. Results
The lymphocytes, monocytes, and granulocytes are expressed in percents (%) and absolute
numbers (#) : LYM%, LYM#, MON%, MON#, GRA%, GRA#.
1 Leukocyte Flags
2 LYM
3 MON
4 GRA
5 Number of Cells
4.2.1.2. Diluent and lysing action
The diluent preserves and prepares the cell membrane for the differential reaction. The lyse has a
differential mode of action on cytoplasmic membranes. The lymphocyte cytoplasmic membranes
allow the release of water soluble cytoplasm and shrink the membrane around the nucleous, the
monocytes undergo an intermediate reaction, while the granulocytes have a limited reaction due
to a molecule and their cytoplasmic structure which protects them from the shrinking action of
the lyse.
4.2.1.3. Volumetric study
After the differential lysing action, ADVIA 60-
CT
analyzes the heights of each impulse, coming
from the aperture and ranked in the 256 counting channels. A curve is obtained with number of
cells on the y-axis and cell volume on the x-axis. The cells will be broken down accordingly:
* the lymphocytes between 30 - 100 fL .
* the monocytes between 100 - 150 fL .
* the granulocytes between 150 fL and a maximum (unlimited volumetrically).
Pathologic cells will, of course, place themselves in different zones in the distribution curve.
Mobile and fixed flags will alert the lab operator of the presence of such pathologic elements.
NOTE: The WBC distribution curve disappears when the WBC result is rejected.