Bayer HealthCare ADVIA 60, Руководство оператора

Страница: 33 / 128

4. TECHNOLOGY
Page 4/3 075D0002-01
4.1.2. Hemoglobin measurement principle
1 - During the STARTUP cycle an HGB blank test sequence including two blank measures is run.
If the difference between these two measures is too large a third measurement is performed.
2 - On every cycle an Hgb blank is carried out on diluent and compared to the previous HGB blank
analysis.
3 - 0.60 mL of lyse agent is added to the 2.5 mL of 1/250 dilution.
4 - The hemoglobin freed by the lyse of the red blood cells combines with potassium cyanide to
form chromogenous cyanmethemoglobin compound.
5 - The compound is then measured by spectrophotometry, through the WBC cuvette at 550 nm.
6 - The result is given with the units set up in Section 8 "Instrument Configuration".
4.1.3. Hematocrit measurement principle
1 - The height of the impulse generated by the passage of a cell through the microaperture is
directly proportional to the volume of the analyzed cell.
2 - The hematocrit is measured as a function of the numeric integration of the MCV.
3 - The result is given with the units set up in Section 8 "Instrument Configuration".
4.2. CELL DISTRIBUTION STUDY
ADVIA 60-
CT
carries out volumetric distributions (histograms) for WBC and RBC on 256 analysis
channels, and 128 channels for PLT with the following measuring range:
* WBC = approximately 30 to 460 fL.
* RBC = approximately 25 to 300 fL.
* Plt = approximately 2 to 33 fL.
4.2.1. White blood cell distribution
The WBC volumetric distribution study reveals the following three leukocyte subpopulations :
Lymphocytes, Monocytes, Granulocytes.
4.2.1.1. Analysis principle
The principle is based on the volumetric study of leukocytes after use of a (patented) diluent and
a lysing reagent.
NOTE:
The lysing action varies according to the temperature of the dilution (ambient
temperature). In order to correct these fluctuations within the operating temperature
range (18°C to 32°C, 65°F to 90°F), a temperature sensor is placed on the diluent circuit
and the position of the separation thresholds varies according to the temperature.
NOTE:
HGB reference blank measurement will occur if the operator:
- has required a CALIBRATION operation.
- has required a QC operation.
- has left the instrument more than 10 minutes after the STARTUP.
- has left the instrument more than 60 minutes after ANALYSIS.
- has not carried out the STARTUP cycle after switching on the instrument.